| Project/Area Number |
25893241
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Prosthodontics/ Dental materials science and
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| Research Institution | Showa University |
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Principal Investigator |
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| Project Period (FY) |
2013-08-30 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | Porphyromonas gingivalis / periodontitis / protease / P.gingivalis / p.gingivalis / OPG / gingipain |
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| Outline of Final Research Achievements |
Periodontitis is a choronic inflammatory disease accompanied by alveolar bone resorption by osteoclasts. Porphyromonas gingivalis, one of the major pathogens of periodontitis, produces cysteine proteases called gingipains, that are clasified into Rgp and Kgp based on their cleavage site specificities. We previously found that Kgp degrades osteoprotegerin (OPG: 56kDa), an osteoclastogenesis-inhibitory factor, into an N-terminal fragment (37kDa) and inactivates OPG. In this study, The principal investigator tried to determine the specific cleavage sites in OPG cleaved by Kgp and prepare mutant OPG proteins resistant to the cleavage by Kgp. Unfortunately, a mutant OPG protein resistant to Kgp has not been obtained.
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