| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Previous studies have also shown that such sequelae affect neural stem cell and Schwann cell transplantation for nerves that have been damaged. Human induced pluripotent stem cells (hiPSCs) underwent monolayer culture on hiPSC maintenance medium on plastic dishes coated with Matrigel. Approximately 7 to 10 of these cells differentiated and were cultured further in Neural crest differentiation medium containing inhibitor. Markers of NCSCs; p75, Hnk1 were chosen as indicators of differentiation, while Sox2 was chosen as a marker for hiPSCs. After Immunocytochemistory and flow cytometry. It was found that the differentiated cells were negative for Sox2 and positive for p75 (65% ± 9%). Next, we performed that differentiated NCSCs transplanted to naked mouse sciatic nerve damage model. The specimen was not shown nerve degenerative findings.
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