The differentiation ability in the undifferentiated stem cell from the human dental pulp cells of deciduous teeth

Research Project

Project/Area Number	25893273
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Orthodontics/Pediatric dentistry
Research Institution	Osaka Dental University
Principal Investigator	KAWAI Saki 大阪歯科大学, 歯学部, 助教 (70707067)
Project Period (FY)	2013-08-30 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000) Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	歯髄 / 乳歯 / 未分化
Outline of Final Research Achievements	In the past study, it was reported that 6-bromoindirubin-3’-oxime（BIO）maintains undifferentiation ability in mouse and human ES cell. I examined the cell proliferation and undifferentiation ability in human dental pulp cells of deciduous teeth (hDPCs) was cultured with cell culture medium and BIO. We used MTS assay, Real time RT-PCR and Frow cytometory. We sorted hDPCs stimulated with BIO by Flow cytometry, but there was not the difference between hDPCs stimulated with BIO and hDPCs. Therefore I should examine the different culture condition. I investigate the undifferentiation ability of hDPCs in Hypoxia culture.

Report

(3 results)