| Project/Area Number |
25893151
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Parasitology (including sanitary zoology)
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| Research Institution | Yamaguchi University |
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Principal Investigator |
KUWATA Ryusei 山口大学, 理工学研究科, 研究員 (00711219)
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| Project Period (FY) |
2013-08-30 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | マダニ / 培養細胞 / ウイルス / 感染症 / 媒介節足動物 / 蚊 / 衛生害虫 |
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| Outline of Final Research Achievements |
In this study, I conducted to ① establish of tick cell line and ② survey for tick-borne viruses in Japan.
① I temporary cultured of the cells from the tissue of Amblyomma testudinarium in VP-12 medium with 15% FBS and 15% TPB. However, these cells were flowed because of the weak attachment to the bottom of culture dish. Now I tried to culture again with the same condition on ploy-L-lysine-coated dishes.
② We collected ticks at several sites of Japan, mainly Yamaguchi prefecture, by tick flagging technique or ticks parasitizing the wild animals. Collected ticks were identified and used for virus isolation or detection of viral gene. As a result, we successfully detected viral genes of undescribed species from RNA extraction of ticks collected in several place of Japan. Now we analyzed the characteristics of these virus species.
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