| Project/Area Number |
25890007
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Kanazawa University |
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Principal Investigator |
OGAI Kazuhiro 金沢大学, 健康増進科学センター, 助教 (40706983)
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| Project Period (FY) |
2013-08-30 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 神経再生 / ゼブラフィッシュ / LIF / STAT3 / GAP-43 / 視神経再生 |
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| Outline of Final Research Achievements |
This research has been aimed at investigating the beneficial effect of leukemia inhibitory factor (LIF) on zebrafish optic nerve regeneration.
The LIF mRNA was upregulated within 3 days after optic nerve transection followed by the activation of STAT3 at 5 days. The activation of STAT3 in turn upregulated GAP-43, which is required for nerve elongation. Meanwhile, the knockdown of LIF attenuated the STAT3 activation and GAP-43 upregulation, and delayed the functional recovery during optic nerve regeneration. These result would tell that the upregulation of LIF after optic nerve transection may have a beneficial effect on optic nerve regeneration in adult zebrafish, which in turn implies the therapeutic strategies of LIF application against central nervous system injuries.
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