| Project/Area Number |
25K18414
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 43020:Structural biochemistry-related
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
謝 銘輝 奈良先端科学技術大学院大学, 先端科学技術研究科, 助教 (60834455)
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| Project Period (FY) |
2025-04-01 – 2027-03-31
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| Project Status |
Granted (Fiscal Year 2025)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2026: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2025: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | PHA synthase / Biodegradable plastic / Dimer / Enzyme intermediate / catalytic mechanisms |
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| Outline of Research at the Start |
PHA synthase (PhaC) is an enzyme that produces biodegradable plastics (PHAs), with a preference for short-chain substrates over medium-chain ones. However, the structural basis of this specificity is unclear. This project aims to reveal intermediate structures of PhaC with its substrates using X-ray crystallography and cryo-EM. By analyzing homologs from different organisms and performing protein engineering, we aim to uncover key structural features that control substrate preference. These insights will support future enzyme engineering efforts.
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