The analysis of ASK1 pathway in brown adipocytes

• Project/Area Number: 25893036
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: HATTORI Kazuki 東京大学, 薬学研究科(研究院), 特任助教 (40708575)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 褐色脂肪 / ASK1 / Ucp1 / 熱産生 / 脂肪分化

Outline of Final Research Achievements

Brown adipose tissue is in the spotlight as a therapeutic target for some metabolic diseases. We revealed that the expression levels of specific subset of genes, which are known to be involved in thermogenesis, were suppressed in ASK1-deficient brown adipose tissue by DNA microarray analysis. Further analysis using primary culture of brown adipocytes elucidated that PKA-ASK1-p38 pathway is activated during the course of differentiation, which leads to gene induction, including Ucp1, and induces maturation of brown adipocytes.

Research Products

• [Presentation] 新規ケミカルプルダウン法を用いた褐色脂肪細胞におけるASK1複合体構成因子の同定 (2014)
  • Author(s): 曽我真弓、服部一輝、一條秀憲
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 褐色脂肪細胞の成熟化におけるASK1の機能解析 (2014)
  • Author(s): 服部一輝、一條秀憲
  • Organizer: 第35回日本肥満学会
  • Place of Presentation: 宮崎
  • Year and Date: 2014-10-24 – 2014-10-25
• [Presentation] 新規ケミカルプルダウン法を用いた褐色脂肪細胞におけるASK1複合体構成因子の同定 (2014)
  • Author(s): 曽我真弓、服部一輝、一條秀憲
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] ストレス応答性キナーゼASK1の脂肪組織における新規機能解析 (2014)
  • Author(s): 古谷昌太朗、服部一輝、一條秀憲
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] Cold stress induces cell death through the activation of ASK1-p38 pathway (2014)
  • Author(s): Hattori, K., Ishikawa, H., Naguro, I., Ichijo, H.
  • Organizer: Thiol-Based Redox Regulation & Signaling(Gordon Research Conference)
  • Place of Presentation: Girona, Spain
  • Year and Date: 2014-07-20 – 2014-07-25
• [Presentation] ASK1による褐色脂肪細胞の分化制御 (2014)
  • Author(s): 古谷昌太朗、服部一輝、一條秀憲
  • Organizer: 第23回日本Cell Death学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2014-07-18 – 2014-07-19
• [Presentation] Analog-sensitive kinaseを用いたプルダウン法による新規ASK1複合体構成因子の同定 (2014)
  • Author(s): 曽我真弓、服部一輝、丸山 剛、一條秀憲
  • Organizer: 第14回東京大学生命科学シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2014-04-26
• [Presentation] ASK1 regulates brown adipocyte differentiation (2014)
  • Author(s): Kazuki Hattori, Isao Naguro and Hidenori Ichijo
  • Organizer: Keystone Symposia
  • Place of Presentation: Vancouver, Canada
• [Presentation] 褐色脂肪細胞の分化におけるASK1の新規機能解析 (2013)
  • Author(s): 服部一輝、古谷昌太朗、名黒功、一條秀憲
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] The analysis of ASK1 function in blood glucose regulation (2013)
  • Author(s): Furutani, S., Hattori, K., Ichijo, H
  • Organizer: The 8th Japan-Korea conference on cellular signalling for young scientists
  • Place of Presentation: 九州大学(福岡県)
• [Presentation] ストレス応答性キナーゼASK1の血糖値制御に関わる機能解析 (2013)
  • Author(s): 古谷昌太朗、服部一輝、一條秀憲
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)