| Project/Area Number |
25893013
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2013-08-30 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | Cripto-1 / 大腸癌 / 個別化医療 / Ｃｒｉｐｔｏ－１ |
|---|
| Outline of Final Research Achievements |
Colorectal cancer specimens were obtained from 103 patients who underwent surgery at Tohoku University Hospital, from 2000 to 2006. Immunoreactivity for Cripto-1 was graded as positive, if >10% of cancer cells were stained. The association between Cripto-1 expression and the clinicopathological features was statistically analyzed. Cripto-1 was expressed in 49 cases. In clinicopathological features, Cripto-1 expression was positively associated with tumor size. Furthermore overall survival was significantly shorter in patients with positive expression of Cripto-1. In addition, Critpo-1 stable knockdown cell lines were generated using BLOCK-iT™ Pol II miR RNAi Expression Vector Kits for in vivo experiments. The Cripto-1 stable knockdown cell lines decreased cell proliferation, compared to the parental cells.
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