Elucidation of the expression pathway of adiponectin based on the integrated data analysis using epigenetic data

• Project/Area Number: 25893087
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Epidemiology and preventive medicine
• Research Institution: Nagoya University
• Principal Investigator: MASAHIRO Nakatochi 名古屋大学, 医学部附属病院, 病院助教 (10559983)
• Research Collaborator: YOKOTA Mitsuhiro 愛知学院大学, 歯学部, 教授 (50201851) ; MATSUBARA Tatsuaki 愛知学院大学, 歯学部, 教授 (30209598) ; ICHIHARA Sahoko 三重大学, 大学院地域イノベーション学研究科, 准教授 (20378326) ; YAMAMOTO Ken 久留米大学, 医学部, 教授 (60274528)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: エピゲノム / アディポネクチン / バイオインフォマティクス / DNAメチル化 / SNP / 生活習慣 / レジスチン / 生活習慣病

Outline of Final Research Achievements

Abnormal secretion of adiponectin and resistin is deeply involved in the development of lifestyle disease. These blood concentrations are known to be affected by the genetic factors. However, the variance of these concentrations explained by genes previously identified as associated genes with concentrations is very low.
In this study, we explored DNA methylation sites associated with the blood concentration of adiponectin and resistin using the data of blood concentration of them, SNP, and DNA methylation array for 192 nondiabetic men from a general population. As a result, we identified five sites for adiponectin and one site for resistin. Furthermore, DNA methylation level at the site associated with blood concentration of resistin was associated with SNP in vicinity of the site. These results suggest new insights into the regulation of blood concentration via effects on DNA methylation.

Research Products

• [Journal Article] A meta-analysis of genome-wide association studies for adiponectin levels in East Asians identifies a novel locus near WDR11-FGFR2. (2014)
  • Author(s): Wu Y, Gao H, Li H, Tabara Y, et al.
  • Journal Title: Hum Mol Genet. Volume: 23 Issue: 4 Pages: 1108-1119
  • DOI: 10.1093/hmg/ddt488
  • Peer Reviewed / Open Access
• [Journal Article] Identification of a glutamic acid repeat polymorphism of ALMS1 as a novel genetic risk marker for early-onset myocardial infarction by genome-wide linkage analysis. (2013)
  • Author(s): Ichihara S, Yamamoto K, Asano H, Nakatochi M, Sukegawa M, Ichihara G, Izawa H, Hirashiki A, Takatsu F, Umeda H, Iwase M, Inagaki H, Hirayama H, Sone T, Nishigaki K, Minatoguchi S, Cho MC, Jang Y, Kim HS, Yokota M. 他10名
  • Journal Title: Circ Cardiovasc Genet Volume: 6 Issue: 6 Pages: 569-578
  • DOI: 10.1161/circgenetics.111.000027
  • Peer Reviewed
• [Presentation] A meta-analysis of genome-wide association studies for adiponectin level in East Asians identifies a novel locus near WDR11-FGFR2 (2013)
  • Author(s): Wu Y, Gao H, Li H, Tabara Y, Nakatochi M, Chiu YF, Park EJ, Vadlamudi S, Fogarty M, Wen W, Shu XO, Shin C, Jee SH, Chuang LM, Miki T, Yokota M, Lin X, Mohlke KL, Tai ES, Asian Genetic Epidemiology Network (AGEN) Adiponectin Working Group
  • Organizer: American Society of Human Genetics 2013
  • Place of Presentation: Boston (America)
• [Presentation] Population based discovery of Tobacco-Smoking-Related differential DNA methylation (2013)
  • Author(s): Isomoto A, Kitajima H, Ichihara S, Nakatochi M, Matsubara T, Yokota M, Takayanagi R, Yamamoto K
  • Organizer: JSBi 2013
  • Place of Presentation: タワーホール船堀(東京都江戸川区)