A fission yeast chemical-genomics platform for systematic functional annotation of novel bioactive compounds

Research Project

Project/Area Number	25K01916
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 37030:Chemical biology-related
Research Institution	Institute of Physical and Chemical Research
Principal Investigator	BOONE CHARLES 国立研究開発法人理化学研究所, 環境資源科学研究センター, チームディレクター (70601342)
Co-Investigator(Kenkyū-buntansha)	Pham LienThiKim 国立研究開発法人理化学研究所, 環境資源科学研究センター, 研究員 (50865300) 八代田陽子国立研究開発法人理化学研究所, 環境資源科学研究センター, 副チームディレクター (60360658)
Project Period (FY)	2025-04-01 – 2028-03-31
Project Status	Granted (Fiscal Year 2025)
Budget Amount *help	¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000) Fiscal Year 2027: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2026: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000) Fiscal Year 2025: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywords	Chemical-genomics / S. pombe / Target identification
Outline of Research at the Start	As a systematic chemical-genetic interaction profiling platform for linking compounds with their cellular target pathways, we develop two new S. pombe barcoded mutant collections: a set of haploid gene deletion mutants covering non-essential genes and a heterozygous diploid deletion mutant collection covering essential genes in a drug-hypersensitive genetic background. We plan to utilize this new platform to screen functionally uncharacterized bioactive compounds and focus on detailed mechanistic characterization of compounds whose predicted targets are conserved from yeast to human cells.