Functional analysis of SPAG4, a novel target of hypoxia-inducible factor (HIF) in the kidney

Research Project

Project/Area Number	25893045
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Kidney internal medicine
Research Institution	The University of Tokyo
Principal Investigator	SHOJI Kumi 東京大学, 医学部附属病院, 助教 (00439423)
Project Period (FY)	2013-08-30 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000) Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	低酸素 / 低酸素誘導因子 / 尿細管 / 腎臓内科学
Outline of Final Research Achievements	I assessed the expression pattern and the function of sperm-associated antigen 4 (SPAG4) which is a novel target of hypoxia-inducible factor, in the kidney. First, I revealed that hypoxia upregulates the expression of SPAG4 in the renal tubular cells both in vivo and in vitro. Next I generated SPAG4 conventional knockout mice using Cre/Lox P system for the first time. After ischemia-reperfusion injury, there were no significant differences in renal function and in renal histology between SPAG4 knockout mice and littermate control mice. The function of SPAG4 in the kidney remains to be solved.

Report

(3 results)