Organogenesis of nasopharynx- and tear duct-associated lymphoid tissues

• Project/Area Number: 25893306
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: 独立行政法人医薬基盤研究所
• Principal Investigator: NAGATAKE Takahiro 独立行政法人医薬基盤研究所, 創薬基盤研究部, 研究員 (80608737)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 粘膜関連リンパ組織 / リンパ組織誘導細胞 / 組織形成 / 粘膜免疫

Outline of Final Research Achievements

CD3-CD4+CD45+ LTi cells plays a central role in mucosa-associated lymphoid tissues. In this study, we characterized and compared biological function of LTi cells in Peyer's patches (PPs), nasopharynx-associated lymphoid tissue (NALT) and tear duct-associated lymphoid tissue (TALT). It is found that PP-LTi cells expressed high amount of lymphotoxin which is essential for the genesis of PPs. In contrast, lymphotoxin was not expressed by NALT- and TALT-LTi cells. We also found that Cbfb2, a transcriptional regulator, was expressed in PPs-, NALT- and TALT-LTi cells. Cbfb2-deficient mice showed impairment of PPs-, NALT- and TALT-development. These results indicate that Cbfb2 is essential molecule for mucosa-associated lymphoid tissues.

Research Products

• [Journal Article] Central role of core binding factor β2 in mucosa-associated lymphoid tissue organogenesis in mouse (2015)
  • Author(s): Takahiro Nagatake, Satoshi Fukuyama, Shintaro Sato, Hideaki Okura, Masashi Tachibana, Ichiro Taniuchi, Kosei Ito, Michiko Shimojou, Naomi Matsumoto, Hidehiko Suzuki, Jun Kunisawa, and Hiroshi Kiyono
  • Journal Title: PLoS ONE Volume: 未定
  • NAID: 120006987038
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Enteroendocrine cells are specifically marked by cell surface expression of claudin-4 in mouse small intestine (2014)
  • Author(s): Nagatake T, Fujita H, Minato N, Hamazaki Y.
  • Journal Title: PLoS One Volume: 9 Issue: 3 Pages: e90638-e90638
  • DOI: 10.1371/journal.pone.0090638
  • Peer Reviewed / Open Access
• [Journal Article] Unique functions of mucosa-associated lymphoid tissues as targets of mucosal vaccines (2013)
  • Author(s): Takahiro Nagatake and Jun Kunisawa
  • Journal Title: Current topics in pharmacology Volume: 17 Pages: 13-23
• [Presentation] Immunological and organogenesis diversity of mucosa-associated lymphoid tissues for the development of mucosal vaccine (2015)
  • Author(s): Takahiro Nagatake, Naomi Matsumoto, Michiko Shimojou, Hidehiko Suzuki, Satoshi Fukuyama, Shintaro Sato, Kentaro Ogami, Yusuke Tsujimura, Mitsuo Kawano, Tetsuya Nosaka, Hiroshi Kiyono, Yasuhiro Yasutomi, and Jun Kunisawa
  • Organizer: 第8回次世代アジュバント研究会
  • Place of Presentation: 大阪
  • Year and Date: 2015-01-20
• [Presentation] Eye immune responses initiated by uniquely developing tear duct-associated lymphoid tissue (2013)
  • Author(s): Takahiro Nagatake, Shintaro Sato, Jun Kunisawa and Hiroshi Kiyono
  • Organizer: 日本免疫学会
  • Place of Presentation: 幕張メッセ（千葉）
• [Book] 医学のあゆみ-生命を支える脂質 最新の研究と臨床-（企画: 新井洋由） (2014)
  • Author(s): 長竹貴広、國澤 純
  • Total Pages: 284
  • Publisher: 医歯薬出版株式会社