A Single-Cell-RNA Sequencing Based Strategy to Identify Host Cell Types and Cell Targets for Host-Microbiota Interaction in Calf Diarrhea

• Project/Area Number: 25K00264
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 42010:Animal production science-related
• Research Institution: Tohoku University
• Principal Investigator: イスラム ジャヒドゥル 東北大学, 農学研究科, 特任助教 (20805569)
• Project Period (FY): 2025-04-01 – 2028-03-31
• Project Status: Granted (Fiscal Year 2025)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2027: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2026: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2025: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: scRNA-seq / Cow / Machine learning

Outline of Research at the Start

Calf diarrhea remains a significant cause of productivity and economic loss. Newborn calves have low gastrointestinal barrier function and immunity before weaning, making them highly susceptible to infection by various pathogens. During diarrhea, the epithelial cells lining the small and large intestines can be severely damaged, trigger an inflammatory response, reduces the surface area available for nutrient and water absorption. Therefore, it is crucial to identify specific host cell types and targets alongside microbiome analysis to design optimal treatments for calf diarrhea,