| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Large crystals were obtained for the oxidized form of high-potential iron-sulfur protein. Neutron diffraction experiment was carried out at the beamline for life science (iBIX) of Japan Proton Accelerator Reseach complex J-PARC. A neutron diffraction data set was collected up to 1.1 angstrom resolution that is the highest resolution in the neutron diffraction data of proteins. After structure refinement, structures of hydrogen atoms were determined for ionizable amino acids and water molecules on the surface of the iron-sulfur protein. In addition, many deviations form ideal values were observed in the structure refinement by relaxing the restraints for bond angles and lengths of hydrogen atoms.
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