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Physiological role of insulin granule docking in regulated exocytosis

Research Project

Project/Area Number 25893025
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionGunma University

Principal Investigator

MIZUNO kouichi  群馬大学, 生体調節研究所, 助教 (30321821)

Project Period (FY) 2013-08-30 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsインスリン分泌 / 糖尿病 / Rab27a / 全反射顕微鏡
Outline of Final Research Achievements

We directly observed insulin granule docking and fusion in pancreatic beta cell under total internal reflection fluorescence microscopy to figure out the physiological role of insulin granule docking in regulated exocytosis. This observation revealed that docked granules show lower fusion provability than that of undocked granules and they fuse with the dissociation of docking factor, granuphilin by recruiting a priming factor, Munc13-4 after fusion stimuli. These results suggest that docked granules might be trapped in a muted state, which opposites to a current model of regulated exocytosis that docked granules preferably fuse after fusion stimuli.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • Research Products

    (3 results)

All 2014 Other

All Presentation (3 results)

  • [Presentation] 生きたβ細胞で可視化することで明らかとなった、インスリン顆粒ドッキングとプライミングの分子基盤と機能的意義。2014

    • Author(s)
      水野広一、泉哲郎
    • Organizer
      日本糖尿病学会
    • Place of Presentation
      大阪
    • Year and Date
      2014-05-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Stimulus-induced Munc13-4 recruitment releases granuphilin to prime fusion-reluctant docked granules.

    • Author(s)
      Tetsuro Izumi and Kouichi Mizuno
    • Organizer
      APOCB congress and ASCB workshop
    • Place of Presentation
      Singapore
    • Related Report
      2013 Annual Research Report
  • [Presentation] 生きた膵β細胞で可視化することで明らかとなった、インスリン顆粒のドッキングとプライミングの分子基盤と機能的意義。

    • Author(s)
      水野広一、泉哲郎
    • Organizer
      日本糖尿病学会
    • Place of Presentation
      大阪
    • Related Report
      2013 Annual Research Report

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Published: 2013-09-12   Modified: 2019-07-29  

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