| Project/Area Number |
25893150
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
ZHANG Ying 山口大学, 医学(系)研究科(研究院), 助教 (10711260)
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| Research Collaborator |
KOBAYASHI Sei 山口大学, 大学院医学系研究科, 教授 (80225515)
KISHI Hiroko 山口大学, 大学院医学系研究科, 准教授 (40359899)
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| Project Period (FY) |
2013-08-30 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 血管攣縮 / 血管平滑筋 / 血管病 / 病的シグナル伝達 |
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| Outline of Final Research Achievements |
Vascular abnormal contraction, such as coronary artery and cerebrovascular vasospasm, is an important cause leading to myocardial infarction, ventricular arrhythmias, and sudden death. We have previously demonstrated that 「sphingosylphosphorylcholine (SPC)/Fyn/Rho-kinase」pathway involed in abnormal contraction of vascular smooth muscle (VSM). However, the molecular mechanisms by which Fyn functions in SPC-induced contraction remains unclear. Here, we identified the association of paxillin and the active Fyn by use of matrix assisted laser desorption ionization (MALDI) time-of-flight mass spectrometry. Immunostaining assay and immunoprecipitation assay further demonstrated that paxillin directly associated with constitutively active Fyn (CA-Fyn) and the activated Fyn by SPC. In addition, the siRNA-mediated knockdown of paxillin prevented SPC from inducing contraction.
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