| Project/Area Number |
25893154
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Project Period (FY) |
2013-08-30 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 悪性腫瘍 / 口腔癌 / プロテオーム / タンパク質 / 生化学 / 分子生物学 |
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| Outline of Final Research Achievements |
We have performed proteomic differential display analysis for the expression of intracellular proteins in the regressive murine fibrosarcoma cell clone QR-32 and the progressive malignant tumor cell clone QRsP-11, derived from QR-32, by means of the combination of two-dimensional gel electrophoresis (2-DE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). We found several protein spots whose expression was different between QRsPs and identified those proteins, and calreticulin precursor (CALR), one of them, was over-expressed in the progressive tumor cell. Our RT-PCR results indicated that m-RNA level of CALR was up-regulated in oral squamous cell carsinomas (OSCCs). To Study the function of CALR in OSCC, we established a stable CALR-knockdown cell line. We tested colony forming ability of the knockdown cell line and there was a marked decrease in colony formation when CRT expression was depleted. These results indicated CALR might play an oncogenic role in OSCCs.
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