Molecular mechanism of temporal AMPA-type glutamate receptor internalization during hippocampal long-term potentiation

• Project/Area Number: 25890009
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Kyoto University
• Principal Investigator: TANAKA Hiromitsu 京都大学, 理学(系)研究科(研究院), 助教 (30705447)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 神経科学 / 分子生物学 / ライブイメージング / シナプス可塑性 / 長期増強 / 長期抑圧 / グルタミン酸受容体 / 全反射顕微鏡 / 細胞生物学 / 海馬長期増強現象 / AMPA型グルタミン酸受容体 / シナプス形成

Outline of Final Research Achievements

The efficacy of synaptic transmission changes depending on the pattern of neuronal activity. This synaptic plasticity has been regarded as a cellular basis of learning and memory. In this study, we have suggested that the amount of a subtype of AMPA-type glutamate receptors (AMPA receptors) on postsynaptic membrane is reduced together with dynamin during hippocampal long-term potentiation by experiments using total internal reflection fluorescence microscopy. In addition, we have developed a new method to visualize individual endocytosis of AMPA receptors.

Research Products

• [Journal Article] Live-cell imaging of receptors around postsynaptic membrane formed on adhesion-protein-coated glass (2014)
  • Author(s): Tanaka, H., Fujii, S. & Hirano, T.
  • Journal Title: Nature Protocols Volume: 9 Issue: 1 Pages: 76-89
  • DOI: 10.1038/nprot.2013.171
  • Peer Reviewed
• [Presentation] シナプス後膜周辺におけるAMPA受容体の個別のエンドサイトーシスの可視化と解析 (2014)
  • Author(s): 藤井俊平、田中洋光、平野丈夫
  • Organizer: 生理学研究所研究会
  • Place of Presentation: 生理学研究所 (岡崎)
  • Year and Date: 2014-12-03
• [Presentation] Live-cell imaging of individual endocytosis of AMPA receptor around postsynaptic membrane (2014)
  • Author(s): Shumpei FUJII, Hiromitsu TANAKA, Tomoo HIRANO
  • Organizer: Neuroscience 2014
  • Place of Presentation: Washington, D.C. (USA)
  • Year and Date: 2014-11-15
• [Presentation] シナプス後膜周辺における神経伝達物質受容体の個別のエンドサイトーシスの可視化 (2014)
  • Author(s): 藤井俊平、田中洋光、平野丈夫
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜 (横浜)
  • Year and Date: 2014-09-11
• [Presentation] New live-cell imaging system of neurotransmitter receptors (2014)
  • Author(s): Hiromitsu Tanaka, Shumpei Fujii, Tomoo Hirano
  • Organizer: New Frontier of Molecular Neuropathology 2014
  • Place of Presentation: 東京
• [Presentation] シナプス後膜内外でのグルタミン酸受容体動態の可視化 (2013)
  • Author(s): Tomoo Hirano, Hiromitsu Tanaka, Shumpei Fujii
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 神戸
• [Remarks] 所属研究室のホームページ
  • URL: http://www.neurosci.biophys.kyoto-u.ac.jp/main.html
• [Remarks] 所属研究室のホームページ
  • URL: http://www.neurosci.biophys.kyoto-u.ac.jp/main.html