Development of a novel therapy for promoting liver regeneration and preventing postoperative therapy

• Project/Area Number: 25893052
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: The University of Tokyo
• Principal Investigator: INAGAKI Fuyuki 東京大学, 医学部附属病院, 登録研究医 (70529015)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 再生医療 / 肝切除 / 術後癒着 / 再生医学 / 消化器外科学 / 外科 / 移植・再生医療

Outline of Final Research Achievements

Repeated hepatectomy is widely accepted as one of the potentially curative treatments for primary and secondary liver neoplasms. But repeated hepatectomy has two major problems; postoperative adhesion and decreased regenerating ability of the remnant liver. We have already reported that the transplantation of mesothelial progenitor cells prevented postoperative adhesion and promoted liver regeneration in the mice practical partial hepatectomy model. In this study, we revealed the anti-adhesive effect of mesothelial cells transplantation in the miniature pig hepatectomy model. In addition, we showed that an allogeneic transplantation was as effective as a syngeneic transplantation in mice.

Research Products

• [Journal Article] Use of mouse liver mesothelial cells to prevent postoperative adhesion and promote liver regeneration after hepatectomy. (2015)
  • Author(s): Inagaki NF, Inagaki FF, Kokudo N, Miyajima A
  • Journal Title: Journal of hepatology Volume: 62 Issue: 5 Pages: 1141-7
  • DOI: 10.1016/j.jhep.2014.12.010
  • Peer Reviewed
• [Journal Article] Cell-based therapy for preventing postoperative adhesion and promoting regeneration after hepatectomy. (2015)
  • Author(s): Inagaki NF, Inagaki FF, Kokudo N, Miyajima A
  • Journal Title: Journal of Hepato-Billiary-Pancreatic Sciences Volume: 印刷中 Issue: 7 Pages: 524-530
  • DOI: 10.1002/jhbp.247
  • Peer Reviewed
• [Journal Article] Development of carboxymethyl cellulose nonwoven sheet as a novel hemostatic agent. (2015)
  • Author(s): Ohta S, Nishiyama T, Sakoda M, Machioka K, Fuke M, Ichimura S, Inagaki F, Shimizu A, Hasegawa K, Kokudo N, Kaneko M, Yatomi Y, Ito T.
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 印刷中 Issue: 6 Pages: 718-723
  • DOI: 10.1016/j.jbiosc.2014.10.026
  • NAID: 110009970764
  • Peer Reviewed
• [Journal Article] Nephronectin is upregulated in acute and chronic hepatitis and aggravates liver injury by recruiting CD4 positive cells (2013)
  • Author(s): Inagaki F., Tanaka M., Inagaki N., Yagai T., Sato Y., Sekiguchi K., Oyaizu N., Kokudo N., and Miyajima A
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 430 Issue: 2 Pages: 751-756
  • DOI: 10.1016/j.bbrc.2012.11.076
  • Peer Reviewed
• [Presentation] Study of the utility of the fetal liver mesothelial cells in the liver regeneration (2014)
  • Author(s): 稲垣奈都子, 稲垣冬樹, 國土典宏, 宮島篤
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] 肝中皮細胞を用いた細胞療法の開発 (2014)
  • Author(s): 稲垣奈都子, 稲垣冬樹, 國土典宏, 宮島篤
  • Organizer: 第21回肝細胞研究会
  • Place of Presentation: 東京
  • Year and Date: 2014-06-27 – 2014-06-28
• [Presentation] 胆管傷害および胆管癌におけるsterile alpha motif domain containg 5の発現解析 (2014)
  • Author(s): 谷貝知樹、松井理司、原田憲一、中沼安二、稲垣冬樹、西條栄子、宮島篤、田中稔
  • Organizer: 第21回肝細胞研究会
  • Place of Presentation: 東京
  • Year and Date: 2014-06-27 – 2014-06-28
• [Presentation] 臨床応用に向けた肝中皮細胞シートの有用性の検討 (2014)
  • Author(s): 稲垣奈都子, 稲垣冬樹, 國土典宏, 宮島篤
  • Organizer: 第50回日本肝臓学会総会
  • Place of Presentation: 東京
  • Year and Date: 2014-05-29 – 2014-05-30
• [Presentation] 他家移植における肝中皮細胞シートの有用性の検討 (2014)
  • Author(s): 稲垣奈都子、稲垣冬樹、國土典宏、宮島篤
  • Organizer: 第13回日本再生医療学会
  • Place of Presentation: 京都
• [Presentation] 肝中皮細胞シートを用いた次世代型再生医療 (2013)
  • Author(s): 稲垣奈都子、稲垣冬樹、國土典宏、宮島篤
  • Organizer: 第21回JDDW（日本消化器関連学会週間）
  • Place of Presentation: 品川
• [Patent(Industrial Property Rights)] iPS細胞からの中皮細胞の分化誘導系の確立 (2014)
  • Inventor(s): 稲垣冬樹、稲垣奈都子、宮島篤、國土典宏
  • Industrial Property Rights Holder: 稲垣冬樹、稲垣奈都子、宮島篤、國土典宏
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-05-25
  • Overseas
• [Patent(Industrial Property Rights)] 新規癒着防止療法および肝再生促進療法の開発ならびに新規肝切除後癒着動物モデルの確立 (2014)
  • Inventor(s): 稲垣奈都子、稲垣冬樹、國土典宏、宮島篤
  • Industrial Property Rights Holder: 稲垣奈都子、稲垣冬樹、國土典宏、宮島篤
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-01-22
  • Overseas