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Development of CRISPR/Cas system enabling advanced genome editing on chromosome level

Research Project

Project/Area Number 25890014
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field System genome science
Research InstitutionHiroshima University

Principal Investigator

SAKUMA TETSUSHI  広島大学, 理学(系)研究科(研究院), 特任助教 (90711143)

Project Period (FY) 2013-08-30 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsゲノム編集 / CRISPR/Cas9 / ゲノム生物工学 / 遺伝子ノックイン / CRISPR/Cas
Outline of Final Research Achievements

We developed a vector system enabling advanced genome editing such as simultaneous disruption of multiple genes and large chromosomal deletion, and evaluated its applicability in cultured cells and mice. In cultured cells, multiple targeted mutagenesis for up to seven genes and chromosomal deletion were demonstrated. In addition, it was shown that simultaneous gene disruption at multiple gene loci was feasible in mouse embryos by single plasmid injection using an all-in-one vector carrying FokI-dCas9, which is known as a highly-specific variant of Cas9.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • Research Products

    (147 results)

All 2015 2014 2013 Other

All Journal Article (35 results) (of which Peer Reviewed: 35 results,  Open Access: 20 results,  Acknowledgement Compliant: 11 results) Presentation (107 results) (of which Invited: 13 results) Book (2 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Desmocollin-2 alone forms functional desmosomal plaques, with the plaque formation requiring the juxtamembrane region and plakophilins2015

    • Author(s)
      Fujiwara M, Nagatomo A, Tsuda M, Obata S, Sakuma T, Yamamoto T, Suzuki ST
    • Journal Title

      J Biochem

      Volume: -

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas92015

    • Author(s)
      Hara S, Tamano M, Yamashita S, Kato T, Saito T, Sakuma T, Yamamoto T, Inui M, Takada S
    • Journal Title

      Sci Rep

      Volume: -

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes2015

    • Author(s)
      Nakagawa Y, Sakuma T, Sakamoto T, Ohmuraya M, Nakagata N, Yamamoto T
    • Journal Title

      BMC Biotechnol

      Volume: - Issue: 1 Pages: 33-33

    • DOI

      10.1186/s12896-015-0144-x

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cloning-free CRISPR/Cas system facilitates functional cassette knockin in mice2015

    • Author(s)
      Aida T, Chiyo K, Usami T, Ishikubo H, Imahashi R, Wada Y, Tanaka K, Sakuma T, Yamamoto T, Tanaka K
    • Journal Title

      Genome Biol

      Volume: 16 Issue: 1 Pages: 87-87

    • DOI

      10.1186/s13059-015-0653-x

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Homeolog-specific targeted mutagenesis in Xenopus laevis using TALENs2015

    • Author(s)
      Nakade S, Sakuma T, Sakane Y, Hara Y, Kurabayashi A, Kashiwagi K, Kashiwagi A, Yamamoto T, Obara M
    • Journal Title

      In Vitro Cell Dev Biol Anim

      Volume: - Issue: 9 Pages: 879-884

    • DOI

      10.1007/s11626-015-9912-0

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine2015

    • Author(s)
      Kawai N, Ogura Y, Ikuta T, Saiga H, Hamada M, Sakuma T, Yamamoto T, Satoh N, Sasakura Y
    • Journal Title

      Developmental Biology

      Volume: 印刷中 Issue: 1 Pages: 43-56

    • DOI

      10.1016/j.ydbio.2015.03.018

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A High Excision Potential of TALENs for Integrated DNA of HIV-Based Lentiviral Vector2015

    • Author(s)
      Ebina H, Kanemura Y, Misawa N, Sakuma T, Kobayashi T, Yamamoto T, Koyanagi Y
    • Journal Title

      PLoS One

      Volume: 10 Issue: 3 Pages: e0120047-e0120047

    • DOI

      10.1371/journal.pone.0120047

    • NAID

      120005619759

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish.2015

    • Author(s)
      Hisano Y, Sakuma T, Nakade S, Ohga R, Ota S, Okamoto H, Yamamoto T, Kawahara A.
    • Journal Title

      Sci Rep.

      Volume: 5 Issue: 1 Pages: 8841-8841

    • DOI

      10.1038/srep08841

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tailor-made TALEN system for highly efficient targeted gene replacement in the rice blast fungus2015

    • Author(s)
      Arazoe T, Ogawa T, Miyoshi K, Yamato T, Ohsato S, Sakuma T, Yamamoto T, Arie T, Kuwata S
    • Journal Title

      Biotechnology and Bioengineering

      Volume: - Issue: 7 Pages: 1335-1342

    • DOI

      10.1002/bit.25559

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The microtubule depolymerizing activity of a mitotic kinesin protein KIF2A drives primary cilia disassembly coupled with cell proliferation2015

    • Author(s)
      Miyamoto T, Hosoba K, Ochiai H, Royba E, Izumi H, Sakuma T, Yamamoto T, Matsuura S
    • Journal Title

      Cell Reports

      Volume: 10 Issue: 5 Pages: 664-673

    • DOI

      10.1016/j.celrep.2015.01.003

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Unliganded thyroid hormone receptor alpha regulates developmental timing via gene repression in Xenopus tropicalis2015

    • Author(s)
      J. Choi, K. T. Suzuki, T. Sakuma, L. Shewade, T. Yamamoto and D. R. Buchholz
    • Journal Title

      Endocrinology

      Volume: 156 Issue: 2 Pages: 735-44

    • DOI

      10.1210/en.2014-1554

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient iPS Cells by TALEN and CRISPR-Cas92015

    • Author(s)
      Hongmei Lisa Li
    • Journal Title

      Stem Cell Reports

      Volume: 4 Issue: 1 Pages: 143-154

    • DOI

      10.1016/j.stemcr.2014.10.013

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas92014

    • Author(s)
      S. Nakade, T. Tsubota, Y. Sakane, S. Kume, N. Sakamoto, M. Obara, T. Daimon, H. Sezutsu, T. Yamamoto, T. Sakuma and K. T. Suzuki
    • Journal Title

      Nat Commun

      Volume: 5 Issue: 1 Pages: 5560-5560

    • DOI

      10.1038/ncomms6560

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Stochastic promoter activation affects Nanog expression variability in mouse embryonic stem cells2014

    • Author(s)
      Ochiai, H., Sugawara, T., Sakuma, T., Yamamoto, T.
    • Journal Title

      Scientific Reports

      Volume: 4 Issue: 1 Pages: 7125-7125

    • DOI

      10.1038/srep07125

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Targeted gene disruption by use of transcription activator-like effector nuclease (TALEN) in the water flea Daphnia pulex2014

    • Author(s)
      Chizue Hiruta, Yukiko Ogino, Tetsushi Sakuma, Kenji Toyota, Shinichi Miyagawa, Takashi Yamamoto, Taisen Iguchi
    • Journal Title

      BMC Biotechnology

      Volume: 14 Issue: 1 Pages: 95-95

    • DOI

      10.1186/s12896-014-0095-7

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Simple knockout by electroporation of engineered endonucleases into intact rat embryos2014

    • Author(s)
      Kaneko T, Sakuma T, Yamamoto T, Mashimo T
    • Journal Title

      Sci Rep

      Volume: 4 Issue: 1 Pages: 6382-6382

    • DOI

      10.1038/srep06382

    • NAID

      120005477670

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sterol side chain reductase 2 is a key enzyme in the biosynthesis of cholesterol, the common precursor of toxic steroidal glycoalkaloids in potato2014

    • Author(s)
      Sawai, S., Ohyama, K., Yasumoto, S., Seki, H., Sakuma, T., Yamamoto, T., Takebayashi, Y., Kojima, M., Sakakibara, H., Aoki, T., Muranaka, T., Saito, K., Umemoto, N.
    • Journal Title

      The Plant Cell

      Volume: 26 Issue: 9 Pages: 3763-3774

    • DOI

      10.1105/tpc.114.130096

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies2014

    • Author(s)
      Kazuki Y, Yakura Y, Abe S, Osaki M, Kajitani N, Kazuki K, Takehara S, Honma K, Suemori H, Yamazaki S, Sakuma T, Toki T, Shimizu R, Nakauchi H, Yamamoto T, Oshimura M
    • Journal Title

      Scientific reports

      Volume: 4 Issue: 1 Pages: 6136-6136

    • DOI

      10.1038/srep06136

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step.2014

    • Author(s)
      Ninagawa S, Okada T, Sumitomo Y, Kamiya Y, Kato K, Horimoto S, Ishikawa T, Takeda S, Sakuma T, Yamamoto T, Mori K
    • Journal Title

      J Cell Biol.

      Volume: 206 Issue: 3 Pages: 347-356

    • DOI

      10.1083/jcb.201404075

    • NAID

      120005468381

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems2014

    • Author(s)
      A. Yasue, S. N. Mitsui, T. Watanabe, T. Sakuma, S. Oyadomari, T. Yamamoto, S. Noji, T. Mito and E. Tanaka
    • Journal Title

      Sci Rep

      Volume: 4 Issue: 1 Pages: 5705-5705

    • DOI

      10.1038/srep05705

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system2014

    • Author(s)
      Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T
    • Journal Title

      Sci Rep

      Volume: 4 Issue: 1 Pages: 5400-5400

    • DOI

      10.1038/srep05400

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Application of oocyte cryopreservation technology in TALEN-mediated mouse genome editing2014

    • Author(s)
      Nakagawa Y, Sakuma T, Nakagata N, Yamasaki S, Takeda N, Ohmuraya M, Yamamoto T
    • Journal Title

      Exp Anim

      Volume: -

    • NAID

      130004677827

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Germ cell mutations of the ascidian Ciona intestinalis with TALE nucleases.2014

    • Author(s)
      Yoshida K, Treen N, Hozumi A, Sakuma T, Yamamoto T, Sasakura Y.
    • Journal Title

      Genesis

      Volume: in press Issue: 5 Pages: 431-439

    • DOI

      10.1002/dvg.22770

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] FAST-id system for enrichment of cells with TALEN-induced mutations and large deletions2014

    • Author(s)
      Tokumasu D, Sakuma T, Hayashi Y, Hosoi S, Hiyama E, Yamamoto T
    • Journal Title

      Genes to Cells

      Volume: 19 Issue: 5 Pages: 419-431

    • DOI

      10.1111/gtc.12142

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] TALEN-mediated single-base-pair editing identification of an intergenic mutation upstream of BUB1B as causative of PCS (MVA) syndrome.2014

    • Author(s)
      Ochiai H, Miyamoto T, Kanai A, Hosoba K, Sakuma T, Kudo Y, Asami K, Ogawa A, Watanabe A, Kajii T, Yamamoto T, Matsuura S.
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 111(4) Issue: 4 Pages: 1461-6

    • DOI

      10.1073/pnas.1317008111

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tissue-specific and ubiquitous gene knockouts in Ciona by electroporating TALENs provide new approaches to investigate gene functions2014

    • Author(s)
      Treen, N., Yoshida, K., Sakuma, T., Sasaki, H., Kawai, N., Yamamoto, T., Sasakura, Y.
    • Journal Title

      Development

      Volume: 141 Issue: 2 Pages: 481-487

    • DOI

      10.1242/dev.099572

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nuclease-mediated genome editing: At the front-line of functional genomics technology.2014

    • Author(s)
      Sakuma, T., and Woltjen, K.
    • Journal Title

      Development, growth & differentiation

      Volume: 56 Issue: 1 Pages: 2-13

    • DOI

      10.1111/dgd.12111

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Versatile strategy for isolating TALEN-mediated knockout mutants in Caenorhabditis elegans.2014

    • Author(s)
      Sugi T, Sakuma T, Ohtani T and Yamamoto T
    • Journal Title

      Dev Growth Differ

      Volume: 56 Issue: 1 Pages: 78-85

    • DOI

      10.1111/dgd.12108

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of transcription activator-like effector nucleases2014

    • Author(s)
      Sakane, Y., Sakuma, T., Kashiwagi, K., Kashiwagi, A., Yamamoto, T. and Suzuki, K. T.
    • Journal Title

      Dev Growth Differ

      Volume: 56(1) Issue: 1 Pages: 108-114

    • DOI

      10.1111/dgd.12105

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Targeted mutagenesis in sea urchin embryos using TALENs.2014

    • Author(s)
      Hosoi S, Sakuma T, Sakamoto N and Yamamoto T
    • Journal Title

      Dev Growth Differ

      Volume: 56 Issue: 1 Pages: 92-97

    • DOI

      10.1111/dgd.12099

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcription activator-like effector nucleases efficiently disrupt the target gene in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration.2014

    • Author(s)
      Hayashi T, Sakamoto K, Sakuma T, Yokotani N, Inoue T, Kawaguchi E, Agata K, Yamamoto T, Takeuchi T
    • Journal Title

      Development, Growth and Differentiation

      Volume: 56 Issue: 1 Pages: 115-121

    • DOI

      10.1111/dgd.12103

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] TALEN-induced gene knock out in Drosophila.2014

    • Author(s)
      Kondo T
    • Journal Title

      Development Growth and Differentiation

      Volume: 56:1 Issue: 1 Pages: 86-91

    • DOI

      10.1111/dgd.12097

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Screening Methods to Identify TALEN-Mediated Knockout Mice2014

    • Author(s)
      Nakagawa Y, Yamamoto T, Suzuki KI, Araki K, Takeda N, Ohmuraya M, Sakuma T
    • Journal Title

      Experimental Animals

      Volume: 63 Issue: 1 Pages: 79-84

    • DOI

      10.1538/expanim.63.79

    • NAID

      130003391606

    • ISSN
      0007-5124, 1341-1357, 1881-7122
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity.2013

    • Author(s)
      Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T
    • Journal Title

      Sci Rep.

      Volume: 3 Issue: 1 Pages: 3379-3379

    • DOI

      10.1038/srep03379

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of Sry knockout mouse using TALEN via oocyte injection.2013

    • Author(s)
      Kato T, Miyata K, Sonobe M, Yamashita S, Tamano M, Miura K, Kanai Y, Miyamoto S, Sakuma T, Yamamoto T, Inui M, Kikusui T, Asahara H, Takada S.
    • Journal Title

      Scientific reports

      Volume: 3 Issue: 1 Pages: 3136-3136

    • DOI

      10.1038/srep03136

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Vectors for TALEN- and CRISPR/Cas9-mediated genome editing2015

    • Author(s)
      Tetsushi Sakuma
    • Organizer
      第21回日本遺伝子治療学会学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2015-07-24 – 2015-07-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ゲノム編集の最前線と合成生物学への展開2015

    • Author(s)
      佐久間哲史
    • Organizer
      2015アジレント ゲノミクスフォーラム
    • Place of Presentation
      東京
    • Year and Date
      2015-06-16
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Front-line of genome editing technology for animal cell engineering2015

    • Author(s)
      Tetsushi Sakuma
    • Organizer
      The 24th Meeting of the European Society for Animal Cell Technology
    • Place of Presentation
      Barcelona, Spain
    • Year and Date
      2015-05-31 – 2015-06-03
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Platinum TALENおよびマルチガイドCRISPRシステムを用いたゲノム編集2015

    • Author(s)
      佐久間哲史
    • Organizer
      ゲノム編集マウスワークショップ
    • Place of Presentation
      つくば
    • Year and Date
      2015-03-17
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] PITCh systems: MMEJ-assisted gene knockin in human cells using TALENs and CRISPR/Cas92015

    • Author(s)
      Tetsushi Sakuma, Shota Nakade, Yuto Sakane, Ken-ichi T Suzuki, Takashi Yamamoto
    • Organizer
      CRISPR Precision Gene Editing Congress
    • Place of Presentation
      MA, USA
    • Year and Date
      2015-02-25 – 2015-02-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] MMEJ-mediated integration of donor DNA in cells and animals using TALENs and CRISPR/Cas92015

    • Author(s)
      Shota Nakade, Takuya Tsubota, Yuto Sakane, Satoshi Kume, Naoaki Sakamoto, Masanobu Obara, Takaaki Daimon, Hideki Sezutsu, Takashi Yamamoto, Tetsushi Sakuma, Ken-ichi T. Suzuki
    • Organizer
      Keystone Symposia: Precision Genome Engineering and Synthetic Biology
    • Place of Presentation
      MT, USA
    • Year and Date
      2015-01-11 – 2015-01-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Identification of Sterol Side Chain Reductase 2 (SSR2), a Key Enzyme in the Biosynthesis of Cholesterol As the Common Precursor of Toxic Steroidal Glycoalkaloids in Potato2015

    • Author(s)
      Kazuki Saito, Satoru Sawai, Kiyoshi Ohyama, Shuhei Yasumoto, Hikaru Seki, Tetsushi Sakuma, Takashi Yamamoto, Yumiko Takebayashi, Mikiko Kojima, Hitoshi Sakakibara, Toshio Aoki, Toshiya Muranaka, Naoyuki Umemoto
    • Organizer
      International Plant & Animal Genome XXIII
    • Place of Presentation
      CA, USA
    • Year and Date
      2015-01-10 – 2015-01-14
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ゲノム編集の基礎と動物における利用の現状2014

    • Author(s)
      佐久間哲史
    • Organizer
      平成26年度日本実験動物技術者協会 関西支部 秋季広島大会
    • Place of Presentation
      広島
    • Year and Date
      2014-11-29 – 2014-11-30
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Functional analysis of Oct60 in adult organ formation of Xenopus laevis2014

    • Author(s)
      Naoto Ito, Tetsushi Sakuma, Takashi Yamamoto, Tsutomu Kinoshita
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Gene expression and function of Oct91 during lateral line formation in Xenopus laevis2014

    • Author(s)
      Aya Amakawa, Tetsushi Sakuma, Takashi Yamamoto, Tsutomu Kinoshita
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] ゲノム編集技術をもちいた培養精子幹細胞株の遺伝子改変2014

    • Author(s)
      佐藤卓也, 佐久間哲史, 片桐久美子, 越後貫成美, 小倉淳郎, 山本 卓, 小川毅彦
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] ゲノム編集によるMMEJを利用した汎用的な遺伝子ノックイン法の確立2014

    • Author(s)
      中出翔太, 坂根祐人, 佐久間哲史, 小原政信, 鈴木賢一, 山本 卓
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] マウス胚性幹細胞におけるNanogプロモーターの確率的活性化が発現量の不均一性に影響する2014

    • Author(s)
      落合 博, 菅原武志, 佐久間哲史, 山本 卓
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] ネフロン前駆細胞特異的レポーター遺伝子を持つiPS細胞の樹立2014

    • Author(s)
      賀来祐介, 太口敦博, 佐久間哲史, 山本 卓, 西中村隆一
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 有用なモデル動物となり得る、イベリアトゲイモリを用いた遺伝子操作法の確立2014

    • Author(s)
      林 利憲, 茗荷あゆみ, 佐久間哲史, 亀井保博, 横山 仁, 山本 卓, 竹内 隆
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] グルタミン酸トランスポーターGLASTのヒトレアバリアントは網膜神経節細胞の脆弱性にin vivoで寄与する2014

    • Author(s)
      今橋里沙, 相田知海, 柳澤美智子, 佐久間哲史, 宇佐美貴子, 石久保春美, 山本 卓, 田中光一
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒト紡錘体形成チェックポイント欠損症における細胞増殖に共役した一次繊毛退縮制御の破綻による繊毛病発症機構2014

    • Author(s)
      宮本達雄, 細羽康介, 落合 博, Royba Ekaterina, 佐久間哲史, 山本 卓, 松浦伸也
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 核の凝縮機構の解析ー無細胞アポトーシス系を用いて2014

    • Author(s)
      刀祢重信, 杉本憲治, 網代廣三, 佐久間哲史, 山本 卓, 栗林 太
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 機能カセットノックインマウスのためのCRISPR/Casを用いたin vivoゲノム編集2014

    • Author(s)
      相田知海, Keiho Chiyo, 佐久間哲史, 宇佐美貴子, 石久保春美, 今橋里沙, 田中謙二, 山本 卓, 田中光一
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] ゲノム編集技術による簡便迅速かつ高効率な次世代染色体工学の開発2014

    • Author(s)
      野村 淳, 佐久間哲史, 神田暁史, 岸本恵子, 前田知花, 外丸祐介, 山本 卓, 内匠 透
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] マルチgRNAシステムを用いたCRISPR/Cas9によるゲノム編集2014

    • Author(s)
      佐久間哲史, 中出翔太, 西川綾美, 茶山一彰, 鈴木賢一, 山本 卓
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Transcription activator-like effector nucleases (TALENs)システムを用いて作製したphosphatase and tensin homolog (PTEN)ノックアウトメダカ2014

    • Author(s)
      松崎ゆり子, 佐久間哲史, 山本 卓, 佐谷秀行
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Front-line of genome engineering technologies2014

    • Author(s)
      Tetsushi Sakuma
    • Organizer
      The 27th Annual Meeting of Japanese Association for Animal Cell Technology
    • Place of Presentation
      北九州
    • Year and Date
      2014-11-11 – 2014-11-14
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Analysis of the effect on HBV life cycle by HBV genome editing using TALEN and CRISPR/Cas9 system2014

    • Author(s)
      Hiromi Abe, Tetsushi Sakuma, Masataka Tsuge, Nobuhiko Hiraga, Michio Imamura, Nelson Hayes, Hiroshi Aikata, Takashi Yamamoto, Kazuaki Chayama
    • Organizer
      AASLD The Liver Meeting 2014
    • Place of Presentation
      MA, USA
    • Year and Date
      2014-11-07 – 2014-11-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] Platinum TALENおよびCRISPR/Cas9を用いたゲノム編集2014

    • Author(s)
      佐久間哲史
    • Organizer
      第39回日本比較内分泌学会大会
    • Place of Presentation
      岡崎
    • Year and Date
      2014-11-07 – 2014-11-09
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Unliganded thyroid hormone receptor alpha regulates tissue sensitivity and gene repression as revealed by gene knockout in Xenopus tropicalis2014

    • Author(s)
      Jinyoung Choi, Ken-ichi T. Suzuki, Tetsushi Sakuma, Leena Shewade, Takashi Yamamoto, Daniel R. Buchholz
    • Organizer
      6th Great Lakes Nuclear Receptor Conference
    • Place of Presentation
      WI, USA
    • Year and Date
      2014-10-17 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by TALEN and CRISPR/Cas systems2014

    • Author(s)
      Akihiro Yasue, Silvia N. Mitsui, Takahito Watanabe, Tetsushi Sakuma, Seiichi Oyadomari, Takashi Yamamoto, Sumihare Noji, Taro Mito, Eiji Tanaka
    • Organizer
      X Meeting Spanish Society for Developmental Biology
    • Place of Presentation
      Madrid, Spain
    • Year and Date
      2014-10-13 – 2014-10-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] ゲノム編集の基礎と最新動向2014

    • Author(s)
      佐久間哲史
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] Platinum TALENおよびCRISPRマルチガイドシステムを用いたゲノム編集2014

    • Author(s)
      佐久間哲史, 中出翔太, 坂本尚昭, 鈴木賢一, 山本 卓
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] Duchenne型筋ジストロフィーiPS細胞の遺伝子修復とゲノムワイド変異解析2014

    • Author(s)
      李 紅梅, 藤本直子, 笹川典子, 白井紗矢, 大亀登紀子, 佐久間哲史, 田中道廣, 天野直己, 渡辺 亮, 櫻井英俊, 山本 卓, 山中伸弥, 堀田秋津
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] ゲノム編集技術による簡便迅速かつ高効率な次世代染色体工学の開発2014

    • Author(s)
      野村 淳, 佐久間哲史, 神田暁史, 岸本恵子, 前田知花, 外丸祐介, 山本 卓, 内匠 透
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] CRISPR/Casによるノックインマウス作製の効率化2014

    • Author(s)
      相田知海, Keiho Chiyo, 佐久間哲史, 宇佐美貴子, 石久保春美, 今橋 里沙, 田中謙二, 山本 卓, 田中光一
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] ゲノム編集技術を用いた遺伝子改変ラット作製の現状2014

    • Author(s)
      金子武人, 佐久間哲史, 山本 卓, 真下知士
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] CRISPR/Cas9 システムを用いた精巧な遺伝子導入技術の開発2014

    • Author(s)
      久野 悠, 佐久間哲史, 太田 聡, 王賀理恵, 岡本 仁, 山本 卓, 川原敦雄
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] ツメガエルにおける簡便なノックイン技術の確立:PITChシステム2014

    • Author(s)
      鈴木賢一, 坂根祐人, 佐久間哲史, 山本 卓
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] ゲノム編集のHIVへの応用2014

    • Author(s)
      蝦名博貴, 金村優香, 三沢尚子, 佐久間哲史, 小林朋子, 山本 卓, 小柳義夫
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒト培養細胞におけるMMEJを利用した汎用的な遺伝子ノックイン法の確立2014

    • Author(s)
      中出翔太, 佐久間哲史, 坂本尚昭, 鈴木賢一, 山本 卓
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] 一塩基置換導入法による高発がん性遺伝病の変異の特定2014

    • Author(s)
      落合 博, 宮本達雄, 佐久間哲史, 山本 卓, 松浦伸也
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] ゲノム編集技術をもちいた培養精子幹細胞株の遺伝子改変法の開発2014

    • Author(s)
      佐藤卓也, 佐久間哲史, 片桐久美子, 越後貫成美, 小倉淳郎, 山本 卓, 小川毅彦
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] 糸状菌型Platinum TALENの作製とイネいもち病菌(糸状菌)における高効率遺伝子改変2014

    • Author(s)
      荒添貴之, 小川哲央, 大里修一, 佐久間哲史, 山本 卓, 有江 力, 桑田 茂
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] TALENを用いた麹菌Aspergillus oryzaeにおけるゲノム編集2014

    • Author(s)
      水谷 治, 荒添貴之, 利田賢次, 林 梨咲, 大里修一, 佐久間哲史, 山本 卓, 桑田 茂, 山田 修
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] TALENを用いたジャガイモグリコアルカロイド生合成酵素遺伝子の破壊2014

    • Author(s)
      安本周平, 關 光, 澤井 学, 大山 清, 梅基直行, 佐久間哲史, 山本 卓, 斉藤和希, 村中俊哉
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] ミジンコ(Daphnia pulex)におけるPlatinum TALENを用いた標的遺伝子破壊法の確立2014

    • Author(s)
      蛭田千鶴江, 荻野由紀子, 佐久間哲史, 豊田賢治, 山本 卓, 井口泰泉
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] Increased or decreased expression of the ladybird bomeobox1 causes scoliosis in zebrafish2014

    • Author(s)
      Long Guo, Hiroshi Yamashita, Tetsushi Sakuma, Ikuyo Kou, Aki Takimoto, Takashi Yamamoto, Taiji Adachi, Yuji Hiraki, Shiro Ikegawa, Chisa Shukunami
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] TALENを用いたメダカの環境依存的性決定機構の解析2014

    • Author(s)
      竹中亨彰, 濱崎 心, 内村友哉, 長船奈津美, 高木久徳, 佐久間哲史, 山本 卓, 北野 健
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] アフリカツメガエルにおけるTALENを用いた標的遺伝子の改変2014

    • Author(s)
      坂根祐人, 佐久間哲史, 鈴木美有紀, 柏木啓子, 柏木昭彦, 坂本尚昭, 山本 卓, 鈴木賢一
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] In vivo monitoring of histone H3 lysine 9 acetylation using Mintbody transgenic Xenopus laevis during embryogenesis and tail regeneration2014

    • Author(s)
      鈴木美有紀, 坂根祐人, 佐久間哲史, 坂本尚昭, 山本 卓, 鈴木賢一
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] ゲノム編集技術を用いた鳥類性決定関連遺伝子の解析2014

    • Author(s)
      枡岡久子, 中川祐樹, 江崎 僚, 佐久間哲史, 黒岩麻里, 山本 卓, 堀内浩幸
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] TALENによる効率的な遺伝子破壊マウスの作製―体外受精により得られた凍結受精卵の使用と採卵に用いる雌マウスの週齢検討―2014

    • Author(s)
      中川佳子, 佐久間哲史, 中潟直己, 大村谷昌樹, 山本 卓
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] Platinum TALENによる高速・高効率なノックインマウス作製とヒトゲノム多様性の個体レベルでの機能解釈2014

    • Author(s)
      今橋里沙, 相田知海, 柳澤美智子, 佐久間哲史, 宇佐美貴子, 石久保春美, 山本 卓, 田中光一
    • Organizer
      第4 回ゲノム編集研究会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-06 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] 高活性型Platinum TALENを用いたゲノム編集2014

    • Author(s)
      佐久間哲史
    • Organizer
      第1回KBRPワークショップ
    • Place of Presentation
      熊本
    • Year and Date
      2014-10-03
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Unliganded thyroid hormone receptor alpha regulates gene repression and developmental timing as revealed by gene knockout in Xenopus tropicalis2014

    • Author(s)
      Jinyoung Choi, Ken-ichi Suzuki, Tetsushi Sakuma, Leena Shewade, Takashi Yamamoto, Daniel R. Buchholz
    • Organizer
      15th International Xenopus Conference
    • Place of Presentation
      CA, USA
    • Year and Date
      2014-08-24 – 2014-08-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Genome editing reveals a novel function of keratin in fin formation in X. laevis2014

    • Author(s)
      Ken-ichi T. Suzuki, Keiko Kashiwagi, Tetsushi Sakuma, Akihiko Kashiwagi, Makoto Mochii, Takashi Yamamoto
    • Organizer
      15th International Xenopus Conference
    • Place of Presentation
      CA, USA
    • Year and Date
      2014-08-24 – 2014-08-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of TALENs2014

    • Author(s)
      Yuto Sakane, Tetsushi Sakuma, Keiko Kashiwagi , Akihiko Kashiwagi, Takashi Yamamoto, Ken-ichi T Suzuki
    • Organizer
      15th International Xenopus Conference
    • Place of Presentation
      CA, USA
    • Year and Date
      2014-08-24 – 2014-08-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Stochastic promoter activation affects gene expression variability in murine embryonic stem cells2014

    • Author(s)
      Hiroshi Ochiai, Takeshi Sugawara, Ken-ichi Suzuki, Tetsushi Sakuma, Takashi Yamamoto
    • Organizer
      Cold Spring Harbor Laboratory Conference
    • Place of Presentation
      NY, USA
    • Year and Date
      2014-08-19 – 2014-08-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] Genome editing using Platinum TALENs2014

    • Author(s)
      Tetsushi Sakuma, Ken-ichi Suzuki, Takashi Yamamoto
    • Organizer
      FASEB Science Research Conference: Genome Engineering - Cutting-Edge Research and Applications
    • Place of Presentation
      Nassau, Bahamas
    • Year and Date
      2014-06-22 – 2014-06-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Ultra-rapid and highly efficient in vivo genome editing in mice2014

    • Author(s)
      Tomomi Aida, Tetsushi Sakuma, Takako Usami, Harumi Ishikubo, Risa Imahashi, Keiho Chiyo, Pawel Obrocki, Takashi Yamamoto, Kohichi Tanaka
    • Organizer
      FASEB Science Research Conference: Genome Engineering - Cutting-Edge Research and Applications
    • Place of Presentation
      Nassau, Bahamas
    • Year and Date
      2014-06-22 – 2014-06-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] ゲノム編集の原理と基礎知識2014

    • Author(s)
      佐久間哲史
    • Organizer
      第66回日本細胞生物学会大会
    • Place of Presentation
      奈良
    • Year and Date
      2014-06-11 – 2014-06-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Knockout of genes in the chordate Ciona intestinalis2014

    • Author(s)
      Nicholas Treen, Haruka Sasaki, Keita Yoshida, Akiko Hozumi, Tetsushi Sakuma, Takashi Yamamoto, Yasunori Sasakura
    • Organizer
      第47回日本発生生物学会
    • Place of Presentation
      愛知
    • Year and Date
      2014-05-27 – 2014-05-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] TALEN-mediated genome editing is very useful in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration2014

    • Author(s)
      Toshinori Hayashi, Kousuke Sakamoto, Tetsushi Sakuma, Ayumi Myouga, Naoki Yokotani, Takeshi Inoue, Eri Kawaguchi, Kiyokazu Agata, Takashi Yamamoto, Takashi Takeuchi
    • Organizer
      第47回日本発生生物学会
    • Place of Presentation
      愛知
    • Year and Date
      2014-05-27 – 2014-05-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] Targeted genome editing using Platinum TALENs2014

    • Author(s)
      Takashi Yamamoto, Kenichi Suzuki, Tetsushi Sakuma
    • Organizer
      第47回日本発生生物学会
    • Place of Presentation
      愛知
    • Year and Date
      2014-05-27 – 2014-05-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] A new strategy for gene knock-in in Xenopus laevis using Platinum TALENs2014

    • Author(s)
      Yuto Sakane, Tetsushi Sakuma, Miyuki Suzuki, Takashi Yamamoto, Ken-ichi Suzuki
    • Organizer
      第47回日本発生生物学会
    • Place of Presentation
      愛知
    • Year and Date
      2014-05-27 – 2014-05-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] Genome editing using Platinum TALENs2014

    • Author(s)
      Takashi Yamamoto, Ken-ichi Suzuki, Tetsushi Sakuma
    • Organizer
      第47回日本発生生物学会大会
    • Place of Presentation
      愛知
    • Related Report
      2013 Annual Research Report
  • [Presentation] Knockout of genes in the chordate Ciona intestinalis2014

    • Author(s)
      Nicholas Treen, Haruka Sasaki, Keita Yoshida, Akiko Hozumi, Tetsushi Sakuma, Takashi Yamamoto, Yasunori Sasakura
    • Organizer
      第47回日本発生生物学会大会
    • Place of Presentation
      愛知
    • Related Report
      2013 Annual Research Report
  • [Presentation] 人工ヌクレアーゼ TALEN によるトリテルペノイド生合成酵素遺伝子の破壊2014

    • Author(s)
      安本周平、關光、福島エリオデット、佐久間哲史、山本卓、村中俊哉
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山
    • Related Report
      2013 Annual Research Report
  • [Presentation] 植物の新奇転写抑制モチーフ -真核生物に保存された転写抑制機構の解析-2014

    • Author(s)
      伊藤岳、岡村僚太、佐久間哲史、山本卓、高橋陽介
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山
    • Related Report
      2013 Annual Research Report
  • [Presentation] PPR motif as a new DNA/RNA binding module for genome editing2014

    • Author(s)
      Yusuke Yagi, Tetsushi Sakuma, Takashi Yamamoto, Takahiro Nakamura
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山
    • Related Report
      2013 Annual Research Report
  • [Presentation] Platinum Gate TALEN system: Construction of highly-active Platinum TALENs2014

    • Author(s)
      Tetsushi Sakuma, Takashi Yamamoto
    • Organizer
      International Symposium on RNAi and Genome Editing Methods
    • Place of Presentation
      徳島
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using TALENs2014

    • Author(s)
      Ken-ichi T Suzuki, Yuto Sakane, Tetsushi Sakuma, Keiko Kashiwagi, Akihiko Kashiwagi, Takashi Yamamoto
    • Organizer
      International Symposium on RNAi and Genome Editing Methods
    • Place of Presentation
      徳島
    • Related Report
      2013 Annual Research Report
  • [Presentation] Highly efficient gene targeting in filamentous fungus Pyricularia oryzae by Platinum Gate TALEN system2014

    • Author(s)
      Takayuki Arazoe, Tetsuya Younomaru, Shuichi Ohsato, Tetsushi Sakuma, Takashi Yamamoto, Tsutomu Arie, Shigeru Kuwata
    • Organizer
      International Symposium on RNAi and Genome Editing Methods
    • Place of Presentation
      徳島
    • Related Report
      2013 Annual Research Report
  • [Presentation] Genome editing with TALENs and Crispr/Cas9 in the ascidian Ciona intestinalis2014

    • Author(s)
      Nicholas Treen, Keita Yoshida, Haruka Sasaki, Tetsushi Sakuma, Narudo Kawai, Takashi Yamamoto, Yasunori Sasakura
    • Organizer
      International Symposium on RNAi and Genome Editing Methods
    • Place of Presentation
      徳島
    • Related Report
      2013 Annual Research Report
  • [Presentation] A high efficient gene targeting in one-cell mouse embryos mediated by TALEN and CRISPR/Cas system2014

    • Author(s)
      Akihiro Yasue, Silvia Naomi Mitsui, Takahito Watanabe, Tetsushi Sakuma, Seiichi Oyadomari, Takashi Yamamoto, Sumihare Noji, Taro Mito, Eiji Tanaka
    • Organizer
      International Symposium on RNAi and Genome Editing Methods
    • Place of Presentation
      徳島
    • Related Report
      2013 Annual Research Report
  • [Presentation] Quantitative analysis of chromatin dynamics and gene expression in murine embryonic stem cells2014

    • Author(s)
      Hiroshi Ochiai, Takeshi Sugawara, Tetsushi Sakuma, Takashi Yamamoto
    • Organizer
      第2回クロマチン動態数理研究拠点 国際シンポジウム
    • Place of Presentation
      広島
    • Related Report
      2013 Annual Research Report
  • [Presentation] Front-line of genome editing technology in various animals2014

    • Author(s)
      Tetsushi Sakuma
    • Organizer
      2014 KALAS Winter Symposium
    • Place of Presentation
      韓国
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 高活性型Platinum TALENを用いた効率的なゲノム編集2013

    • Author(s)
      佐久間哲史、山本卓
    • Organizer
      第6回DNA鑑定学会大会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] 高活性型TALEN を用いたゲノム編集2013

    • Author(s)
      山本卓、鈴木賢一、相田知海、田中光一、佐久間哲史
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] Ascidian Genome Editing Using TALENs2013

    • Author(s)
      Nicholas Treen, Keita Yoshida, Tetsushi Sakuma, Haruka Sasaki, Narudo Kawai, Takashi Yamamoto, Yasunori Sasakura
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] A high efficient gene targeting in one-cell mouse embryos mediated by TALEN and CRISPR/Cas system2013

    • Author(s)
      Akihiro Yasue, Naomi Mitsui, Takahito Watanabe, Tetsushi Sakuma, Seiichi Oyadomari, Takashi Yamamoto, Sumihare Noji, Taro Mito, Eiji Tanaka
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] Highly efficient knockin mouse production by TALEN-mediated in vivo genome editing2013

    • Author(s)
      Tomomi Aida, Tetsushi Sakuma, Takako Usami, Harumi Ishikubo, Risa Imahashi, Takashi Yamamoto, Kohichi Tanaka
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] E-カドヘリンを発現しない上皮細胞の作製およびデスモソーム研究への応用2013

    • Author(s)
      鈴木信太郎、藤村基人、藤原美和子、佐久間哲史、山本卓
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] 分裂期キネシンKIF2Aを介した細胞増殖に共役した繊毛退縮機構2013

    • Author(s)
      宮本達雄、細羽康介、落合博、佐久間哲史、山本卓、松浦伸也
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] TALENを用いたツメガエル発生過程におけるサイトグロビンの機能解析2013

    • Author(s)
      中出翔太、鈴木賢一、佐久間哲史、重田美津紀、柏木昭彦、柏木啓子、山本卓、小原 政信
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] 人工多能性幹細胞における X 線に対する高感受性細胞死の p53依存的な誘導機構の解明2013

    • Author(s)
      香川晴信、河合秀彦、佐久間哲史、山本卓、塩谷文章、嶋本顕、田原栄俊
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] TALEN技術を用いたSry KOマウスの作出2013

    • Author(s)
      加藤朋子、宮田康平、園部未来、山下聡、玉野萌恵、宮本新吾、佐久間哲史、山本卓、乾雅史、菊水健史、浅原弘嗣、高田修治
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] 人工ヌクレアーゼTALENを用いたカタユウレイボヤの突然変異体系統の作製2013

    • Author(s)
      吉田 慶太、Treen Nicholas、保住暁子、佐久間哲史、山本卓、笹倉靖徳
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] FAST-idシステム:TALENによる変異導入細胞および染色体領域欠失細胞の濃縮システムの開発2013

    • Author(s)
      徳増大輔、佐久間哲史、林陽子、檜山英三、山本卓
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] ゲノム編集の新しいDNA/RNA結合モジュール、PPRモチーフ2013

    • Author(s)
      八木祐介、佐久間哲史、山本卓、中村崇裕
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] 動物におけるゲノム編集技術の最前線2013

    • Author(s)
      佐久間哲史
    • Organizer
      第31回九州実験動物研究会総会
    • Place of Presentation
      熊本
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 人工ヌクレアーゼによる一塩基編集法を利用したPCS(MVA)症候群の遺伝子間領域変異の同定2013

    • Author(s)
      松浦伸也、落合博、宮本達雄、金井昭教、細羽康介、佐久間哲史、浅見恵子、小川淳、渡辺輝浩、梶井正、山本卓
    • Organizer
      日本人類遺伝学会第58回大会
    • Place of Presentation
      宮城
    • Related Report
      2013 Annual Research Report
  • [Presentation] 高活性型Platinum TALENの効率的な作製システムの開発2013

    • Author(s)
      佐久間哲史、山本卓
    • Organizer
      第3回ゲノム編集研究会
    • Place of Presentation
      広島
    • Related Report
      2013 Annual Research Report
  • [Presentation] TALENの植物代謝工学研究への応用展開2013

    • Author(s)
      村中俊哉、安本周平、關光、福島エリオデット、佐久間哲史、山本卓
    • Organizer
      第3回ゲノム編集研究会
    • Place of Presentation
      広島
    • Related Report
      2013 Annual Research Report
  • [Presentation] DNA・RNA編集の新しい核酸結合モジュール, PPRモチーフ2013

    • Author(s)
      八木祐介、佐久間哲史、山本卓、中村崇裕
    • Organizer
      第3回ゲノム編集研究会
    • Place of Presentation
      広島
    • Related Report
      2013 Annual Research Report
  • [Presentation] 無尾両生類におけるゲノム編集2013

    • Author(s)
      鈴木賢一、坂根祐人、佐久間哲史、坂本尚昭、柏木啓子、柏木昭彦、山本卓
    • Organizer
      第3回ゲノム編集研究会
    • Place of Presentation
      広島
    • Related Report
      2013 Annual Research Report
  • [Presentation] TALENによるイモリのゲノム編集の可能性と, 再生研究への展開2013

    • Author(s)
      林利憲、坂本興亮、佐久間哲史、横谷直樹、井上武、川口恵里、阿形清和、山本卓、竹内隆
    • Organizer
      第3回ゲノム編集研究会
    • Place of Presentation
      広島
    • Related Report
      2013 Annual Research Report
  • [Presentation] TALENを用いた受精卵内遺伝子改変による高効率ノックインマウス作製2013

    • Author(s)
      相田知海、佐久間哲史、宇佐美貴子、石久保春美、今橋理沙、山本卓、田中光一
    • Organizer
      第3回ゲノム編集研究会
    • Place of Presentation
      広島
    • Related Report
      2013 Annual Research Report
  • [Presentation] 染色体工学技術とゲノム編集技術によるヒト化モデル動物の作製2013

    • Author(s)
      香月康宏、佐久間哲史、小林カオル、阿部智志、平林真澄、千葉寛、山本卓、押村光雄
    • Organizer
      第3回ゲノム編集研究会
    • Place of Presentation
      広島
    • Related Report
      2013 Annual Research Report
  • [Presentation] ゲノム編集技術を用いた霊長類疾患モデルの作製2013

    • Author(s)
      岸憲幸、佐藤賢哉、佐久間哲史、奥野弥佐子、岡野ジェイムズ洋尚、山本卓、佐々木えりか、岡野栄之
    • Organizer
      第3回ゲノム編集研究会
    • Place of Presentation
      広島
    • Related Report
      2013 Annual Research Report
  • [Presentation] TALEN RNAの受精卵へのマイクロインジェクションによるY連鎖遺伝子のノックアウトマウスの作製と解析2013

    • Author(s)
      加藤朋子、宮田康平、園部未来、山下聡、玉野萌恵、宮本新吾、佐久間哲史、山本卓、乾雅史、菊水健史、浅原弘嗣、高田修治
    • Organizer
      第3回ゲノム編集研究会
    • Place of Presentation
      広島
    • Related Report
      2013 Annual Research Report
  • [Presentation] TALEN, CRISPR/Casシステムを用いたマウス1細胞期胚における標的遺伝子破壊2013

    • Author(s)
      泰江章博、三井なおみ、渡辺崇人、佐久間哲史、親泊政一、山本卓、野地澄晴、三戸太郎、田中栄二
    • Organizer
      第3回ゲノム編集研究会
    • Place of Presentation
      広島
    • Related Report
      2013 Annual Research Report
  • [Presentation] TALENを用いたカタユウレイボヤ生殖細胞形成機構の解析2013

    • Author(s)
      白江-倉林麻貴、佐久間哲史、笹倉靖徳、中村輝、山本卓
    • Organizer
      第3回ゲノム編集研究会
    • Place of Presentation
      広島
    • Related Report
      2013 Annual Research Report
  • [Presentation] TALENを用いたゼブラフィッシュ母性効果遺伝子の機能解析2013

    • Author(s)
      黒川大輔、川原敦雄、佐久間哲史、山本卓、赤坂甲治、相澤慎一
    • Organizer
      第3回ゲノム編集研究会
    • Place of Presentation
      広島
    • Related Report
      2013 Annual Research Report
  • [Presentation] FAST-idシステム:TALENによる変異導入細胞および染色体領域欠失細胞の濃縮システムの開発2013

    • Author(s)
      徳増大輔、佐久間哲史、林陽子、檜山英三、坂本尚昭、山本卓
    • Organizer
      第3回ゲノム編集研究会
    • Place of Presentation
      広島
    • Related Report
      2013 Annual Research Report
  • [Presentation] 無尾両生類におけるTALENを用いた標的遺伝子の多重破壊の試み2013

    • Author(s)
      坂根祐人、佐久間哲史、坂本尚昭、柏木啓子、柏木昭彦、山本卓、鈴木賢一
    • Organizer
      第3回ゲノム編集研究会
    • Place of Presentation
      広島
    • Related Report
      2013 Annual Research Report
  • [Presentation] Identification of an extragenic mutation of BUB1B gene for PCS (MVA) syndrome and functional analysis using TAL effector nucleases2013

    • Author(s)
      T. Miyamoto, H. Ochiai, A. Kanai, K. Hosoba, S. Kume, T. Sakuma, T. Yamamoto, S. Matsuura
    • Organizer
      American Society of Human Genetics 2013 Annual Meeting
    • Place of Presentation
      アメリカ
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細胞増殖シグナル依存的な一次繊毛抑制過程におけるPLK1-KIF2A経路の役割2013

    • Author(s)
      宮本達雄、細羽康介、落合博、佐久間哲史、山本卓、松浦伸也
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
  • [Presentation] Platinum Gate TALEN: Establishment of highly-active TALEN construction system2013

    • Author(s)
      Tetsushi Sakuma, Takashi Yamamoto
    • Organizer
      Conference on Transposition and Genome Engineering 2013
    • Place of Presentation
      ハンガリー
    • Related Report
      2013 Annual Research Report
  • [Presentation] TALENsを利用した効率的なノックアウトマウスの作製について2013

    • Author(s)
      中川佳子、佐久間哲史、黒田和子、吉松麗加、竹田直樹、佐々木賢一、山本卓、大村谷昌樹
    • Organizer
      日本実験動物技術者協会第47回総会
    • Place of Presentation
      岡山
    • Related Report
      2013 Annual Research Report
  • [Book] Targeted Genome Editing Using Engineered Nucleases: ZFNs, TALENs, and the CRISPR/Cas9 System2015

    • Author(s)
      Sakuma T, Yamamoto T (Yamamoto T, ed.)
    • Total Pages
      205
    • Publisher
      Springer Japan
    • Related Report
      2014 Annual Research Report
  • [Book] 実験医学別冊「今すぐ始めるゲノム編集」2014

    • Author(s)
      佐久間哲史(山本卓 編)
    • Total Pages
      205
    • Publisher
      羊土社
    • Related Report
      2014 Annual Research Report
  • [Remarks] 広島大学 分子遺伝学研究室

    • URL

      http://www.mls.sci.hiroshima-u.ac.jp/smg/index.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 広島大学 分子遺伝学研究室

    • URL

      http://www.mls.sci.hiroshima-u.ac.jp/smg/index.html

    • Related Report
      2013 Annual Research Report
  • [Patent(Industrial Property Rights)] 核酸挿入用ベクター2013

    • Inventor(s)
      鈴木賢一、坂根祐人、佐久間哲史、山本卓
    • Industrial Property Rights Holder
      国立大学法人広島大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-230349
    • Filing Date
      2013-11-06
    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-09-12   Modified: 2019-07-29  

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