Functional analysis of quinone in the photosynthetic reaction center complex of green sulfur bacteria
Project/Area Number |
25891025
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Biophysics
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Research Institution | Ritsumeikan University |
Principal Investigator |
AZAI Chihiro 立命館大学, 生命科学部, 助教 (70706564)
|
Project Period (FY) |
2013-08-30 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 光合成反応中心 / 緑色硫黄細菌 / 広宿主域プラスミド / Hisタグ / 電子移動 / 過渡吸収 / キノン / 過渡吸収変化 |
Outline of Final Research Achievements |
A gene expression system by a broad host range plasmid was established in the green sulfur bacterium Chlorobaculum tepidum, which is a strictly anaerobic photosynthetic bacterium. Large-scale preparation of the homodimeric photosynthetic reaction center (RC) complex of green sulfur bacteria was easily achieved by over-expression of the His-tagged RC core-protein and its affinity purification. Electron transfer reactions in the green sulfur bacterial RC complex were observed by transient absorption spectroscopy from sub-nanosecond to millisecond time domains. The electron transfer pathway was affected by additives in the reaction solution; it was suggested that there is an unidentified cofactor serving as an alternate terminal electron acceptor of the normal iron-sulfur cluster in the green sulfur bacterial RC complex.
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Report
(3 results)
Research Products
(13 results)