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Functional tuning mechanism of bitter taste receptors

Research Project

Project/Area Number 25891012
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Biophysics
Research InstitutionKyoto University

Principal Investigator

TSUTSUI Kei  京都大学, 霊長類研究所, 研究員 (40714209)

Project Period (FY) 2013-08-30 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords苦味受容体 / G蛋白質共役型受容体 / 感覚受容体 / GPCR
Outline of Final Research Achievements

In order to elucidate the mechanism by which sensory receptors tune their properties, we compared the ligand sensitivities of TAS2Rs of five species of NWM heterologously expressed in HEK293T cells by calcium imaging. We found that TAS2R1 and TAS2R4 orthologues differ in sensitivity among the NWM species for colchicine and camphor, respectively. These results raise the possibility that the different feeding ecologies of NWMs are at least in part associated to different sensitivities of their TAS2Rs. Moreover, we reconstructed ancestral receptors of the NWM TAS2R1 and TAS2R4 and measured the ligand sensitivity. The results revealed when the shifts in ligand sensitivity among species emerged in the course of molecular evolution and helped to identify candidate amino acid residues responsible for the shifts.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • Research Products

    (16 results)

All 2015 2014 2013 Other

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (13 results)

  • [Journal Article] Functional diversity of primate bitter taste receptors2015

    • Author(s)
      今井啓雄、筒井圭
    • Journal Title

      Hikaku seiri seikagaku(Comparative Physiology and Biochemistry)

      Volume: 32 Issue: 1 Pages: 24-29

    • DOI

      10.3330/hikakuseiriseika.32.24

    • NAID

      130005062337

    • ISSN
      0916-3786, 1881-9346
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Different phosphorylation rates among vertebrate cone visual pigments with different spectral sensitivities2013

    • Author(s)
      Kei Tsutsui
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 440 Pages: 630-634

    • NAID

      120005353493

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 霊長類苦味受容体の多様化2013

    • Author(s)
      今井啓雄
    • Journal Title

      生体の科学

      Volume: 64 Pages: 430-431

    • Related Report
      2013 Annual Research Report
  • [Presentation] 新世界ザルの苦味受容体応答の種間差2015

    • Author(s)
      筒井圭
    • Organizer
      第59回プリマーテス研究会
    • Place of Presentation
      犬山
    • Year and Date
      2015-01-31 – 2015-02-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] 新世界ザルにおけるTAS2R1およびTAS2R4の苦味応答の種間差2014

    • Author(s)
      筒井圭
    • Organizer
      生理学研究所研究会「細胞センサーの分子機構・相互関連・ネットワーク研究会」
    • Place of Presentation
      岡崎
    • Year and Date
      2014-12-04 – 2014-12-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] Interspecific Variation of Ligand Sensitivity and Evolution of Bitter Taste Receptors TAS2R1 and TAS2R4 in New World Monkeys2014

    • Author(s)
      筒井圭
    • Organizer
      12th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception
    • Place of Presentation
      福岡
    • Year and Date
      2014-11-02 – 2014-11-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] Interspecific Variation in Ligand Sensitivity of G-Protein-Coupled Bitter Taste Receptors in New World Monkeys2014

    • Author(s)
      筒井圭
    • Organizer
      16th International Conference on Retinal Proteins
    • Place of Presentation
      長浜
    • Year and Date
      2014-10-05 – 2014-10-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] Interspecific Variation of Ligand Sensitivity and Evolution of Bitter Taste Receptors TAS2R1 and TAS2R4 in New World Monkeys2014

    • Author(s)
      筒井圭
    • Organizer
      第52回日本生物物理学会
    • Place of Presentation
      札幌
    • Year and Date
      2014-09-24 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 錐体視物質グループ間におけるリン酸化効率の比較2014

    • Author(s)
      筒井圭
    • Organizer
      第18回日本光生物学協会年会
    • Place of Presentation
      大阪
    • Year and Date
      2014-08-22 – 2014-08-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] Functional Diversity of Bitter Taste Receptors in New World Monkeys2014

    • Author(s)
      筒井圭
    • Organizer
      25th Congress of the International Primatological Society
    • Place of Presentation
      ハノイ
    • Year and Date
      2014-08-11 – 2014-08-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Functional diversity of bitter taste receptors TAS2R1 and TAS2R4 in New World monkeys2013

    • Author(s)
      筒井 圭
    • Organizer
      The 11th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception
    • Place of Presentation
      九州大学(福岡市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Difference in sensitivity to sucralose and sucrose between human and Japanese monkey2013

    • Author(s)
      西 栄美子
    • Organizer
      The 11th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception
    • Place of Presentation
      九州大学(福岡市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Functional diversity of bitter taste receptors TAS2R1 and TAS2R4 in New World monkeys

    • Author(s)
      筒井 圭
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 全反射赤外分光法を用いたヒト苦味受容体の構造解析

    • Author(s)
      大橋 知明
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 錐体視物質グループ間におけるリン酸化効率の比較

    • Author(s)
      筒井 圭
    • Organizer
      分子研研究会 ロドプシン研究の故きを温ねて新しきを知る
    • Place of Presentation
      自然科学研究機構(岡崎市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 新世界ザルにおける苦味受容体TAS2R4のリガンド感受性の種間差

    • Author(s)
      筒井 圭
    • Organizer
      細胞センサーの分子機構・相互関連・ネットワーク研究会
    • Place of Presentation
      自然科学研究機構(岡崎市)
    • Related Report
      2013 Annual Research Report

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Published: 2013-09-12   Modified: 2019-07-29  

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