Development of CRISPR/Cas system enabling advanced genome editing on chromosome level

• Project/Area Number: 25890014
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: System genome science
• Research Institution: Hiroshima University
• Principal Investigator: SAKUMA TETSUSHI 広島大学, 理学(系)研究科(研究院), 特任助教 (90711143)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ゲノム編集 / CRISPR/Cas9 / ゲノム生物工学 / 遺伝子ノックイン / CRISPR/Cas

Outline of Final Research Achievements

We developed a vector system enabling advanced genome editing such as simultaneous disruption of multiple genes and large chromosomal deletion, and evaluated its applicability in cultured cells and mice. In cultured cells, multiple targeted mutagenesis for up to seven genes and chromosomal deletion were demonstrated. In addition, it was shown that simultaneous gene disruption at multiple gene loci was feasible in mouse embryos by single plasmid injection using an all-in-one vector carrying FokI-dCas9, which is known as a highly-specific variant of Cas9.

Research Products

• [Journal Article] Desmocollin-2 alone forms functional desmosomal plaques, with the plaque formation requiring the juxtamembrane region and plakophilins (2015)
  • Author(s): Fujiwara M, Nagatomo A, Tsuda M, Obata S, Sakuma T, Yamamoto T, Suzuki ST
  • Journal Title: J Biochem Volume: -
  • Peer Reviewed
• [Journal Article] Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9 (2015)
  • Author(s): Hara S, Tamano M, Yamashita S, Kato T, Saito T, Sakuma T, Yamamoto T, Inui M, Takada S
  • Journal Title: Sci Rep Volume: -
  • Peer Reviewed / Open Access
• [Journal Article] Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes (2015)
  • Author(s): Nakagawa Y, Sakuma T, Sakamoto T, Ohmuraya M, Nakagata N, Yamamoto T
  • Journal Title: BMC Biotechnol Volume: - Issue: 1 Pages: 33-33
  • DOI: 10.1186/s12896-015-0144-x
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cloning-free CRISPR/Cas system facilitates functional cassette knockin in mice (2015)
  • Author(s): Aida T, Chiyo K, Usami T, Ishikubo H, Imahashi R, Wada Y, Tanaka K, Sakuma T, Yamamoto T, Tanaka K
  • Journal Title: Genome Biol Volume: 16 Issue: 1 Pages: 87-87
  • DOI: 10.1186/s13059-015-0653-x
  • Peer Reviewed / Open Access
• [Journal Article] Homeolog-specific targeted mutagenesis in Xenopus laevis using TALENs (2015)
  • Author(s): Nakade S, Sakuma T, Sakane Y, Hara Y, Kurabayashi A, Kashiwagi K, Kashiwagi A, Yamamoto T, Obara M
  • Journal Title: In Vitro Cell Dev Biol Anim Volume: - Issue: 9 Pages: 879-884
  • DOI: 10.1007/s11626-015-9912-0
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine (2015)
  • Author(s): Kawai N, Ogura Y, Ikuta T, Saiga H, Hamada M, Sakuma T, Yamamoto T, Satoh N, Sasakura Y
  • Journal Title: Developmental Biology Volume: 印刷中 Issue: 1 Pages: 43-56
  • DOI: 10.1016/j.ydbio.2015.03.018
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A High Excision Potential of TALENs for Integrated DNA of HIV-Based Lentiviral Vector (2015)
  • Author(s): Ebina H, Kanemura Y, Misawa N, Sakuma T, Kobayashi T, Yamamoto T, Koyanagi Y
  • Journal Title: PLoS One Volume: 10 Issue: 3 Pages: e0120047-e0120047
  • DOI: 10.1371/journal.pone.0120047
  • NAID: 120005619759
  • Peer Reviewed / Open Access
• [Journal Article] Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish. (2015)
  • Author(s): Hisano Y, Sakuma T, Nakade S, Ohga R, Ota S, Okamoto H, Yamamoto T, Kawahara A.
  • Journal Title: Sci Rep. Volume: 5 Issue: 1 Pages: 8841-8841
  • DOI: 10.1038/srep08841
  • Peer Reviewed / Open Access
• [Journal Article] Tailor-made TALEN system for highly efficient targeted gene replacement in the rice blast fungus (2015)
  • Author(s): Arazoe T, Ogawa T, Miyoshi K, Yamato T, Ohsato S, Sakuma T, Yamamoto T, Arie T, Kuwata S
  • Journal Title: Biotechnology and Bioengineering Volume: - Issue: 7 Pages: 1335-1342
  • DOI: 10.1002/bit.25559
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The microtubule depolymerizing activity of a mitotic kinesin protein KIF2A drives primary cilia disassembly coupled with cell proliferation (2015)
  • Author(s): Miyamoto T, Hosoba K, Ochiai H, Royba E, Izumi H, Sakuma T, Yamamoto T, Matsuura S
  • Journal Title: Cell Reports Volume: 10 Issue: 5 Pages: 664-673
  • DOI: 10.1016/j.celrep.2015.01.003
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Unliganded thyroid hormone receptor alpha regulates developmental timing via gene repression in Xenopus tropicalis (2015)
  • Author(s): J. Choi, K. T. Suzuki, T. Sakuma, L. Shewade, T. Yamamoto and D. R. Buchholz
  • Journal Title: Endocrinology Volume: 156 Issue: 2 Pages: 735-44
  • DOI: 10.1210/en.2014-1554
  • Peer Reviewed / Open Access
• [Journal Article] Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient iPS Cells by TALEN and CRISPR-Cas9 (2015)
  • Author(s): Hongmei Lisa Li
  • Journal Title: Stem Cell Reports Volume: 4 Issue: 1 Pages: 143-154
  • DOI: 10.1016/j.stemcr.2014.10.013
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9 (2014)
  • Author(s): S. Nakade, T. Tsubota, Y. Sakane, S. Kume, N. Sakamoto, M. Obara, T. Daimon, H. Sezutsu, T. Yamamoto, T. Sakuma and K. T. Suzuki
  • Journal Title: Nat Commun Volume: 5 Issue: 1 Pages: 5560-5560
  • DOI: 10.1038/ncomms6560
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Stochastic promoter activation affects Nanog expression variability in mouse embryonic stem cells (2014)
  • Author(s): Ochiai, H., Sugawara, T., Sakuma, T., Yamamoto, T.
  • Journal Title: Scientific Reports Volume: 4 Issue: 1 Pages: 7125-7125
  • DOI: 10.1038/srep07125
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Targeted gene disruption by use of transcription activator-like effector nuclease (TALEN) in the water flea Daphnia pulex (2014)
  • Author(s): Chizue Hiruta, Yukiko Ogino, Tetsushi Sakuma, Kenji Toyota, Shinichi Miyagawa, Takashi Yamamoto, Taisen Iguchi
  • Journal Title: BMC Biotechnology Volume: 14 Issue: 1 Pages: 95-95
  • DOI: 10.1186/s12896-014-0095-7
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Simple knockout by electroporation of engineered endonucleases into intact rat embryos (2014)
  • Author(s): Kaneko T, Sakuma T, Yamamoto T, Mashimo T
  • Journal Title: Sci Rep Volume: 4 Issue: 1 Pages: 6382-6382
  • DOI: 10.1038/srep06382
  • NAID: 120005477670
  • Peer Reviewed / Open Access
• [Journal Article] Sterol side chain reductase 2 is a key enzyme in the biosynthesis of cholesterol, the common precursor of toxic steroidal glycoalkaloids in potato (2014)
  • Author(s): Sawai, S., Ohyama, K., Yasumoto, S., Seki, H., Sakuma, T., Yamamoto, T., Takebayashi, Y., Kojima, M., Sakakibara, H., Aoki, T., Muranaka, T., Saito, K., Umemoto, N.
  • Journal Title: The Plant Cell Volume: 26 Issue: 9 Pages: 3763-3774
  • DOI: 10.1105/tpc.114.130096
  • Peer Reviewed / Open Access
• [Journal Article] Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies (2014)
  • Author(s): Kazuki Y, Yakura Y, Abe S, Osaki M, Kajitani N, Kazuki K, Takehara S, Honma K, Suemori H, Yamazaki S, Sakuma T, Toki T, Shimizu R, Nakauchi H, Yamamoto T, Oshimura M
  • Journal Title: Scientific reports Volume: 4 Issue: 1 Pages: 6136-6136
  • DOI: 10.1038/srep06136
  • Peer Reviewed / Open Access
• [Journal Article] EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step. (2014)
  • Author(s): Ninagawa S, Okada T, Sumitomo Y, Kamiya Y, Kato K, Horimoto S, Ishikawa T, Takeda S, Sakuma T, Yamamoto T, Mori K
  • Journal Title: J Cell Biol. Volume: 206 Issue: 3 Pages: 347-356
  • DOI: 10.1083/jcb.201404075
  • NAID: 120005468381
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems (2014)
  • Author(s): A. Yasue, S. N. Mitsui, T. Watanabe, T. Sakuma, S. Oyadomari, T. Yamamoto, S. Noji, T. Mito and E. Tanaka
  • Journal Title: Sci Rep Volume: 4 Issue: 1 Pages: 5705-5705
  • DOI: 10.1038/srep05705
  • Peer Reviewed / Open Access
• [Journal Article] Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system (2014)
  • Author(s): Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T
  • Journal Title: Sci Rep Volume: 4 Issue: 1 Pages: 5400-5400
  • DOI: 10.1038/srep05400
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Application of oocyte cryopreservation technology in TALEN-mediated mouse genome editing (2014)
  • Author(s): Nakagawa Y, Sakuma T, Nakagata N, Yamasaki S, Takeda N, Ohmuraya M, Yamamoto T
  • Journal Title: Exp Anim Volume: -
  • NAID: 130004677827
  • Peer Reviewed
• [Journal Article] Germ cell mutations of the ascidian Ciona intestinalis with TALE nucleases. (2014)
  • Author(s): Yoshida K, Treen N, Hozumi A, Sakuma T, Yamamoto T, Sasakura Y.
  • Journal Title: Genesis Volume: in press Issue: 5 Pages: 431-439
  • DOI: 10.1002/dvg.22770
  • Peer Reviewed
• [Journal Article] FAST-id system for enrichment of cells with TALEN-induced mutations and large deletions (2014)
  • Author(s): Tokumasu D, Sakuma T, Hayashi Y, Hosoi S, Hiyama E, Yamamoto T
  • Journal Title: Genes to Cells Volume: 19 Issue: 5 Pages: 419-431
  • DOI: 10.1111/gtc.12142
  • Peer Reviewed / Open Access
• [Journal Article] TALEN-mediated single-base-pair editing identification of an intergenic mutation upstream of BUB1B as causative of PCS (MVA) syndrome. (2014)
  • Author(s): Ochiai H, Miyamoto T, Kanai A, Hosoba K, Sakuma T, Kudo Y, Asami K, Ogawa A, Watanabe A, Kajii T, Yamamoto T, Matsuura S.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 111(4) Issue: 4 Pages: 1461-6
  • DOI: 10.1073/pnas.1317008111
  • Peer Reviewed
• [Journal Article] Tissue-specific and ubiquitous gene knockouts in Ciona by electroporating TALENs provide new approaches to investigate gene functions (2014)
  • Author(s): Treen, N., Yoshida, K., Sakuma, T., Sasaki, H., Kawai, N., Yamamoto, T., Sasakura, Y.
  • Journal Title: Development Volume: 141 Issue: 2 Pages: 481-487
  • DOI: 10.1242/dev.099572
  • Peer Reviewed
• [Journal Article] Nuclease-mediated genome editing: At the front-line of functional genomics technology. (2014)
  • Author(s): Sakuma, T., and Woltjen, K.
  • Journal Title: Development, growth & differentiation Volume: 56 Issue: 1 Pages: 2-13
  • DOI: 10.1111/dgd.12111
  • Peer Reviewed
• [Journal Article] Versatile strategy for isolating TALEN-mediated knockout mutants in Caenorhabditis elegans. (2014)
  • Author(s): Sugi T, Sakuma T, Ohtani T and Yamamoto T
  • Journal Title: Dev Growth Differ Volume: 56 Issue: 1 Pages: 78-85
  • DOI: 10.1111/dgd.12108
  • Peer Reviewed
• [Journal Article] Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of transcription activator-like effector nucleases (2014)
  • Author(s): Sakane, Y., Sakuma, T., Kashiwagi, K., Kashiwagi, A., Yamamoto, T. and Suzuki, K. T.
  • Journal Title: Dev Growth Differ Volume: 56(1) Issue: 1 Pages: 108-114
  • DOI: 10.1111/dgd.12105
  • Peer Reviewed / Open Access
• [Journal Article] Targeted mutagenesis in sea urchin embryos using TALENs. (2014)
  • Author(s): Hosoi S, Sakuma T, Sakamoto N and Yamamoto T
  • Journal Title: Dev Growth Differ Volume: 56 Issue: 1 Pages: 92-97
  • DOI: 10.1111/dgd.12099
  • Peer Reviewed
• [Journal Article] Transcription activator-like effector nucleases efficiently disrupt the target gene in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration. (2014)
  • Author(s): Hayashi T, Sakamoto K, Sakuma T, Yokotani N, Inoue T, Kawaguchi E, Agata K, Yamamoto T, Takeuchi T
  • Journal Title: Development, Growth and Differentiation Volume: 56 Issue: 1 Pages: 115-121
  • DOI: 10.1111/dgd.12103
  • Peer Reviewed / Open Access
• [Journal Article] TALEN-induced gene knock out in Drosophila. (2014)
  • Author(s): Kondo T
  • Journal Title: Development Growth and Differentiation Volume: 56:1 Issue: 1 Pages: 86-91
  • DOI: 10.1111/dgd.12097
  • Peer Reviewed
• [Journal Article] Screening Methods to Identify TALEN-Mediated Knockout Mice (2014)
  • Author(s): Nakagawa Y, Yamamoto T, Suzuki KI, Araki K, Takeda N, Ohmuraya M, Sakuma T
  • Journal Title: Experimental Animals Volume: 63 Issue: 1 Pages: 79-84
  • DOI: 10.1538/expanim.63.79
  • NAID: 130003391606
  • ISSN: 0007-5124, 1341-1357, 1881-7122
  • Peer Reviewed
• [Journal Article] Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. (2013)
  • Author(s): Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T
  • Journal Title: Sci Rep. Volume: 3 Issue: 1 Pages: 3379-3379
  • DOI: 10.1038/srep03379
  • Peer Reviewed
• [Journal Article] Production of Sry knockout mouse using TALEN via oocyte injection. (2013)
  • Author(s): Kato T, Miyata K, Sonobe M, Yamashita S, Tamano M, Miura K, Kanai Y, Miyamoto S, Sakuma T, Yamamoto T, Inui M, Kikusui T, Asahara H, Takada S.
  • Journal Title: Scientific reports Volume: 3 Issue: 1 Pages: 3136-3136
  • DOI: 10.1038/srep03136
  • Peer Reviewed
• [Presentation] Vectors for TALEN- and CRISPR/Cas9-mediated genome editing (2015)
  • Author(s): Tetsushi Sakuma
  • Organizer: 第21回日本遺伝子治療学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2015-07-24 – 2015-07-26
  • Invited
• [Presentation] ゲノム編集の最前線と合成生物学への展開 (2015)
  • Author(s): 佐久間哲史
  • Organizer: 2015アジレント ゲノミクスフォーラム
  • Place of Presentation: 東京
  • Year and Date: 2015-06-16
  • Invited
• [Presentation] Front-line of genome editing technology for animal cell engineering (2015)
  • Author(s): Tetsushi Sakuma
  • Organizer: The 24th Meeting of the European Society for Animal Cell Technology
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2015-05-31 – 2015-06-03
  • Invited
• [Presentation] Platinum TALENおよびマルチガイドCRISPRシステムを用いたゲノム編集 (2015)
  • Author(s): 佐久間哲史
  • Organizer: ゲノム編集マウスワークショップ
  • Place of Presentation: つくば
  • Year and Date: 2015-03-17
  • Invited
• [Presentation] PITCh systems: MMEJ-assisted gene knockin in human cells using TALENs and CRISPR/Cas9 (2015)
  • Author(s): Tetsushi Sakuma, Shota Nakade, Yuto Sakane, Ken-ichi T Suzuki, Takashi Yamamoto
  • Organizer: CRISPR Precision Gene Editing Congress
  • Place of Presentation: MA, USA
  • Year and Date: 2015-02-25 – 2015-02-27
• [Presentation] MMEJ-mediated integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9 (2015)
  • Author(s): Shota Nakade, Takuya Tsubota, Yuto Sakane, Satoshi Kume, Naoaki Sakamoto, Masanobu Obara, Takaaki Daimon, Hideki Sezutsu, Takashi Yamamoto, Tetsushi Sakuma, Ken-ichi T. Suzuki
  • Organizer: Keystone Symposia: Precision Genome Engineering and Synthetic Biology
  • Place of Presentation: MT, USA
  • Year and Date: 2015-01-11 – 2015-01-16
• [Presentation] Identification of Sterol Side Chain Reductase 2 (SSR2), a Key Enzyme in the Biosynthesis of Cholesterol As the Common Precursor of Toxic Steroidal Glycoalkaloids in Potato (2015)
  • Author(s): Kazuki Saito, Satoru Sawai, Kiyoshi Ohyama, Shuhei Yasumoto, Hikaru Seki, Tetsushi Sakuma, Takashi Yamamoto, Yumiko Takebayashi, Mikiko Kojima, Hitoshi Sakakibara, Toshio Aoki, Toshiya Muranaka, Naoyuki Umemoto
  • Organizer: International Plant & Animal Genome XXIII
  • Place of Presentation: CA, USA
  • Year and Date: 2015-01-10 – 2015-01-14
  • Invited
• [Presentation] ゲノム編集の基礎と動物における利用の現状 (2014)
  • Author(s): 佐久間哲史
  • Organizer: 平成26年度日本実験動物技術者協会 関西支部 秋季広島大会
  • Place of Presentation: 広島
  • Year and Date: 2014-11-29 – 2014-11-30
  • Invited
• [Presentation] Functional analysis of Oct60 in adult organ formation of Xenopus laevis (2014)
  • Author(s): Naoto Ito, Tetsushi Sakuma, Takashi Yamamoto, Tsutomu Kinoshita
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Gene expression and function of Oct91 during lateral line formation in Xenopus laevis (2014)
  • Author(s): Aya Amakawa, Tetsushi Sakuma, Takashi Yamamoto, Tsutomu Kinoshita
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] ゲノム編集技術をもちいた培養精子幹細胞株の遺伝子改変 (2014)
  • Author(s): 佐藤卓也, 佐久間哲史, 片桐久美子, 越後貫成美, 小倉淳郎, 山本 卓, 小川毅彦
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] ゲノム編集によるMMEJを利用した汎用的な遺伝子ノックイン法の確立 (2014)
  • Author(s): 中出翔太, 坂根祐人, 佐久間哲史, 小原政信, 鈴木賢一, 山本 卓
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] マウス胚性幹細胞におけるNanogプロモーターの確率的活性化が発現量の不均一性に影響する (2014)
  • Author(s): 落合 博, 菅原武志, 佐久間哲史, 山本 卓
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] ネフロン前駆細胞特異的レポーター遺伝子を持つiPS細胞の樹立 (2014)
  • Author(s): 賀来祐介, 太口敦博, 佐久間哲史, 山本 卓, 西中村隆一
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 有用なモデル動物となり得る、イベリアトゲイモリを用いた遺伝子操作法の確立 (2014)
  • Author(s): 林 利憲, 茗荷あゆみ, 佐久間哲史, 亀井保博, 横山 仁, 山本 卓, 竹内 隆
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] グルタミン酸トランスポーターGLASTのヒトレアバリアントは網膜神経節細胞の脆弱性にin vivoで寄与する (2014)
  • Author(s): 今橋里沙, 相田知海, 柳澤美智子, 佐久間哲史, 宇佐美貴子, 石久保春美, 山本 卓, 田中光一
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] ヒト紡錘体形成チェックポイント欠損症における細胞増殖に共役した一次繊毛退縮制御の破綻による繊毛病発症機構 (2014)
  • Author(s): 宮本達雄, 細羽康介, 落合 博, Royba Ekaterina, 佐久間哲史, 山本 卓, 松浦伸也
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 核の凝縮機構の解析ー無細胞アポトーシス系を用いて (2014)
  • Author(s): 刀祢重信, 杉本憲治, 網代廣三, 佐久間哲史, 山本 卓, 栗林 太
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 機能カセットノックインマウスのためのCRISPR/Casを用いたin vivoゲノム編集 (2014)
  • Author(s): 相田知海, Keiho Chiyo, 佐久間哲史, 宇佐美貴子, 石久保春美, 今橋里沙, 田中謙二, 山本 卓, 田中光一
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] ゲノム編集技術による簡便迅速かつ高効率な次世代染色体工学の開発 (2014)
  • Author(s): 野村 淳, 佐久間哲史, 神田暁史, 岸本恵子, 前田知花, 外丸祐介, 山本 卓, 内匠 透
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] マルチgRNAシステムを用いたCRISPR/Cas9によるゲノム編集 (2014)
  • Author(s): 佐久間哲史, 中出翔太, 西川綾美, 茶山一彰, 鈴木賢一, 山本 卓
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Transcription activator-like effector nucleases (TALENs)システムを用いて作製したphosphatase and tensin homolog (PTEN)ノックアウトメダカ (2014)
  • Author(s): 松崎ゆり子, 佐久間哲史, 山本 卓, 佐谷秀行
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Front-line of genome engineering technologies (2014)
  • Author(s): Tetsushi Sakuma
  • Organizer: The 27th Annual Meeting of Japanese Association for Animal Cell Technology
  • Place of Presentation: 北九州
  • Year and Date: 2014-11-11 – 2014-11-14
  • Invited
• [Presentation] Analysis of the effect on HBV life cycle by HBV genome editing using TALEN and CRISPR/Cas9 system (2014)
  • Author(s): Hiromi Abe, Tetsushi Sakuma, Masataka Tsuge, Nobuhiko Hiraga, Michio Imamura, Nelson Hayes, Hiroshi Aikata, Takashi Yamamoto, Kazuaki Chayama
  • Organizer: AASLD The Liver Meeting 2014
  • Place of Presentation: MA, USA
  • Year and Date: 2014-11-07 – 2014-11-11
• [Presentation] Platinum TALENおよびCRISPR/Cas9を用いたゲノム編集 (2014)
  • Author(s): 佐久間哲史
  • Organizer: 第39回日本比較内分泌学会大会
  • Place of Presentation: 岡崎
  • Year and Date: 2014-11-07 – 2014-11-09
  • Invited
• [Presentation] Unliganded thyroid hormone receptor alpha regulates tissue sensitivity and gene repression as revealed by gene knockout in Xenopus tropicalis (2014)
  • Author(s): Jinyoung Choi, Ken-ichi T. Suzuki, Tetsushi Sakuma, Leena Shewade, Takashi Yamamoto, Daniel R. Buchholz
  • Organizer: 6th Great Lakes Nuclear Receptor Conference
  • Place of Presentation: WI, USA
  • Year and Date: 2014-10-17 – 2014-10-18
• [Presentation] Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by TALEN and CRISPR/Cas systems (2014)
  • Author(s): Akihiro Yasue, Silvia N. Mitsui, Takahito Watanabe, Tetsushi Sakuma, Seiichi Oyadomari, Takashi Yamamoto, Sumihare Noji, Taro Mito, Eiji Tanaka
  • Organizer: X Meeting Spanish Society for Developmental Biology
  • Place of Presentation: Madrid, Spain
  • Year and Date: 2014-10-13 – 2014-10-15
• [Presentation] ゲノム編集の基礎と最新動向 (2014)
  • Author(s): 佐久間哲史
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] Platinum TALENおよびCRISPRマルチガイドシステムを用いたゲノム編集 (2014)
  • Author(s): 佐久間哲史, 中出翔太, 坂本尚昭, 鈴木賢一, 山本 卓
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] Duchenne型筋ジストロフィーiPS細胞の遺伝子修復とゲノムワイド変異解析 (2014)
  • Author(s): 李 紅梅, 藤本直子, 笹川典子, 白井紗矢, 大亀登紀子, 佐久間哲史, 田中道廣, 天野直己, 渡辺 亮, 櫻井英俊, 山本 卓, 山中伸弥, 堀田秋津
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] ゲノム編集技術による簡便迅速かつ高効率な次世代染色体工学の開発 (2014)
  • Author(s): 野村 淳, 佐久間哲史, 神田暁史, 岸本恵子, 前田知花, 外丸祐介, 山本 卓, 内匠 透
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] CRISPR/Casによるノックインマウス作製の効率化 (2014)
  • Author(s): 相田知海, Keiho Chiyo, 佐久間哲史, 宇佐美貴子, 石久保春美, 今橋 里沙, 田中謙二, 山本 卓, 田中光一
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] ゲノム編集技術を用いた遺伝子改変ラット作製の現状 (2014)
  • Author(s): 金子武人, 佐久間哲史, 山本 卓, 真下知士
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] CRISPR/Cas9 システムを用いた精巧な遺伝子導入技術の開発 (2014)
  • Author(s): 久野 悠, 佐久間哲史, 太田 聡, 王賀理恵, 岡本 仁, 山本 卓, 川原敦雄
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] ツメガエルにおける簡便なノックイン技術の確立:PITChシステム (2014)
  • Author(s): 鈴木賢一, 坂根祐人, 佐久間哲史, 山本 卓
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] ゲノム編集のHIVへの応用 (2014)
  • Author(s): 蝦名博貴, 金村優香, 三沢尚子, 佐久間哲史, 小林朋子, 山本 卓, 小柳義夫
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] ヒト培養細胞におけるMMEJを利用した汎用的な遺伝子ノックイン法の確立 (2014)
  • Author(s): 中出翔太, 佐久間哲史, 坂本尚昭, 鈴木賢一, 山本 卓
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] 一塩基置換導入法による高発がん性遺伝病の変異の特定 (2014)
  • Author(s): 落合 博, 宮本達雄, 佐久間哲史, 山本 卓, 松浦伸也
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] ゲノム編集技術をもちいた培養精子幹細胞株の遺伝子改変法の開発 (2014)
  • Author(s): 佐藤卓也, 佐久間哲史, 片桐久美子, 越後貫成美, 小倉淳郎, 山本 卓, 小川毅彦
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] 糸状菌型Platinum TALENの作製とイネいもち病菌(糸状菌)における高効率遺伝子改変 (2014)
  • Author(s): 荒添貴之, 小川哲央, 大里修一, 佐久間哲史, 山本 卓, 有江 力, 桑田 茂
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] TALENを用いた麹菌Aspergillus oryzaeにおけるゲノム編集 (2014)
  • Author(s): 水谷 治, 荒添貴之, 利田賢次, 林 梨咲, 大里修一, 佐久間哲史, 山本 卓, 桑田 茂, 山田 修
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] TALENを用いたジャガイモグリコアルカロイド生合成酵素遺伝子の破壊 (2014)
  • Author(s): 安本周平, 關 光, 澤井 学, 大山 清, 梅基直行, 佐久間哲史, 山本 卓, 斉藤和希, 村中俊哉
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] ミジンコ（Daphnia pulex）におけるPlatinum TALENを用いた標的遺伝子破壊法の確立 (2014)
  • Author(s): 蛭田千鶴江, 荻野由紀子, 佐久間哲史, 豊田賢治, 山本 卓, 井口泰泉
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] Increased or decreased expression of the ladybird bomeobox1 causes scoliosis in zebrafish (2014)
  • Author(s): Long Guo, Hiroshi Yamashita, Tetsushi Sakuma, Ikuyo Kou, Aki Takimoto, Takashi Yamamoto, Taiji Adachi, Yuji Hiraki, Shiro Ikegawa, Chisa Shukunami
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] TALENを用いたメダカの環境依存的性決定機構の解析 (2014)
  • Author(s): 竹中亨彰, 濱崎 心, 内村友哉, 長船奈津美, 高木久徳, 佐久間哲史, 山本 卓, 北野 健
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] アフリカツメガエルにおけるTALENを用いた標的遺伝子の改変 (2014)
  • Author(s): 坂根祐人, 佐久間哲史, 鈴木美有紀, 柏木啓子, 柏木昭彦, 坂本尚昭, 山本 卓, 鈴木賢一
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] In vivo monitoring of histone H3 lysine 9 acetylation using Mintbody transgenic Xenopus laevis during embryogenesis and tail regeneration (2014)
  • Author(s): 鈴木美有紀, 坂根祐人, 佐久間哲史, 坂本尚昭, 山本 卓, 鈴木賢一
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] ゲノム編集技術を用いた鳥類性決定関連遺伝子の解析 (2014)
  • Author(s): 枡岡久子, 中川祐樹, 江崎 僚, 佐久間哲史, 黒岩麻里, 山本 卓, 堀内浩幸
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] TALENによる効率的な遺伝子破壊マウスの作製―体外受精により得られた凍結受精卵の使用と採卵に用いる雌マウスの週齢検討― (2014)
  • Author(s): 中川佳子, 佐久間哲史, 中潟直己, 大村谷昌樹, 山本 卓
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] Platinum TALENによる高速・高効率なノックインマウス作製とヒトゲノム多様性の個体レベルでの機能解釈 (2014)
  • Author(s): 今橋里沙, 相田知海, 柳澤美智子, 佐久間哲史, 宇佐美貴子, 石久保春美, 山本 卓, 田中光一
  • Organizer: 第4 回ゲノム編集研究会
  • Place of Presentation: 広島
  • Year and Date: 2014-10-06 – 2014-10-07
• [Presentation] 高活性型Platinum TALENを用いたゲノム編集 (2014)
  • Author(s): 佐久間哲史
  • Organizer: 第1回KBRPワークショップ
  • Place of Presentation: 熊本
  • Year and Date: 2014-10-03
  • Invited
• [Presentation] Unliganded thyroid hormone receptor alpha regulates gene repression and developmental timing as revealed by gene knockout in Xenopus tropicalis (2014)
  • Author(s): Jinyoung Choi, Ken-ichi Suzuki, Tetsushi Sakuma, Leena Shewade, Takashi Yamamoto, Daniel R. Buchholz
  • Organizer: 15th International Xenopus Conference
  • Place of Presentation: CA, USA
  • Year and Date: 2014-08-24 – 2014-08-28
• [Presentation] Genome editing reveals a novel function of keratin in fin formation in X. laevis (2014)
  • Author(s): Ken-ichi T. Suzuki, Keiko Kashiwagi, Tetsushi Sakuma, Akihiko Kashiwagi, Makoto Mochii, Takashi Yamamoto
  • Organizer: 15th International Xenopus Conference
  • Place of Presentation: CA, USA
  • Year and Date: 2014-08-24 – 2014-08-28
• [Presentation] Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of TALENs (2014)
  • Author(s): Yuto Sakane, Tetsushi Sakuma, Keiko Kashiwagi , Akihiko Kashiwagi, Takashi Yamamoto, Ken-ichi T Suzuki
  • Organizer: 15th International Xenopus Conference
  • Place of Presentation: CA, USA
  • Year and Date: 2014-08-24 – 2014-08-28
• [Presentation] Stochastic promoter activation affects gene expression variability in murine embryonic stem cells (2014)
  • Author(s): Hiroshi Ochiai, Takeshi Sugawara, Ken-ichi Suzuki, Tetsushi Sakuma, Takashi Yamamoto
  • Organizer: Cold Spring Harbor Laboratory Conference
  • Place of Presentation: NY, USA
  • Year and Date: 2014-08-19 – 2014-08-23
• [Presentation] Genome editing using Platinum TALENs (2014)
  • Author(s): Tetsushi Sakuma, Ken-ichi Suzuki, Takashi Yamamoto
  • Organizer: FASEB Science Research Conference: Genome Engineering - Cutting-Edge Research and Applications
  • Place of Presentation: Nassau, Bahamas
  • Year and Date: 2014-06-22 – 2014-06-27
• [Presentation] Ultra-rapid and highly efficient in vivo genome editing in mice (2014)
  • Author(s): Tomomi Aida, Tetsushi Sakuma, Takako Usami, Harumi Ishikubo, Risa Imahashi, Keiho Chiyo, Pawel Obrocki, Takashi Yamamoto, Kohichi Tanaka
  • Organizer: FASEB Science Research Conference: Genome Engineering - Cutting-Edge Research and Applications
  • Place of Presentation: Nassau, Bahamas
  • Year and Date: 2014-06-22 – 2014-06-27
• [Presentation] ゲノム編集の原理と基礎知識 (2014)
  • Author(s): 佐久間哲史
  • Organizer: 第66回日本細胞生物学会大会
  • Place of Presentation: 奈良
  • Year and Date: 2014-06-11 – 2014-06-13
  • Invited
• [Presentation] Knockout of genes in the chordate Ciona intestinalis (2014)
  • Author(s): Nicholas Treen, Haruka Sasaki, Keita Yoshida, Akiko Hozumi, Tetsushi Sakuma, Takashi Yamamoto, Yasunori Sasakura
  • Organizer: 第47回日本発生生物学会
  • Place of Presentation: 愛知
  • Year and Date: 2014-05-27 – 2014-05-30
• [Presentation] TALEN-mediated genome editing is very useful in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration (2014)
  • Author(s): Toshinori Hayashi, Kousuke Sakamoto, Tetsushi Sakuma, Ayumi Myouga, Naoki Yokotani, Takeshi Inoue, Eri Kawaguchi, Kiyokazu Agata, Takashi Yamamoto, Takashi Takeuchi
  • Organizer: 第47回日本発生生物学会
  • Place of Presentation: 愛知
  • Year and Date: 2014-05-27 – 2014-05-30
• [Presentation] Targeted genome editing using Platinum TALENs (2014)
  • Author(s): Takashi Yamamoto, Kenichi Suzuki, Tetsushi Sakuma
  • Organizer: 第47回日本発生生物学会
  • Place of Presentation: 愛知
  • Year and Date: 2014-05-27 – 2014-05-30
• [Presentation] A new strategy for gene knock-in in Xenopus laevis using Platinum TALENs (2014)
  • Author(s): Yuto Sakane, Tetsushi Sakuma, Miyuki Suzuki, Takashi Yamamoto, Ken-ichi Suzuki
  • Organizer: 第47回日本発生生物学会
  • Place of Presentation: 愛知
  • Year and Date: 2014-05-27 – 2014-05-30
• [Presentation] Genome editing using Platinum TALENs (2014)
  • Author(s): Takashi Yamamoto, Ken-ichi Suzuki, Tetsushi Sakuma
  • Organizer: 第47回日本発生生物学会大会
  • Place of Presentation: 愛知
• [Presentation] Knockout of genes in the chordate Ciona intestinalis (2014)
  • Author(s): Nicholas Treen, Haruka Sasaki, Keita Yoshida, Akiko Hozumi, Tetsushi Sakuma, Takashi Yamamoto, Yasunori Sasakura
  • Organizer: 第47回日本発生生物学会大会
  • Place of Presentation: 愛知
• [Presentation] 人工ヌクレアーゼ TALEN によるトリテルペノイド生合成酵素遺伝子の破壊 (2014)
  • Author(s): 安本周平、關光、福島エリオデット、佐久間哲史、山本卓、村中俊哉
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
• [Presentation] 植物の新奇転写抑制モチーフ －真核生物に保存された転写抑制機構の解析－ (2014)
  • Author(s): 伊藤岳、岡村僚太、佐久間哲史、山本卓、高橋陽介
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
• [Presentation] PPR motif as a new DNA/RNA binding module for genome editing (2014)
  • Author(s): Yusuke Yagi, Tetsushi Sakuma, Takashi Yamamoto, Takahiro Nakamura
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
• [Presentation] Platinum Gate TALEN system: Construction of highly-active Platinum TALENs (2014)
  • Author(s): Tetsushi Sakuma, Takashi Yamamoto
  • Organizer: International Symposium on RNAi and Genome Editing Methods
  • Place of Presentation: 徳島
  • Invited
• [Presentation] Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using TALENs (2014)
  • Author(s): Ken-ichi T Suzuki, Yuto Sakane, Tetsushi Sakuma, Keiko Kashiwagi, Akihiko Kashiwagi, Takashi Yamamoto
  • Organizer: International Symposium on RNAi and Genome Editing Methods
  • Place of Presentation: 徳島
• [Presentation] Highly efficient gene targeting in filamentous fungus Pyricularia oryzae by Platinum Gate TALEN system (2014)
  • Author(s): Takayuki Arazoe, Tetsuya Younomaru, Shuichi Ohsato, Tetsushi Sakuma, Takashi Yamamoto, Tsutomu Arie, Shigeru Kuwata
  • Organizer: International Symposium on RNAi and Genome Editing Methods
  • Place of Presentation: 徳島
• [Presentation] Genome editing with TALENs and Crispr/Cas9 in the ascidian Ciona intestinalis (2014)
  • Author(s): Nicholas Treen, Keita Yoshida, Haruka Sasaki, Tetsushi Sakuma, Narudo Kawai, Takashi Yamamoto, Yasunori Sasakura
  • Organizer: International Symposium on RNAi and Genome Editing Methods
  • Place of Presentation: 徳島
• [Presentation] A high efficient gene targeting in one-cell mouse embryos mediated by TALEN and CRISPR/Cas system (2014)
  • Author(s): Akihiro Yasue, Silvia Naomi Mitsui, Takahito Watanabe, Tetsushi Sakuma, Seiichi Oyadomari, Takashi Yamamoto, Sumihare Noji, Taro Mito, Eiji Tanaka
  • Organizer: International Symposium on RNAi and Genome Editing Methods
  • Place of Presentation: 徳島
• [Presentation] Quantitative analysis of chromatin dynamics and gene expression in murine embryonic stem cells (2014)
  • Author(s): Hiroshi Ochiai, Takeshi Sugawara, Tetsushi Sakuma, Takashi Yamamoto
  • Organizer: 第2回クロマチン動態数理研究拠点 国際シンポジウム
  • Place of Presentation: 広島
• [Presentation] Front-line of genome editing technology in various animals (2014)
  • Author(s): Tetsushi Sakuma
  • Organizer: 2014 KALAS Winter Symposium
  • Place of Presentation: 韓国
  • Invited
• [Presentation] 高活性型Platinum TALENを用いた効率的なゲノム編集 (2013)
  • Author(s): 佐久間哲史、山本卓
  • Organizer: 第6回DNA鑑定学会大会
  • Place of Presentation: 東京
• [Presentation] 高活性型TALEN を用いたゲノム編集 (2013)
  • Author(s): 山本卓、鈴木賢一、相田知海、田中光一、佐久間哲史
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Ascidian Genome Editing Using TALENs (2013)
  • Author(s): Nicholas Treen, Keita Yoshida, Tetsushi Sakuma, Haruka Sasaki, Narudo Kawai, Takashi Yamamoto, Yasunori Sasakura
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] A high efficient gene targeting in one-cell mouse embryos mediated by TALEN and CRISPR/Cas system (2013)
  • Author(s): Akihiro Yasue, Naomi Mitsui, Takahito Watanabe, Tetsushi Sakuma, Seiichi Oyadomari, Takashi Yamamoto, Sumihare Noji, Taro Mito, Eiji Tanaka
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Highly efficient knockin mouse production by TALEN-mediated in vivo genome editing (2013)
  • Author(s): Tomomi Aida, Tetsushi Sakuma, Takako Usami, Harumi Ishikubo, Risa Imahashi, Takashi Yamamoto, Kohichi Tanaka
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] E-カドヘリンを発現しない上皮細胞の作製およびデスモソーム研究への応用 (2013)
  • Author(s): 鈴木信太郎、藤村基人、藤原美和子、佐久間哲史、山本卓
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] 分裂期キネシンKIF2Aを介した細胞増殖に共役した繊毛退縮機構 (2013)
  • Author(s): 宮本達雄、細羽康介、落合博、佐久間哲史、山本卓、松浦伸也
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] TALENを用いたツメガエル発生過程におけるサイトグロビンの機能解析 (2013)
  • Author(s): 中出翔太、鈴木賢一、佐久間哲史、重田美津紀、柏木昭彦、柏木啓子、山本卓、小原 政信
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] 人工多能性幹細胞における X 線に対する高感受性細胞死の p53依存的な誘導機構の解明 (2013)
  • Author(s): 香川晴信、河合秀彦、佐久間哲史、山本卓、塩谷文章、嶋本顕、田原栄俊
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] TALEN技術を用いたSry KOマウスの作出 (2013)
  • Author(s): 加藤朋子、宮田康平、園部未来、山下聡、玉野萌恵、宮本新吾、佐久間哲史、山本卓、乾雅史、菊水健史、浅原弘嗣、高田修治
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] 人工ヌクレアーゼTALENを用いたカタユウレイボヤの突然変異体系統の作製 (2013)
  • Author(s): 吉田 慶太、Treen Nicholas、保住暁子、佐久間哲史、山本卓、笹倉靖徳
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] FAST-idシステム：TALENによる変異導入細胞および染色体領域欠失細胞の濃縮システムの開発 (2013)
  • Author(s): 徳増大輔、佐久間哲史、林陽子、檜山英三、山本卓
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] ゲノム編集の新しいDNA/RNA結合モジュール、PPRモチーフ (2013)
  • Author(s): 八木祐介、佐久間哲史、山本卓、中村崇裕
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] 動物におけるゲノム編集技術の最前線 (2013)
  • Author(s): 佐久間哲史
  • Organizer: 第31回九州実験動物研究会総会
  • Place of Presentation: 熊本
  • Invited
• [Presentation] 人工ヌクレアーゼによる一塩基編集法を利用したPCS（MVA）症候群の遺伝子間領域変異の同定 (2013)
  • Author(s): 松浦伸也、落合博、宮本達雄、金井昭教、細羽康介、佐久間哲史、浅見恵子、小川淳、渡辺輝浩、梶井正、山本卓
  • Organizer: 日本人類遺伝学会第58回大会
  • Place of Presentation: 宮城
• [Presentation] 高活性型Platinum TALENの効率的な作製システムの開発 (2013)
  • Author(s): 佐久間哲史、山本卓
  • Organizer: 第３回ゲノム編集研究会
  • Place of Presentation: 広島
• [Presentation] TALENの植物代謝工学研究への応用展開 (2013)
  • Author(s): 村中俊哉、安本周平、關光、福島エリオデット、佐久間哲史、山本卓
  • Organizer: 第３回ゲノム編集研究会
  • Place of Presentation: 広島
• [Presentation] DNA・RNA編集の新しい核酸結合モジュール, PPRモチーフ (2013)
  • Author(s): 八木祐介、佐久間哲史、山本卓、中村崇裕
  • Organizer: 第３回ゲノム編集研究会
  • Place of Presentation: 広島
• [Presentation] 無尾両生類におけるゲノム編集 (2013)
  • Author(s): 鈴木賢一、坂根祐人、佐久間哲史、坂本尚昭、柏木啓子、柏木昭彦、山本卓
  • Organizer: 第３回ゲノム編集研究会
  • Place of Presentation: 広島
• [Presentation] TALENによるイモリのゲノム編集の可能性と, 再生研究への展開 (2013)
  • Author(s): 林利憲、坂本興亮、佐久間哲史、横谷直樹、井上武、川口恵里、阿形清和、山本卓、竹内隆
  • Organizer: 第３回ゲノム編集研究会
  • Place of Presentation: 広島
• [Presentation] TALENを用いた受精卵内遺伝子改変による高効率ノックインマウス作製 (2013)
  • Author(s): 相田知海、佐久間哲史、宇佐美貴子、石久保春美、今橋理沙、山本卓、田中光一
  • Organizer: 第３回ゲノム編集研究会
  • Place of Presentation: 広島
• [Presentation] 染色体工学技術とゲノム編集技術によるヒト化モデル動物の作製 (2013)
  • Author(s): 香月康宏、佐久間哲史、小林カオル、阿部智志、平林真澄、千葉寛、山本卓、押村光雄
  • Organizer: 第３回ゲノム編集研究会
  • Place of Presentation: 広島
• [Presentation] ゲノム編集技術を用いた霊長類疾患モデルの作製 (2013)
  • Author(s): 岸憲幸、佐藤賢哉、佐久間哲史、奥野弥佐子、岡野ジェイムズ洋尚、山本卓、佐々木えりか、岡野栄之
  • Organizer: 第３回ゲノム編集研究会
  • Place of Presentation: 広島
• [Presentation] TALEN RNAの受精卵へのマイクロインジェクションによるY連鎖遺伝子のノックアウトマウスの作製と解析 (2013)
  • Author(s): 加藤朋子、宮田康平、園部未来、山下聡、玉野萌恵、宮本新吾、佐久間哲史、山本卓、乾雅史、菊水健史、浅原弘嗣、高田修治
  • Organizer: 第３回ゲノム編集研究会
  • Place of Presentation: 広島
• [Presentation] TALEN, CRISPR/Casシステムを用いたマウス１細胞期胚における標的遺伝子破壊 (2013)
  • Author(s): 泰江章博、三井なおみ、渡辺崇人、佐久間哲史、親泊政一、山本卓、野地澄晴、三戸太郎、田中栄二
  • Organizer: 第３回ゲノム編集研究会
  • Place of Presentation: 広島
• [Presentation] TALENを用いたカタユウレイボヤ生殖細胞形成機構の解析 (2013)
  • Author(s): 白江-倉林麻貴、佐久間哲史、笹倉靖徳、中村輝、山本卓
  • Organizer: 第３回ゲノム編集研究会
  • Place of Presentation: 広島
• [Presentation] TALENを用いたゼブラフィッシュ母性効果遺伝子の機能解析 (2013)
  • Author(s): 黒川大輔、川原敦雄、佐久間哲史、山本卓、赤坂甲治、相澤慎一
  • Organizer: 第３回ゲノム編集研究会
  • Place of Presentation: 広島
• [Presentation] FAST-idシステム：TALENによる変異導入細胞および染色体領域欠失細胞の濃縮システムの開発 (2013)
  • Author(s): 徳増大輔、佐久間哲史、林陽子、檜山英三、坂本尚昭、山本卓
  • Organizer: 第３回ゲノム編集研究会
  • Place of Presentation: 広島
• [Presentation] 無尾両生類におけるTALENを用いた標的遺伝子の多重破壊の試み (2013)
  • Author(s): 坂根祐人、佐久間哲史、坂本尚昭、柏木啓子、柏木昭彦、山本卓、鈴木賢一
  • Organizer: 第３回ゲノム編集研究会
  • Place of Presentation: 広島
• [Presentation] Identification of an extragenic mutation of BUB1B gene for PCS (MVA) syndrome and functional analysis using TAL effector nucleases (2013)
  • Author(s): T. Miyamoto, H. Ochiai, A. Kanai, K. Hosoba, S. Kume, T. Sakuma, T. Yamamoto, S. Matsuura
  • Organizer: American Society of Human Genetics 2013 Annual Meeting
  • Place of Presentation: アメリカ
• [Presentation] 細胞増殖シグナル依存的な一次繊毛抑制過程におけるPLK1-KIF2A経路の役割 (2013)
  • Author(s): 宮本達雄、細羽康介、落合博、佐久間哲史、山本卓、松浦伸也
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] Platinum Gate TALEN: Establishment of highly-active TALEN construction system (2013)
  • Author(s): Tetsushi Sakuma, Takashi Yamamoto
  • Organizer: Conference on Transposition and Genome Engineering 2013
  • Place of Presentation: ハンガリー
• [Presentation] TALENsを利用した効率的なノックアウトマウスの作製について (2013)
  • Author(s): 中川佳子、佐久間哲史、黒田和子、吉松麗加、竹田直樹、佐々木賢一、山本卓、大村谷昌樹
  • Organizer: 日本実験動物技術者協会第47回総会
  • Place of Presentation: 岡山
• [Book] Targeted Genome Editing Using Engineered Nucleases: ZFNs, TALENs, and the CRISPR/Cas9 System (2015)
  • Author(s): Sakuma T, Yamamoto T (Yamamoto T, ed.)
  • Total Pages: 205
  • Publisher: Springer Japan
• [Book] 実験医学別冊「今すぐ始めるゲノム編集」 (2014)
  • Author(s): 佐久間哲史（山本卓 編）
  • Total Pages: 205
  • Publisher: 羊土社
• [Remarks] 広島大学 分子遺伝学研究室
  • URL: http://www.mls.sci.hiroshima-u.ac.jp/smg/index.html
• [Remarks] 広島大学 分子遺伝学研究室
  • URL: http://www.mls.sci.hiroshima-u.ac.jp/smg/index.html
• [Patent(Industrial Property Rights)] 核酸挿入用ベクター (2013)
  • Inventor(s): 鈴木賢一、坂根祐人、佐久間哲史、山本卓
  • Industrial Property Rights Holder: 国立大学法人広島大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-230349
  • Filing Date: 2013-11-06