Design of Vesicle Membranes for Ensemble Effect-Like Recognition and Conversion Process
Project/Area Number |
25889039
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Biofunction/Bioprocess
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Research Institution | Osaka University |
Principal Investigator |
SUGA Keishi 大阪大学, 基礎工学研究科, 助教 (00709800)
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Project Period (FY) |
2013-08-30 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | リポソーム / ベシクル / 自己組織化 / "協奏的"分子認識 / 表面増強ラマン / RNA / グアニジニウム / 環化付加反応 / グアニジニウム基 / ”協奏的”分子認識 / 表面増強ラマン分光解析 |
Outline of Final Research Achievements |
The self-assembled membranes of amphiphilic molecules were designed with phospholipid, cholesterol, fatty acid, surfactant, etc., and were applied to the molecular recognition and conversion processes. The phospholipid-modified Au nanoparticles (Au@PL) were developed for high-sensitive analysis of the dynamic behaviors of membranes. Au@PL showed the enhanced Raman spectra with >100-fold larger intensity. With fluorescent prove analysis, various kinds of vesicle membranes could be characterized. Using such a designed vesicle, the recognition and conversion processes carried out. In the case of 1,3-dipolarcycloaddition reaction, the hydrophobic substrate (BNO) and the hydrophilic substrate (EMI) were accumulated on the membrane, where the membrane surface was dehydrated due to the distribution of BNO. The relative reaction constant increased to 2.7-fold, indicating that the membrane surface can applied to the recognition and conversion processes.
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Report
(3 results)
Research Products
(48 results)
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[Journal Article] Microfluidic platforms with monolithically integrated hierarchical apertures for the facile and rapid formation of cargo-carrying vesicles2014
Author(s)
Cho, H., Kim, J., Suga, K., Ishigami, T., Park, H., Bang, J. W., Seo, S., Choi, M., Chang, P. –S., Umakoshi, H., Jung, H. –S., Suh, K. –Y.
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Journal Title
Lab on a chip - Miniaturisation for Chemistry and Biology
Volume: 15
Issue: 2
Pages: 373-377
DOI
Related Report
Peer Reviewed / Open Access
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