Identification of enhanced salivary gland stem cell regenerative capacity factor and its application to treatment of disease

• Project/Area Number: 25893240
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Morphological basic dentistry
• Research Institution: Showa University
• Principal Investigator: JUNICHI Tanaka 昭和大学, 歯学部, 助教 (40710166)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 唾液腺 / 幹細胞 / 再生 / 口腔乾燥症 / BrdU / 長期保持細胞（LRC）

Outline of Final Research Achievements

As an analysis of the factors that is specifically expressed fetal salivaly gland, from head and neck frozen tissue sections of embryonic day 12 old mice, two areas of the oral mucosa and salivary gland were collected using laser microdissection. In addition, the CD133-positive cells from adult submandibular glands were collected by flow-cytometory as salivary gland adult stem cells. And we analyzed embryonic stage salivary gland and salivary gland adult stem cells gene expression profiles using RNA sequence. Using in situ hybridization for high transcription factor of both population, it was confirmed localization in adult submandibular gland and fetal salivaly gland.

Research Products

• [Journal Article] ANGPTL4 regulates the metastatic potential of oral squamous cell carcinoma. (2015)
  • Author(s): Tanaka J, Irie; T, Yamamoto G, Yasuhara R, Isobe T, Hokazono C, Tachikawa T, Kohno Y, Mishima K.
  • Journal Title: J Oral Pathol Med. Volume: 44(2) Issue: 2 Pages: 126-33
  • DOI: 10.1111/jop.12212
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Potential role of hematopoietic pre-B-cell leukemia transcription factor-interacting protein in oral carcinogenesis. (2015)
  • Author(s): Okada S, Irié T, Tanaka J, Yasuhara R, Yamamoto G, Isobe T, Hokazono C, Tachikawa T, Kohno Y, Mishima K.
  • Journal Title: J Oral Pathol Med. Volume: 44(2) Issue: 2 Pages: 115-25
  • DOI: 10.1111/jop.12210
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Zinc transporter SLC39A10/ZIP10 controls humoral immunity by modulating B-cell receptor signal strength. (2014)
  • Author(s): Hojyo S., T. Miyai, H. Fujishiro, M. Kawamura, T. Yasuda, A. Hijikata, B-H Bin, T. Irie, J. Tanaka, T. Atsumi, M. Murakami, M. Nakayama, O. Ohara, S. Himeno, H. Yoshida, H. Koseki, T. Ikawa, K. Mishima, and T. Fukada.
  • Journal Title: Proc Natl Acad Sci USA Volume: 111 Issue: 32 Pages: 11786-11791
  • DOI: 10.1073/pnas.1323557111
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The junctional epithelium originates from the odontogenic epithelium of an erupted tooth. (2014)
  • Author(s): Sara Yajima-Himuro, Masamitsu Oshima, Gou Yamamoto, Miho Ogawa, Madoka Furuya, Junichi Tanaka, Kousuke Nishii, Kenji Mishima, Tetsuhiko Tachikawa, Takashi Tsuji, Matsuo Yamamoto
  • Journal Title: Scientific Reports Volume: 4(4867) Issue: 1 Pages: 1-7
  • DOI: 10.1038/srep04867
  • Peer Reviewed / Open Access
• [Presentation] マウス唾液腺における幹細胞の同定とそのcharacterization (2015)
  • Author(s): 田中準一、馬渕 洋、安原理佳､ 入江太朗、深田俊幸、福島美和子、河野葉子、美島健二
  • Organizer: 第14回再生医療学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2015-03-21
• [Presentation] マウス唾液腺細胞におけるCD133陽性細胞の機能解析
  • Author(s): 田中準一、安原理佳、入江太朗、秋山知恵、河野葉子、美島健二
  • Organizer: 再生医療学会
  • Place of Presentation: 京都