Identification of non-coding RNA associated with the proliferative and chondrogenic abilities of human bone marrow mesenchymal stem cells

• Project/Area Number: 25893277
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Mukogawa Women's University
• Principal Investigator: MERA Hisashi 武庫川女子大学, スポーツ健康科学部, 研究員 (70650381)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 関節軟骨修復・再生 / 骨髄細胞 / 軟骨分化度マーカー / miR708-5p / 関節軟骨修復 / 軟骨分化能 / non-coding RNA

Outline of Final Research Achievements

In this study, we analyzed the bone marrow mesenchymal stem cells (BMSCs) isolated from 30 donors. We could repeat the quantitative outcomes that were previously reported for the effects of bFGF on the chondrogenic ability of the BMSCs during the expansion phase. Moreover, we could obtain independent quantitative data for each donor. This finding indicates that the sample size used here is sufficient for subsequent analysis to validate the results.
The RNA profile of each donor, generated before BMSC differentiation, was analyzed comprehensively using an RNA microarray system (EXIQON Inc. miRCURY LNA miRNA array). We selected some candidate micro RNAs as markers of chondrogenic ability of BMSCs. These markers were verified by quantitative PCR, and miR708-5p was found to suppress chondrogenic differentiation. However, the variation in miR708-5p expression between donors was large, suggesting that other factors could also be involved in regulating the chondrogenic ability of BMSCs.

Research Products

• [Journal Article] Potential for Tumorigenesis and Repair Osteochondral Defects by iPS cell Transplantation in Rat. (2014)
  • Author(s): Mera H., Takigami J., Tamamura Y., Itokazu M., Yamazoe M., Hashimoto Y., Endo N., Wakitani S.
  • Journal Title: American Journal of Tissue Engineering and Stem cell. Volume: 1(1) Pages: 39-50
  • Peer Reviewed / Open Access
• [Journal Article] Cartilage Repair With Autologous Bone Marrow Mesenchymal Stem Cell Transplantation: Review of Preclinical and Clinical Studies. (2014)
  • Author(s): Yamasaki S., Mera H., Itokazu M., Hashimoto Y., Wakitani S.
  • Journal Title: Cartilage Volume: 5(4) Issue: 4 Pages: 196-202
  • DOI: 10.1177/1947603514534681
  • Peer Reviewed
• [Presentation] 骨髄間葉系細胞を用いた関節軟骨再生治療の研究開発 (2015)
  • Author(s): 目良恒、石橋宰、高木睦、脇谷滋之
  • Organizer: 第7回 日本関節鏡・膝・スポーツ整形外科学会（JOSKAS）
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2015-06-18 – 2015-06-20
  • Invited
• [Presentation] The population of human bone marrow mesenchymal stem cells supplemented with basic fibroblast growth factor for chondrogenic differentiation. (2015)
  • Author(s): Hisashi Mera, Yoshihiro Tamamura, Maki Itokazu, Miki Asada, Shigeyuki Wakitani
  • Organizer: Osteoarthritis Research Society International
  • Place of Presentation: Washington State Convention Center, Seattle, WA, (USA)
  • Year and Date: 2015-04-30 – 2015-05-03
• [Presentation] 変形性関節症と再生医療 (2014)
  • Author(s): 目良恒、脇谷滋之
  • Organizer: 第58回 日本リウマチ学会総会・学術集会
  • Place of Presentation: グランドプリンスホテル新高輪（東京都・港区）
  • Year and Date: 2014-04-24 – 2014-04-26
  • Invited
• [Presentation] ヒト骨髄細胞由来scaffold-free再生軟骨様細胞シ-ト作製の工夫 (2014)
  • Author(s): 糸数万紀、目良恒、佐藤康史、中村博亮、脇谷滋之
  • Organizer: 第27回 日本軟骨代謝学会
  • Place of Presentation: 京都府医師会館
• [Presentation] ヒト骨髄細胞由来scaffold-free再生軟骨様細胞シ-ト作製の工夫 (2014)
  • Author(s): 目良恒、糸数万紀、高木睦、脇谷滋之
  • Organizer: 第217回 新潟整形外科研究会
  • Place of Presentation: 新潟大学医学部有壬記念館
• [Presentation] 変形性関節症と再生医療 (2014)
  • Author(s): 目良恒、脇谷滋之
  • Organizer: 第58回 日本リウマチ学会
  • Place of Presentation: グランドプリンスホテル新高輪
  • Invited
• [Presentation] THE ENHANCEMENT OF HUMAN BONE MARROW DERIVED MESENCHYMAL STEM CELLS (HBMSCS) FOR SCAFFOLD-FREE CARTILAGE-LIKE CELL-SHEETS (2014)
  • Author(s): Maki Itokazu, Hisashi Mera, Yasushi Sato, Hiroaki Nakamura, Mutsumi Takagi, Shigeyuki Wakitani
  • Organizer: 2014 World congress on Osteoarturitis (OARSI)
  • Place of Presentation: The center of New Industries and Technologies, Paris, France