Biochemical analysis of plant microRNA precursor processing

Research Project

Project/Area Number	25892025
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Applied molecular and cellular biology
Research Institution	Osaka Prefecture University
Principal Investigator	IWATA Yuji 大阪府立大学, 生命環境科学研究科(系), 助教 (80704965)
Project Period (FY)	2013-08-30 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000) Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	microRNA / シロイヌナズナ / DICER-LIKE 1
Outline of Final Research Achievements	MicroRNA (miRNA) is a small non-coding RNA molecule found in eukaryotes that functions in regulation of gene expression by binding to mRNA in a sequence-dependent manner and causing mRNA degradation and/or inhibition of translation. We analyzed molecular mechanism of miRNA precursor processing in the model plant Arabidopsis thaliana by using purified recombinant proteins expressed in insect cells. We gained insights into how efficient miRNA precursor processing is carried out by the RNaseIII family endoribonuclease Dicer-Like1 (DCL1) and its associated proteins.

Report

(3 results)