Identification of short and branched-chain aldehyde reductases and its application for unnatural biochemical production
Project/Area Number |
25892021
|
Research Category |
Grant-in-Aid for Research Activity Start-up
|
Allocation Type | Single-year Grants |
Research Field |
Applied microbiology
|
Research Institution | Yamaguchi University |
Principal Investigator |
|
Project Period (FY) |
2013-08-30 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 1,3-ブタンジオール / ブタノール / 3-ヒドロキシブチルアルデヒド / 大腸菌 / 合成代謝経路 / アルコール脱水素酵素 / 代謝工学 / 合成生物工学 |
Outline of Final Research Achievements |
The fermentation conditions including pH and overall oxygen transfer coefficient (kLa) were optimized to improve 1,3-butanediol production by metabolically engineered Escherichia coli. AdhP, EutG, and YqhD in E. coli were identified as alcohol dehydrogenases that catalyze the reduction of 3-hydroxybutyraldehyde to 1,3-butanediol. A CoA-dependent butanol synthetic pathway that functions under aerobic conditions was constructed in E. coli, by expanding the previously reported 1,3-butanediol synthetic pathway.
|
Report
(3 results)
Research Products
(12 results)
-
-
-
-
-
-
-
-
-
[Presentation] 組換え大腸菌による好気的ブタノール生成2014
Author(s)
片岡 尚也, Vangnai Alisa S., 加藤 純一, 藥師 寿治, 松下 一信.
Organizer
第66回日本生物工学会
Place of Presentation
札幌コンベンションセンター (北海道)
Year and Date
2014-09-09 – 2014-09-11
Related Report
-
-
-