DC-Dependent efficient prevention of spontaneous neuroblastoma in MYCN transgenic mice
| Project/Area Number |
25893169
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatric surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2013-08-30 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 神経芽腫 / 腫瘍医学 / 小児外科学 / 免疫学 |
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| Outline of Final Research Achievements |
Cultivation of lineage-negative hematopoietic progenitors from bone marrow of the mice of the same strain of MYCN transgenic mice in an optimized cytokine cocktail led to production of a large amount and high-purity dendritic cells.
Importantly, subcutaneous injection of the DCs pulsed with their tumor lysate almost completely prevents the tumor formation and much improved their survival in these mice. The depletion study of the effector cells suggested that the DC-therapy is dependent to CD4-positive cells.These results suggest that DC-based vaccine may possibly be a potential therapeutic to prevent neuroblastoma in clinical setting.
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Report
(3 results)
Research Products
(1 results)
