Elucidation of the Novel Phosphorylase-Dependent Metabolisms of Glycosonjugates and Lipopolysaccharides

• Project/Area Number: 25892011
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: Niigata University
• Principal Investigator: CHIKU KAZUHIRO 新潟大学, 自然科学系, 研究員 (30711618)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 微生物酵素 / 糖質 / ホスホリラーゼ / 酵素 / 糖鎖 / 微生物

Outline of Final Research Achievements

Three novel β-mannoside phosphorylases (β-1,4-mannosyl-N-acetylglucosamine phosphorylase, β-1,2-mannbiose phosphorylase and β-1,2-oligomannan phosphorylase) were characterized as new member of glycoside hydrolase family 130 from Bacteroides thetaiotaomicron and Thermoanaerobacter sp. X-514. We further suggested that these β-mannoside phosphorylases play crunical roles in metabolisms of N-glycans or beta-1,2-oligomannan.

Research Products

• [Journal Article] 新規β-マンノシドホスホリラーゼの発見 (2015)
  • Author(s): 知久和寛，仁平高則，鈴木絵里香，西本完，北岡本光，中井博之，大坪研一
  • Journal Title: 応用糖質科学 Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Discovery of two β-1,2-Mannoside phosphorylases showing different chain-length specificities from Thermoanaerobacter sp. X-514 (2014)
  • Author(s): Kazuhiro Chiku, Takanori Nihira, Erika Suzuki, Mamoru Nishimoto, Motomitsu Kitaoka, Ken’ichi Ohtsubo, and Hiroyuki Nakai
  • Journal Title: PLoS One Volume: 9 Issue: 12 Pages: e114882-e114882
  • DOI: 10.1371/journal.pone.0114882
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] One Pot Enzymatic Production of Nigerose from Common Sugar Resources Employing Nigerose Phosphorylase (2014)
  • Author(s): Takanori Nihira, Futaba Miyajima, Kazuhiro Chiku, Mamoru Nishimoto, Motomitsu Kitaoka, Ken’ichi Ohtsubo, and Hiroyuki Nakai
  • Journal Title: Journal of Applied Glycoscience Volume: 61 Issue: 3 Pages: 75-80
  • DOI: 10.5458/jag.jag.JAG-2013_012
  • NAID: 130004481041
  • ISSN: 1344-7882, 1880-7291
  • Peer Reviewed
• [Journal Article] ニゲラン代謝に関わるClostridium phytofermentans由来の新規ホスホリラーゼの発見 (2014)
  • Author(s): 仁平高則，宮島双葉，知久和寛，大坪研一，中井博之，北岡本光
  • Journal Title: 応用糖質科学 Volume: 4 Pages: 147-153
  • Peer Reviewed
• [Presentation] 複合糖鎖の代謝に関与する新規βマンノシドホスホリラーゼの発見 (2014)
  • Author(s): 知久和寛，仁平高則，鈴木絵里香，西本完，北岡本光，中井博之，大坪研一
  • Organizer: 日本応用糖質科学会平成26年度大会(第63回)・応用糖質科学シンポジウム
  • Place of Presentation: 朱鷺メッセ 新潟コンベンションセンター（新潟）
  • Year and Date: 2014-09-26
• [Presentation] Saccharophagus degradans 2-40由来4-O-β-D-マンノシル-D-グルコースホスホリラーゼによる非還元性二糖の生産 (2014)
  • Author(s): 鈴木絵里香，知久和寛，仁平高則，西本完，北岡本光，中井博之，大坪研一
  • Organizer: 日本応用糖質科学会平成26年度大会(第63回)
  • Place of Presentation: 朱鷺メッセ 新潟コンベンションセンター（新潟）
  • Year and Date: 2014-09-24
• [Presentation] 新規1,2-β-オリゴマンナンホスホリラーゼ (2014)
  • Author(s): 仁平高則，知久和寛，鈴木絵里香，西本完，北岡本光，伏信進矢，中井博之，大坪研一
  • Organizer: 日本応用糖質科学会平成26年度大会(第63回)
  • Place of Presentation: 朱鷺メッセ 新潟コンベンションセンター（新潟）
  • Year and Date: 2014-09-24
• [Presentation] GH130に属する糖質加水分解酵素β-1,2-マンノシダーゼの発見 (2014)
  • Author(s): 知久和寛，仁平高則，鈴木絵里香，西本完，北岡本光，中井博之，大坪研一
  • Organizer: 日本応用糖質科学会平成26年度大会(第63回)
  • Place of Presentation: 朱鷺メッセ 新潟コンベンションセンター（新潟）
  • Year and Date: 2014-09-24