Development of early diagnosis method for colorectal cancer using supercritical fluid chromatography/ mass spectrometry

Research Project

Project/Area Number	25882023
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Biomolecular chemistry
Research Institution	Kyoto University (2014) Kobe University (2013)
Principal Investigator	MATSUBARA Atsuki 京都大学, 医学(系)研究科(研究院), 薬剤師 (90709677)
Project Period (FY)	2013-08-30 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000) Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	超臨界流体クロマトグラフィ / メタボロミクス / 質量分析 / 超臨界流体抽出 / 超臨界流体クロマトグラフィー / バイオマーカー
Outline of Final Research Achievements	Metabolome analysis is one of the promising approach for biomarker discovery and pathophysiological analysis. However, due to analytical difficulty, some kind of hydrophobic metabolites which are deeply involved in various diseases have not been targeted for analysis. In this study, supercritical fluid chromatography and extraction were applied for construction of analytical system for these important metabolites to construct early diagnosis of cancers.