| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
I fabricated microfiber, which served as a scaffold for mammalian cells. The microfibers were consists of nanofibrous cellulose secreted by bacteria (bacterial cellulose). The aim of this study is to fabricate vessel-like structure by culturing cells on the microfibers and three-dimensional cellular constructs. I achieved fabrication of three-dimensional cellular constructs which had heterocellular hierarchic structure. I also found an enzyme suitable for degrading the bacterial cellulose fibers after the fabrication of cellular construct. Furthermore, the nutrition diffusion model was fabricated to calculate the maximum culturable time of the cellular constructs without necrosis.
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