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The role of microRNA in diabetes associated vascular dysfunction

Research Project

Project/Area Number 25893192
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionKagoshima University

Principal Investigator

MUNEKAZU Yamakuchi  鹿児島大学, 医歯(薬)学総合研究科, 准教授 (20325814)

Research Collaborator HASHIGUCHI Teruto  鹿児島大学, 大学院医歯学総合研究科, 教授 (70250917)
SHIMIZU Toshiaki  鹿児島大学, 大学院医歯学総合研究科, 助教 (50468055)
OYAMA Yoko  鹿児島大学, 医学部・歯学部付属病院, 特任助教 (20583470)
TAKENOUCHI Kazunori  鹿児島大学, 医学部・歯学部付属病院, 医員 (30646758)
Project Period (FY) 2013-08-30 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsマイクロRNA / 血小板 / 血管内皮細胞 / 糖尿病 / エクソゾーム / microRNA
Research Abstract

Diabetes is one of the major public health problem. Dysfunction of endothelial cells are involved in formation of the pathogenesis of this vascular diseases associated with diabetes. Our hypothesis is that cell to cell communication between endothelial cells and platelets would play a pivotal role in diabetic vascular complications. microRNAs (miRNAs) is a non-coding miRNAs that regulates protein synthesis post-transcriptionally. We used miRNAs as key players to organize a series of vascular events under hyperglycemia. We have discovered that miR-503 was induced by hypoxia in cultured endothelial cells and that miR-503 regulates phosphorylation of Akt as well as cyclin D. We also observed that miR-503 is released by wrapping in exosomes under hypoxic condition. In the future studies, we will focus on the downstream target of Akt regulated by miR-503 in endothelial cells and clarify the mechanisms by which endothelial cells transfer the information to platelets.

Report

(2 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (13 results)

All 2014 2013 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (5 results) Book (3 results) Remarks (1 results)

  • [Journal Article] CHARGE Consortium Hemostatic Factor Working Group. Genome-wide association study for circulating tissue plasminogen activator levels and functional follow-up implicates endothelial STXBP5 and STX22014

    • Author(s)
      Huang J, Huffman JE, Yamakuchi M, Trompet S,Lowenstein CJ, Strachan DP, O'Donnell CJ
    • Journal Title

      Arterioscler Thromb Vasc Biol

      Volume: 4(5) Pages: 1093-101

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Post- marketing surveillance of thrombomodulin alfa, a novel treatment of disseminated intravascular coagulation - Safety and efficacy in 1,032 patients with hematologic malignancy2014

    • Author(s)
      Hashiguchi T, Yamakuchi M
    • Journal Title

      Thrombosis Research

      Volume: 133 Pages: 364-370

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Upregulation of non-βCell-derived Vascular Endothelial Growth Factor A Increases Small Clusters of Insulin-producing Cells in the Pancreas2014

    • Author(s)
      Takenouchi K, Shrestha B, Yamakuchi M, Yoshinaga N, Arimura N, Kawaguchi H, Nagasato T, Feil R, Kawahara K, Sakamoto T, Maruyama I, Hashiguchi T
    • Journal Title

      Exp C lin Endocrinol Diabetes

      Volume: 122(5) Pages: 308-15

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Saturated fatty acid palmitate induces extracellular release of histone H3: a possible mechanistic basis for high-fat diet-induced inflammation and thrombosis2013

    • Author(s)
      Shrestha C, Ito T, Kawahara K, Shrestha B, Yamakuchi M, Hashiguchi T, Maruyama I
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 437(4) Pages: 573-8

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] アディポネクチンは新規炎症性アディポサイトカインHMGB1の分泌をJNK経由で阻害する2014

    • Author(s)
      清水利昭、竹之内和則、山口宗一、橋口照人
    • Organizer
      第59回日本臨床検査医学会九州地方会第25回日本臨床化学会合同プログラム
    • Place of Presentation
      熊本市医師会館
    • Year and Date
      2014-03-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Loop -Mediated Isothermal Amplification (LAMP)法を用いたClostridium difficile toxinの検討2014

    • Author(s)
      郡山豊泰、森明日華、古城剛、福山竜子、松下昌風、山口宗一、橋口照人
    • Organizer
      第59回日本臨床検査医学会九州地方会第25回日本臨床化学会合同プログラム
    • Place of Presentation
      熊本市医師会館
    • Year and Date
      2014-03-15
    • Related Report
      2013 Final Research Report
  • [Presentation] マイクロRNAによる直腸がん細胞の低酸素応答制御2013

    • Author(s)
      山口宗一、清水利昭、竹ノ内和則、大山陽子、郡山豊康、橋口照人
    • Organizer
      第60回日本臨床検査医学会学術集会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] New adipocytokine HMGB1 inhibits insulin signaling in adipocyte and adiponectin suppresses this effect2013

    • Author(s)
      Toshiaki Shimizu, Munekazu Yamakuchi, Shingo Yamada, Masanori Nakata, Teruto Hashiguchi, and Ikuro Maruyama
    • Organizer
      The Merinoff world Congress
    • Place of Presentation
      New York, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] マイクロRNAによる直腸がん細胞の低酸素応答制御2013

    • Author(s)
      山口宗一、清水利昭、竹ノ内和則、大山陽子、郡山豊康、橋口照人
    • Organizer
      日本臨床検査医学会学術集会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Annual Research Report
  • [Book] Iron Regulatory Protein 1 / HIF2α軸―鉄代謝調節メカニズムの新展開―臨床化学会2014

    • Author(s)
      橋口照人、Binita Shrestha、伊藤隆史、山口宗一
    • Related Report
      2013 Final Research Report
  • [Book] 臨床化学2014

    • Author(s)
      山口宗一
    • Total Pages
      9
    • Publisher
      日本臨床化学会
    • Related Report
      2013 Annual Research Report
  • [Book] デオキシチミジンキナーゼ臨床検査ガイド2013~20142013

    • Author(s)
      山口宗一
    • Total Pages
      1128
    • Publisher
      文光堂
    • Related Report
      2013 Final Research Report
  • [Remarks] ホームページ

    • URL

      http://www2.kufm.kagoshima-u.ac.jp/~mdio/list/j/j1/index.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2013-09-12   Modified: 2016-11-25  

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