| Project/Area Number |
25893275
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Kwansei Gakuin University |
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Principal Investigator |
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| Project Period (FY) |
2013-08-30 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 脱ユビキチン化 / CFTR / タンパク質品質管理 / ユビキチン |
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| Outline of Final Research Achievements |
Here, we sought to identify the deubiquitination enzyme (DUB) involved in the peripheral protein quality control mechanism that eliminates aberrant plasma membrane proteins such as ΔF508 CFTR. Analysis of a panel of DUB inhibitors discovered that inhibition of proteasome-associated DUB increased the cell surface expression and stability of ΔF508 CFTR. Moreover, endocytosis of ΔF508 CFTR was dramatically inhibited by inhibitors of proteasome-associated DUB. These results provide a novel regulatory mechanism that the proteasome-associated deubiquitination stimulates the elimination of aberrant plasma membrane proteins from the cell surface.
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