| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
The discrimination of normal and pathological cells is attractive from a clinical viewpoint. Tumor cells might have higher temperatures than normal ones as a result of enhanced metabolic activities. In addition, tumorous extracellur pH is slightly lower than that of normal tissue. Therefore, We developed poly(N-isopropylacrylamide)-based temperature-responsive fluorescence polymer probe, and pH-responsive fluorescence polymer probe. The cellular uptake of temperature-responsive fluorescence polymer probe can be controlled within only 1℃. pH-Responsive fluorescence polymer probe exhibited a specific intracellular uptake only in weak acidic conditions. The ability for temperature-responsive and acidic environment selective intracellular uptake opens up the visualization of selective tumors at the cellular level.
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