| Project/Area Number |
25893240
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Showa University |
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Principal Investigator |
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| Project Period (FY) |
2013-08-30 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 唾液腺 / 幹細胞 / 再生 / 口腔乾燥症 / BrdU / 長期保持細胞（LRC） |
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| Outline of Final Research Achievements |
As an analysis of the factors that is specifically expressed fetal salivaly gland, from head and neck frozen tissue sections of embryonic day 12 old mice, two areas of the oral mucosa and salivary gland were collected using laser microdissection. In addition, the CD133-positive cells from adult submandibular glands were collected by flow-cytometory as salivary gland adult stem cells. And we analyzed embryonic stage salivary gland and salivary gland adult stem cells gene expression profiles using RNA sequence. Using in situ hybridization for high transcription factor of both population, it was confirmed localization in adult submandibular gland and fetal salivaly gland.
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