Generation and characterization of monoclonal antibodies against the extracellular regions of taste receptors

• Project/Area Number: 25891017
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: Okayama University
• Principal Investigator: YASUI Norihisa 岡山大学, 医歯(薬)学総合研究科, 助教 (90467514)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 抗体 / 味覚受容体 / Fab断片 / GPCR / 相互作用

Outline of Final Research Achievements

The sense of tastes by human and other animals depends on the recognition of tastant molecules by taste receptor proteins. In this project, we generated and characterized monoclonal antibodies against the extracellular regions of taste receptors. We have established the method to prepare the high-quality Fab fragments from IgGs and analyzed their interaction with recombinant proteins of the extracellular regions of taste receptors by using biochemical/biophysical techniques. We confirmed that the Fab fragments prepared here had the ability to bind the extracellular regions of taste receptors with high affinity.

Research Products

• [Journal Article] Directed network wiring identifies a key protein interaction in embryonic stem cell differentiation. (2014)
  • Author(s): Yasui, N., Findlay, G.M., Gish, G.D., Hsiung, M.S., Huang, J., Tucholska, M., Taylor, L., Smith, L., Boldridge W.C., Koide, A., Pawson, T, Koide, S.
  • Journal Title: Mol Cell Volume: 54 Issue: 6 Pages: 1034-1041
  • DOI: 10.1016/j.molcel.2014.05.002
  • Peer Reviewed / Open Access
• [Presentation] 人工タンパク質を用いた細胞内相互作用ネットワークの再構築によるマウスES細胞の分化に重要なタンパク質間相互作用の同定 (2014)
  • Author(s): 安井 典久, Greg M. Findlay, Gerald Gish, Marilyn S. Hsiung, Jin Huang, Monika Tucholska, Lorne Taylor, Louis Smith, W. Clifford Boldridge, 小出 明子, Tony Pawson, 小出 昌平
  • Organizer: 第37回分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-26
• [Presentation] 味覚受容体T1Rとカルモジュリンの相互作用 (2014)
  • Author(s): 伊藤 愛優美, 安井 典久，山下 敦子
  • Organizer: 第37回分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25
• [Presentation] 特定の配列モチーフに単一特異的に結合する人工タンパク質の作製とその利用によるタンパク質間相互作用の機能アノテーション (2014)
  • Author(s): 安井 典久, Greg M. Findlay, Gerald Gish, Marilyn S. Hsiung, Jin Huang, Monika Tucholska, Lorne Taylor, Louis Smith, W. Clifford Boldridge, 小出 明子, Tony Pawson, 小出 昌平
  • Organizer: 第14回日本蛋白質科学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-06-26
  • Invited