Development of the MRSA All-in-One Gene Detection Array for Enhanced Resistance Gene Profiling and Bacterial Genotyping

• Project/Area Number: 25K18999
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 52010:General internal medicine-related
• Research Institution: Jichi Medical University
• Principal Investigator: タン シンイー 自治医科大学, 医学部, 助教 (70828475)
• Project Period (FY): 2025-04-01 – 2027-03-31
• Project Status: Granted (Fiscal Year 2025)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2026: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2025: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: Gene Detection Array / Phage / CRISPR

Outline of Research at the Start

This study proposed to develop a MRSA All-in-One Gene Detection Array using a refined CRISPR-Cas13a system. The proposed array is anticipated to profile bacterial genetics within a shorter turnaround time, easier to perform, and affordable, without compromising detection sensitivity/specificity.