Promotion of in vivo autologous tissue formation by light emitting diode: Application to development of Stent Biovalve

• Project/Area Number: 25893303
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Cardiovascular surgery
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: FUNAYAMA Marina 独立行政法人国立循環器病研究センター, 研究開発基盤センター, 研究員 (30713599)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 心臓代替弁 / 肺動脈弁 / 心血管疾患 / 医療 / 心臓血管外科 / 光刺激 / 組織工学

Outline of Final Research Achievements

Surgical valve replacement is an efficacious treatment for patients with cardiovascular disease, however the artificial heart valves have limitations regarding their durability. The in-body tissue architecture (IBTA) technology is a novel technique based on the tissue encapsulation phenomenon. Using this technology, autologous self-expanding valved stent, named Stent Biovalve, could be made simple and safe. However, the limited leaflet formation through the tissue migration was a serious problem. To solve this limitation, we have firstly established an effect on IBTA of the light radiation. Secondly, we evaluated directly and non-invasively the formation process of the leaflet tissues inside the mold. Moreover, we designed novel molds to activate leaflet formation. Finally, Stent Biovalve showed favorable hemodynamic results in pulmonary valve in beagles. Taken together, the novel method may improve a development of the functional Stent Biovalve.

Research Products

• [Journal Article] Development of self-expanding valved stents with autologous tubular leaflet tissues for transcatheter valve implantation. (2015)
  • Author(s): Funayama M, Sumikura H, Takewa Y, Tatsumi E, Nakayama Y.
  • Journal Title: J Artif Organs Volume: 未定 Issue: 3 Pages: 228-235
  • DOI: 10.1007/s10047-015-0820-6
  • Peer Reviewed
• [Journal Article] 弁付導管による肺動脈弁置換術の基礎的検討 (2015)
  • Author(s): 篠田麻子、水越崇博、船山麻理菜、水野壮司、水野祐、上地正実
  • Journal Title: 日獣会誌 Volume: 68 Pages: 49-54
  • NAID: 130004888069
  • Peer Reviewed
• [Journal Article] In situ observation and enhancement of leaflet tissue formation in bioprosthetic "biovalve". (2015)
  • Author(s): Funayama M, Takewa Y, Oie T, Matsui Y, Tatsumi E, Nakayama Y.
  • Journal Title: J Artif Organs. Volume: 18(1) Issue: 1 Pages: 40-7
  • DOI: 10.1007/s10047-014-0793-x
  • Peer Reviewed
• [Journal Article] Successful implantation of autologous valved conduits with self-expanding stent (stent-biovalve) within the pulmonary artery in beagle dogs. (2015)
  • Author(s): Funayama M, Matsui Y, Tajikawa T, Sasagawa T, Saito Y, Sagishima S, Mizuno T, Mizuno M, Harada K, Uchida S, Shinoda A, Iwai R, Nakayama Y, Uechi M.
  • Journal Title: J Vet Cardiol. Volume: 17(1) Issue: 1 Pages: 54-61
  • DOI: 10.1016/j.jvc.2014.12.003
  • Peer Reviewed
• [Journal Article] 慢性腎臓病の犬における血中オルビフロキサシン濃度の評価 (2014)
  • Author(s): 船山麻理菜、上地正実
  • Journal Title: 日獣会誌 Volume: 67 Pages: 603-607
  • NAID: 130004692167
  • Peer Reviewed
• [Presentation] In vivo tissue-engineered, autologous, valved conduit "Biovalve" with robust wall tissues (2015)
  • Author(s): M. Funayama, M. Furukoshi, T. Moriwaki, Y. Nakayama
  • Organizer: European Society Cardiology Congress 2015
  • Place of Presentation: London, United Kingdom
  • Year and Date: 2015-08-29 – 2015-09-02
• [Presentation] 小型から大型までバイオバルブ作製の確実化 (2015)
  • Author(s): 船山麻理菜、古越真耶、森脇健司、中山泰秀
  • Organizer: 第14回日本再生医療学会
  • Place of Presentation: 横浜
  • Year and Date: 2015-03-19 – 2015-03-21
• [Presentation] カゴ化鋳型の開発と、丈夫な導管を有するバイオバルブの作製と、安心した移植 (2015)
  • Author(s): 船山麻理菜、古越真耶、森脇健司、中山泰秀
  • Organizer: 第14回日本再生医療学会
  • Place of Presentation: 横浜
  • Year and Date: 2015-03-19 – 2015-03-21
• [Presentation] 僧帽弁閉鎖不全症の犬72例における歯周病原細菌の検出と心不全重症度の関連 (2015)
  • Author(s): 船山麻理菜、山代久美子、倉松俊亘、水野祐、水野壮司、原田佳代子、高橋新音、渡辺清子、上地正実
  • Organizer: 第11回日本獣医内科学アカデミー学術大会
  • Place of Presentation: 横浜
  • Year and Date: 2015-02-20 – 2015-02-22
• [Presentation] 脳動脈瘤塞栓治療用多孔薄膜カバードステントの開発：外弯側動脈瘤モデルにおける塞栓可能性の検討 (2015)
  • Author(s): 森脇健司、中山泰秀、日高涼、佐藤徹、濱野栄佳、石井大造、林正孝、船山麻理菜、古越真耶、田地川勉
  • Organizer: 第27回バイオエンジニアリング講演会
  • Place of Presentation: 新潟
  • Year and Date: 2015-01-09 – 2015-01-10
• [Presentation] Tissu-engineered pulmonary valve (biovalve) replacement in dogs with congenital pulmonic stenosis. (2014)
  • Author(s): T. Mizuno, H. Takano, M. Furukoshi, M.Funayama, M. Mizuno, K. Harada, Y.Nakayama, M. Uechi
  • Organizer: The 4th Annual Congress of Asian Society of Veterinary Surgery
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2014-12-06 – 2014-12-07
• [Presentation] 国立循環器病研究センターにおける医療機器開発時の非臨床試験に対する信頼性保証動物試験体制の構築 (2014)
  • Author(s): 水野敏秀、稲垣悦子、中野敦、船山麻理菜、岩田倫明、築谷朋典、武輪能明、巽英介、北風政史
  • Organizer: 第52回日本人工臓器学会
  • Place of Presentation: 札幌
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] ２種類のチューブ型ステント付バイオバルブの肺動脈移植による生体内機能評価 (2014)
  • Author(s): 船山麻理菜、水野壮司、山南将志、神田圭一、上地正実、中山泰秀
  • Organizer: 第52回日本人工臓器学会
  • Place of Presentation: 札幌
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] いよいよ来年度に迫った脳動脈瘤治療用カバードステントの医師主導治験 (2014)
  • Author(s): 中山泰秀、佐藤徹、船山麻理菜、森脇健司、田地川勉、濱野栄佳、石井大造、林正孝、川尻英長、水野壮司、古越真耶、西正吾、高橋淳
  • Organizer: 第52回日本人工臓器学会
  • Place of Presentation: 札幌
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] Successful one-device-treatment of intercranial giant and wide neck aneurysms (2014)
  • Author(s): Y.Nakayama, T.Satow, T.Mizuno, T.Moriwaki, E. Hamano, D.Ishi, M.Hayashi, H.Kawajiri, M.Funayama
  • Organizer: 41st Annual ESAO Congress
  • Place of Presentation: Roma, Italy
  • Year and Date: 2014-09-17 – 2014-09-20
• [Presentation] 脳動脈瘤治療用多孔性薄膜カバードステントの開発 (2014)
  • Author(s): 佐藤徹、中山泰秀、濱野栄佳、石井大造、林正孝、船山麻理菜、森脇健司、坂井信幸、高橋淳
  • Organizer: 第20回日本血管内治療学会
  • Place of Presentation: 和歌山
  • Year and Date: 2014-06-20 – 2014-06-21
• [Presentation] 自己組織からなる弁葉反転型ステント付バイオバルブの肺動脈移植 (2014)
  • Author(s): 船山麻理菜、上地正実、水野壮司、逢坂真吾、田地川勉、中山泰秀
  • Organizer: 第100回日本獣医循環器学会
  • Place of Presentation: 大宮
  • Year and Date: 2014-06-14 – 2014-06-15
• [Presentation] 生体内でつくる心臓代替弁の三尖弁移植 (2014)
  • Author(s): 栩木叶子、船山麻理菜、水野壮司、水野祐、原田佳代子、水越崇博、上地正実
  • Organizer: 第100回日本獣医循環器学会
  • Place of Presentation: 大宮
  • Year and Date: 2014-06-14 – 2014-06-15
• [Presentation] Establishment of Experimental Animal Models Suitable for Feasibility Testing in the Development of Covered Stent for Intracranial Aneurysms (2014)
  • Author(s): T.Satow, Y.Nakayama, E Hamano, T Mizuno, M.Funayama, D Ishii, M Hayashi, T Moriwaki, H Kawajiri, S Nishi
  • Organizer: ASNR 52nd Annual Meeting & The Foundation of the ASNR Symposium 2014
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2014-05-17 – 2014-05-22
• [Presentation] Successful 1-device treatment of intracranial giant and/or wide neck aneursyms: 3 essential in vivo experimental designs for pre-clinical evaluation (2014)
  • Author(s): Y.Nakayama, T.Satow, E.Hamano, T.Mizuno, M.Funayama, D.Ishii, M.Hayashi, T.Moriwaki, H.Kawajiri, K.Ihara
  • Organizer: 63th International Congress of the European Society for Cardiovascular and Endovascular Surgery
  • Place of Presentation: Nice, France
  • Year and Date: 2014-04-24 – 2014-04-27