Improvement of in situ surface modification techniques for manipulating neuronal networks
| Project/Area Number |
25880021
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Life / Health / Medical informatics
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| Research Institution | Tohoku University (2014)
Waseda University (2013) |
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Principal Investigator |
YAMAMOTO Hideaki 東北大学, 学際科学フロンティア研究所, 助教 (10552036)
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| Project Period (FY) |
2013-08-30 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 神経細胞 / 自己組織化単分子膜 / 酸化チタン / TiO2 / 表面改質 / バイオインターフェース / Si-tag / RPL2 |
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| Outline of Final Research Achievements |
Titanium dioxide (TiO2) photocatalysis can be applied to pattern proteins and cells under aqueous solution. Here, we extended the application of this technique to pattern primary neurons. We employed ribosomal protein L2 (RPL2) to stably bind laminin to TiO2. We found that a protein complex consisting of laminin/anti-laminin antibody/protein A-RPL2 is spontaneously formed by simply mixing the precursor proteins in solution phase, and that the surface coated with the protein complex supports stable growth of rat hippocampal neurons. Finally, we showed that the cells can be selectively grown on the protein complex patterned with the TiO2-assisted method. The protocol established here is a unique combination of a top-down micropatterning of the surface using TiO2 photocatalysis and a bottom-up self-assembly of biomolecules, which can be further applied to pattern a wide range of proteins and cells. (Sekine, Yamamoto et al., e-J Surf Sci Nanotechnol 2015)
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Report
(3 results)
Research Products
(52 results)
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[Journal Article] In situ laser micropatterning of proteins for dynamically arranging living cells2013
Author(s)
*Kazunori Okano, Ai Matsui, Yasuyo Maezawa, Ping-Yu Hee, Mie Matsubara, Hideaki Yamamoto, Yoichiroh Hosokawa, Hiroshi Tsubokawa, Yaw-Kuen Li, Fu-Jen Kao, Hiroshi Masuhara
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Journal Title
Lab Chip.
Volume: Vol. 13, No. 20
Issue: 20
Pages: 4078-4086
DOI
Related Report
Peer Reviewed
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[Presentation] マイクロパターン上への細胞接着過程のタイムラプス解析 －緑茶カテキンを含む培養液中でのがん細胞と正常細胞の比較－
Author(s)
坂本留実, 益田顕太朗, 柿沼瑛介, 伊藤耕作, 池滝健太郎, 松崎賢寿, 吉川洋史, 中林誠一郎, 山本英明, 佐藤裕子, 谷井孝至
Organizer
第61回応用物理学会春期学術講演会
Place of Presentation
青山大学, 神奈川
Related Report
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[Presentation] Green-tea catechin alters the adhesion state in cancer cells, but not in normal cells
Author(s)
K. Masuda, E. Kakinuma, R. Sakamoto, K. Ito, K. Iketaki, T. Matsuzaki, H. Yoshikawa, S. Nakabayashi, H. Yamamoto, H. T. Chi, Y. Sato, T. Tanii
Organizer
5th Int. Conf. on O-CHA (Tea) Culture and Science (ICOS 2013)
Place of Presentation
Granship, Shizuoka
Related Report
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[Presentation] Suppressive effect of epigallocatechin-3-gallate on adhesion of metastatic cancer cells studied by reflection interference contrast microscopy measurement of micropatterned cells
Author(s)
R. Sakamoto, K. Masuda, E. Kakinuma, K. Ito, K. Iketaki, T. Matsuzaki, H. Yoshikawa, S. Nakabayashi, H. Yamamoto, Y. Sato, T. Tanii
Organizer
5th Int. Conf. on O-CHA (Tea) Culture and Science (ICOS 2013)
Place of Presentation
Granship, Shizuoka
Related Report
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