| Project/Area Number |
25893217
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Periodontology
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
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| Project Period (FY) |
2013-08-30 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | Streptococcus sanguinis / LPS / ICAM-1 / 感染性心内膜炎 / 歯周病原細菌 |
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| Outline of Final Research Achievements |
A. actinomycetemcomitans LPS stimulation induced an increase in expression of ICAM-1 on the surface of RAW 264.7 cells, suggesting that ICAM-1 is related to cell-to-bacteria aggregation. Therefore, we examined whether pili adhere to cells via ICAM-1 on the surface of macrophages using a QCM technique. The frequency was decreased by approximately 2000 Hz in S. sanguinis SK36 pili-deficient-mutant, while that was decreased by approximately 14000 Hz in S. sanguinis SK36. These results suggest that pili on the surface of S. sanguinis bind to ICAM-1 on the surface of the cell clumps. Our findings also indicate that LPS enhances not only cell-to-cell, but also bacteria-cell aggregation via ICAM-1 on the surface of macrophages. Furthermore, there is a possibility that cell-to-bacteria interaction enhances cell-to-cell aggregation, suggesting that pili and ICAM-1 are involved in infective endocarditis caused by S. sanguinis via interaction of these molecules.
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