| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Pre-osteoblastic cell line stably transfected with the GFP reporter gene driven by a fragment of type I collagen promoter was used in this study. Reporter-cells were cultured with small-molecules After 7 days, GFP fluorescence was measured. After 14 days of osteogenic induction, cells were subjected to ALP staining. In the result on screening of small-molecule library, three candidates were discovered. These three small molecules significantly enhanced osteoblastic differentiation of mouse bone marrow stem cells in vitro. The new bone formation in the calvarial defect was regenerated by candidate small-molecule in vivo. These results suggest that the cell-based screening method by the GFP and ALP staining assays could identify candidates for osteogenesis-targeting compounds in reliable manner in comparison to conventional methods, thus making it a promising tool for finding novel synthetic regulators of osteogenesis.
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