Mechanism of miRNA-mediated translational repression in plants

• Project/Area Number: 25891006
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: The University of Tokyo
• Principal Investigator: IWAKAWA Hiro-oki 東京大学, 分子細胞生物学研究所, 助教 (60710415)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: RNAサイレンシング / 核酸 / 蛋白質 / 発現制御

Outline of Final Research Achievements

Plant microRNAs (miRNAs) can mediate the cleavage of their target mRNAs as well as the repression of their translation. However, the molecular mechanism underlying miRNA-mediated translational repression remains unknown. Here, by using an in vitro system prepared from plant culture cells, we biochemically dissected the mechanisms of miRNA-mediated gene silencing.

Research Products

• [Journal Article] MicroRNAs block assembly of eIF4F translation initiation complex in Drosophila (2014)
  • Author(s): #Takashi Fukaya, #Hiro-oki Iwakawa, *Yukihide Tomari, (#同等貢献)
  • Journal Title: Molecular Cell Volume: 56 Issue: 1 Pages: 67-78
  • DOI: 10.1016/j.molcel.2014.09.004
  • Peer Reviewed
• [Journal Article] Molecular Insights into microRNA-Mediated Translational Repression in Plants. (2013)
  • Author(s): Iwakawa HO, *Tomari Y.
  • Journal Title: Mol Cell. Volume: 52(4) Issue: 4 Pages: 591-601
  • DOI: 10.1016/j.molcel.2013.10.033
  • Peer Reviewed
• [Journal Article] Arabidopsis ARGONAUTE7 selects miR390 through multiple checkpoints during RISC assembly. (2013)
  • Author(s): Endo Y, *Iwakawa HO, *Tomari Y.
  • Journal Title: EMBO Rep. Volume: 14(7) Issue: 7 Pages: 652-658
  • DOI: 10.1038/embor.2013.73
  • Peer Reviewed
• [Presentation] 植物におけるmicroRNAを介した翻訳抑制機構 (2014)
  • Author(s): 岩川 弘宙, 泊 幸秀
  • Organizer: RNAフロンティアミーティング 2014
  • Place of Presentation: 和歌山
  • Year and Date: 2014-09-16 – 2014-09-18
• [Presentation] 植物microRNAによる翻訳抑制機構 (2014)
  • Author(s): 岩川 弘宙, 泊 幸秀
  • Organizer: 第16回 RNA学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-07-23 – 2014-07-25
• [Presentation] The mechanistic bases for microRNA-mediated translational repression in plants (2013)
  • Author(s): 岩川 弘宙, 泊 幸秀
  • Organizer: 第86回 日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2013-09-11 – 2013-09-13