In vivo measurement of mitochondrial function in human skeletal muscle

• Project/Area Number: 25882041
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Sports science
• Research Institution: Ritsumeikan University
• Principal Investigator: SUGA Tadashi 立命館大学, 立命館グローバル・イノベーション研究機構, 研究員 (30708673)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 運動 / 骨格筋 / ミトコンドリア機能 / 磁気共鳴分光法 / 近赤外分光法 / 加齢 / 糖尿病 / ミトコンドリア

Outline of Final Research Achievements

The present study aimed to examine the availability of a noninvasive method for evaluating mitochondrial function in skeletal muscle by combining phosphorus magnetic resonance spectroscopy (31P-MRS) and near-infrared spectroscopy (NIRS). Ischemia of the lower limb was induced for 15 min by using a rapid inflator with a pressure cuff placed around the thigh. Under this condition, the reduced speeds of phosphocreatine (PCr) and oxy-hemoglobin/myoglobin (Hb/Mb) were measured using 31P-MRS and NIRS, respectively. The rate of these speeds was calculated as mitochondrial coupling capacity. The present study found that the reduced speeds of PCr and oxy-Hb/Mb were significantly higher in endurance-trained subjects than in untrained subjects, whereas mitochondrial coupling capacity did not differ significantly between endurance-trained subjects and untrained subjects. Thus, mitochondrial coupling capacity may not be appropriate in evaluating mitochondrial function in skeletal muscle.

Research Products

• [Journal Article] Ischemic preconditioning accelerates muscle deoxygenation dynamics and enhances exercise endurance during the work-to-work test (2015)
  • Author(s): Kido K, Suga T, Tanaka D, Honjo T, Homma T, Fujita S, Hamaoka T, Isaka T
  • Journal Title: Physiological Reports Volume: 3 Issue: 5 Pages: 1-10
  • DOI: 10.14814/phy2.12395
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Intramyocellular lipid is increased in the skeletal muscle of patients with dilated cardiomyopathy with lowered exercise capacity. (2014)
  • Author(s): Hirabayashi K, Kinugawa S, Yokota T, Takada S, Fukushima A, Suga T, Takahashi M, Ono T, Morita N, Omokawa M, Harada K, Oyama-Manabe N, Shirato H, Matsushima S, Okita K, Tsutsui H
  • Journal Title: International Journal of Cardiology Volume: 176 Issue: 3 Pages: 1110-1112
  • DOI: 10.1016/j.ijcard.2014.07.113
  • NAID: 120006809251
  • Peer Reviewed
• [Journal Article] Pioglitazone ameliorates the lowered exercise capacity and impaired mitochondrial function of the skeletal muscle in type 2 diabetic mice. (2014)
  • Author(s): Takada S, Hirabayashi K, Kinugawa S, Yokota T, Matsushima S, Suga T, Kadoguchi T, Fukushima A, Homma T, Mizushima W, Masaki Y, Furihata T, Katsuyama R, Okita K, Tsutsui H
  • Journal Title: European Journal of Pharmacology Volume: 740 Pages: 690-696
  • DOI: 10.1016/j.ejphar.2014.06.008
  • NAID: 120005476207
  • Peer Reviewed
• [Journal Article] (Pro)renin receptor in skeletal muscle is involved in the development of insulin resistance associated with postinfarct heart failure in mice (2014)
  • Author(s): Fukushima A, Kinugawa S, Takada S, Matsushima S, Sobirin MA, Ono T, Takahashi M, Suga T, Homma T, Masaki Y, Furihata T, Kadoguchi T, Yokota T, Okita K, Tsutsui H
  • Journal Title: American Journal of Physiology Endocrinology and Metabolism Volume: 307 Issue: 6 Pages: E503-E514
  • DOI: 10.1152/ajpendo.00449.2013
  • Peer Reviewed
• [Journal Article] Combination of exercise training and diet restriction normalizes limited exercise capacity and impaired skeletal muscle function in diet-induced diabetic mice. (2014)
  • Author(s): Suga T, Kinugawa S, Takada S, Kadoguchi T, Fukushima A, Homma T, Masaki Y, Furihata T, Takahashi M, Sobirin MA, Ono T, Hirabayashi K, Yokota T, Tanaka S, Okita K, Tsutsui H.
  • Journal Title: Endocrinology Volume: 155 Issue: 1 Pages: 68-80
  • DOI: 10.1210/en.2013-1382
  • Peer Reviewed
• [Journal Article] Decreased serum brain-derived neurotrophic factor levels are correlated with exercise intolerance in patients with heart failure. (2013)
  • Author(s): Fukushima A1, Kinugawa S, Homma T, Masaki Y, Furihata T, Yokota T, Matsushima S, Abe T, Suga T, Takada S, Kadoguchi T, Katsuyama R, Oba K, Okita K, Tsutsui H.
  • Journal Title: International Journal of Cardiology Volume: 168 Issue: 5 Pages: e142-e144
  • DOI: 10.1016/j.ijcard.2013.08.073
  • Peer Reviewed
• [Journal Article] Increased plasma soluble (pro) renin receptor levels are correlated with renal dysfunction in patients with heart failure. (2013)
  • Author(s): Fukushima A1, Kinugawa S, Homma T, Masaki Y, Furihata T, Abe T, Suga T, Takada S, Kadoguchi T, Okita K, Matsushima S, Tsutsui H
  • Journal Title: International Journal of Cardiology Volume: 168 Issue: 4 Pages: 4313-4314
  • DOI: 10.1016/j.ijcard.2013.04.176
  • Peer Reviewed
• [Journal Article] Activation of invariant natural killer T cells by α-galactosylceramide ameliorates myocardial ischemia/reperfusion injury in mice. (2013)
  • Author(s): Homma T, Kinugawa S, Takahashi M, Sobirin MA, Saito A, Fukushima A, Suga T, Takada S, Kadoguchi T, Masaki Y, Furihata T, Taniguchi M, Nakayama T, Ishimori N, Iwabuchi K, Tsutsui H.
  • Journal Title: Journal of Molecular and Cellular Cardiology Volume: 62 Pages: 179-188
  • DOI: 10.1016/j.yjmcc.2013.06.004
  • Peer Reviewed
• [Journal Article] Systemic Oxidative Stress Is Associated With Lower Aerobic Capacity and Impaired Skeletal Muscle Energy Metabolism in Patients With Metabolic Syndrome (2013)
  • Author(s): Yokota T, Kinugawa S, Yamato M, Hirabayashi K, Suga T, Takada S, Harada K, Morita N, Oyama-Manabe N, Kikuchi Y, Okita K, Tsutsui H.
  • Journal Title: Diabetes Care Volume: 36 Issue: 5 Pages: 1341-1346
  • DOI: 10.2337/dc12-1161
  • Peer Reviewed
• [Journal Article] Angiotensin II Receptor Blocker Improves the Lowered Exercise Capacity and Impaired Mitochondrial Function of the Skeletal Muscle in Type 2 Diabetic Mice. (2013)
  • Author(s): Takada S, Kinugawa S, Hirabayashi K, Suga T, Yokota T, Takahashi M, Fukushima A, Homma T, Ono T, Sobirin MA, Masaki Y, Mizushima W, Kadoguchi T, Okita K, Tsutsui H.
  • Journal Title: Journal of Applied Physiology Volume: 114 Issue: 7 Pages: 844-857
  • DOI: 10.1152/japplphysiol.00053.2012
  • Peer Reviewed
• [Presentation] アンジオテンシンII受容体阻害薬はストレプトゾトシン誘発性糖尿病マウスにおける運動能力低下と骨格筋ミトコンドリア機能障害を改善する (2014)
  • Author(s): 菅 唯志，絹川真太郎，高田真吾，門口智泰，筒井裕之
  • Organizer: 第69回日本体力医学会大会
  • Place of Presentation: 長崎大学文京キャンパス（長崎)
  • Year and Date: 2014-09-20
• [Presentation] 酪酸は運動能力および骨格筋ミトコンドリア機能を増加する
  • Author(s): 菅 唯志，絹川真太郎，高田真吾，門口智泰，伊坂忠夫，沖田孝一，筒井裕之
  • Organizer: 第68回日本体力医学会大会
  • Place of Presentation: 東京都（日本教育会館学術総合センター共立会館）
• [Presentation] アンジオテンシンII由来の酸化ストレスは骨格筋異常を引き起こす
  • Author(s): 門口智泰，絹川真太郎，菅 唯志，高田真吾，沖田孝一
  • Organizer: 第68回日本体力医学会大会
  • Place of Presentation: 東京都（日本教育会館学術総合センター共立会館）
• [Presentation] 慢性心不全患者における脳由来神経栄養因子の低下は運動耐容能の低下に関連する
  • Author(s): 高田真吾，絹川真太郎，菅 唯志，門口智泰，沖田孝一，筒井裕之
  • Organizer: 第68回日本体力医学会大会
  • Place of Presentation: 東京都（日本教育会館学術総合センター共立会館）