In vitro method for detection of immortalized cells in human retinal pigment epithelial cells.
| Project/Area Number |
25871244
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Drug development chemistry
Ophthalmology
|
|---|
| Research Institution | National Institute of Health Sciences (2014-2015)
Foundation for Biomedical Research and Innovation (2013) |
|---|
Principal Investigator |
Kuroda Takuya 国立医薬品食品衛生研究所, 再生・細胞医療製品部, 研究員 (70648857)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 不死化細胞 / 網膜色素上皮細胞 / 造腫瘍性 / iPS細胞 / 造腫瘍性細胞 |
|---|
| Outline of Final Research Achievements |
Human pluripotent stem cells (hPSCs), i.e. human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), are able to self-renew and differentiate into multiple cell types. Thus, numerous attempts have been made to utilize hPSCs in regenerative medicine/cell therapy. However hPSCs have tumorigenic potential. Therefore, residual undifferentiated hPSCs or contamination of the transformed cells in products that would eventually proliferate and form a teratoma is one of the most obvious safety issues to develop cell therapy hPSC-derived products. In the present study, we identified a new immortalized RPE cell marker and developed new qRT-PCR method to detect contamination of human RPE cells with immortalized RPE cells. This qRT-PCR assay can detect as few as 3% of immortalized RPE cells in normal RPE cells. This in vitro qRT-PCR method is expected to contribute to process validation and quality control of cell therapy products derived from hPSCs.
|
Report
(4 results)
Research Products
(14 results)
