The roles of Rho family GTPases in inflammatory bone destruction

• Project/Area Number: 25893243
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Periodontology
• Research Institution: Showa University
• Principal Investigator: AIZAWA Ryo 昭和大学, 歯学部, 助教 (80710673)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 遺伝子改変動物 / 炎症性骨破壊

Outline of Final Research Achievements

We may find the technique that is useful in prevention or treatment of disease with bone destruction by investigating roles of Rho family GTPases in bone resorption. Therefore, we generated osteoclast-specific inactivated Rac1 and Cdc42 mice to investigate the physiological functions of Rac1 and Cdc42 in bone resorption.
As a result, our findings suggest that Rac1 and Cdc42 play important roles for bone resorption caused by osteoclast formation in vivo.

Research Products

• [Journal Article] Cdc42 is critical for cartilage development during endochondral ossification. (2015)
  • Author(s): Suzuki W, Yamada A, Aizawa R, Suzuki D, Kassai H, Harada T, Nakayama M, Nagahama R, Maki K, Takeda S, Yamamoto M, Aiba A, Baba K, Kamijo R
  • Journal Title: Endocrinology Volume: 156 Issue: 1 Pages: 314-322
  • DOI: 10.1210/en.2014-1032
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cdc42 plays important roles in bone formation during limb development. (2013)
  • Author(s): Aizawa R, Yamada A, Suzuki D, Tsukasaki M, Yamamoto M, Aiba A, Kamijo R
  • Journal Title: Jpn. J. Tissue Cult. Dent. Res. Volume: 22 Pages: 35-41
  • Peer Reviewed
• [Journal Article] BMP-2 induced expression of Alx3 that is a positive regulator of osteoblast differentiation. (2013)
  • Author(s): Matsumoto, T., Yamada, A., Suzuki, D., Aizawa, R., Tsukasaki, M., Yamamoto, M., Baba, K., Kamijo, R.
  • Journal Title: Plos One Volume: 8 Issue: 6 Pages: e68774-e68774
  • DOI: 10.1371/journal.pone.0068774
  • Peer Reviewed
• [Presentation] Cdc42は口蓋形成過程において必須の遺伝子である． (2014)
  • Author(s): 中山睦子，山田 篤，鈴木 大，相澤 怜，鈴木 航，馬場一美，山本松男，上條竜太郎，槇宏太郎
  • Organizer: 第61回昭和大学学士会総会
  • Place of Presentation: 東京
  • Year and Date: 2014-12-06
• [Presentation] Cdc42 is essential for cartilage development during endochondral ossification. (2014)
  • Author(s): Suzuki W, Yamada A, Aizawa R, Suzuki D, Takeda S, Yamamoto M, Baba K, Kamijo R
  • Organizer: ANZBMS 24th Annual Scientific Meeting
  • Place of Presentation: Queenstown, New Zealand
  • Year and Date: 2014-09-09
• [Presentation] Cdc42は軟骨形成とそれに続く軟骨内骨化に必須である． (2014)
  • Author(s): 鈴木 航，山田 篤，相澤 怜，鈴木 大，竹田 秀，山本松男，馬場一美，上條竜太郎
  • Organizer: 第32回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2014-07-24
• [Presentation] Cdc42は軟骨形成に必須の遺伝子である． (2013)
  • Author(s): 鈴木 航，山田 篤，相澤 怜，鈴木 大，中山睦子，山本松男，槇宏太郎，馬場一美，上條竜太郎
  • Organizer: 第55回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岡山