Studies on the mechanism for goadsporin resistance
| Project/Area Number |
25892008
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OZAKI Taro 東京大学, 農学生命科学研究科, 特任助教 (40709060)
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| Project Period (FY) |
2013-08-30 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 抗生物質 |
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| Research Abstract |
Goadsporin is an antibiotic which inhibits the growth of actinomycetes. As the molecular target of goadsporin was proposed to be unprecedented, it was expected that the characterization of the mechanism of goadsporin action would lead to the development of drugs which possessed novel mechanism of action. This study aimed to reveal the mechanism of goadsporin action. Ffh, a possible target of goadsporin was successfully expressed as a soluble protein in Escherichia coli. GodI, a goadsporin-resistant Ffh homologue was also obtained as a recombinant protein. Using recombinant Ffh and GodI, crystallization experiments were performed. As a result, microcrystal-like objects were obtained in some conditions.
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Report
(2 results)
Research Products
(4 results)
