| Project/Area Number |
25893216
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
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| Project Period (FY) |
2013-08-30 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 細胞外マトリックス / 関節破壊 / 滑膜 / 軟骨 / マトリックスメタロプロテアーゼ / 炎症性サイトカイン |
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| Research Abstract |
In the present study, we examined the effects of inflammatory cytokines on synovial fibroblasts and chondrocytes in vitro. Tumor necrosis factor-alpha (TNF-alpha) enhanced matrix metalloproteinase-13 (MMP-13) mRNA expression in chondrocytes. Pre-treatment with high molecular weight hyaluronic acid suppressed TNF-alpha-mediated enhancement of MMP-13.
On the other hand, interleukin-17 (IL-17) stimulates MMP-3 mRNA expression in synovial sarcoma cells. Immunofluorescent staining in synovial fibroblasts derived from human temporomandibular joints (TMJ) revealed that expression of IL-17 receptor (IL-17RA) that was primarily localized on the cell surface, whereas no immunofluorescent staining was seen in chondroyctes. Thesse results suggest that the main target of IL-17 in TMJ is synovial tissue.
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