| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
The purpose of the present study is to examine miRNAs expression profiling on high metastatic potential cell lines and make integrated analysis of microRNA and mRNA with tumor metastasis in oral cancer.
To identify the possible targets of these miRNA, we performed bioinformatics analysis by TargetScan algorithm and integrated analysis across the data of human cancer cell lines and our mRNA microarray data to identify miRNAs whose expression correlated with the inverse expression of mRNA targets predicted in silico.
miRNA-205-5p was found down-regulated (more than -4 fold, P<0.001) in metastatic cell lines compared to non-metastatic cell lines. Results obtained from different searches by TargetScan algorithm predicted interferon regulatory factor 1 (IRF1) as a potential target for miRNA-205-5p. By qRT-PCR analyses, we confirmed up-regulation of IRF1 in metastatic cell lines. These data provided the evidence for an inverse correlation between the expression of miRNA-205-5p and IRF1.
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