金属ナノ粒子による細胞内分子イメージング

• Project/Area Number: 26000011
• Research Category: Grant-in-Aid for Specially Promoted Research
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering; Engineering
• Research Institution: 有限会社セレンディップ研究所 (2017-2018); Osaka University (2014-2016)
• Principal Investigator: 河田 聡 有限会社セレンディップ研究所, 研究開発部, 主任研究員 (30144439)
• Co-Investigator(Kenkyū-buntansha): 田端 和仁 東京大学, 工学研究科, 講師 (50403001) ; 藤田 克昌 大阪大学, 工学研究科, 准教授 (80362664) ; 野地 博行 東京大学, 工学系研究科, 教授 (00343111)
• Project Period (FY): 2014 – 2018
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥522,080,000 (Direct Cost: ¥401,600,000、Indirect Cost: ¥120,480,000); Fiscal Year 2018: ¥52,260,000 (Direct Cost: ¥40,200,000、Indirect Cost: ¥12,060,000); Fiscal Year 2017: ¥69,810,000 (Direct Cost: ¥53,700,000、Indirect Cost: ¥16,110,000); Fiscal Year 2016: ¥117,390,000 (Direct Cost: ¥90,300,000、Indirect Cost: ¥27,090,000); Fiscal Year 2015: ¥137,800,000 (Direct Cost: ¥106,000,000、Indirect Cost: ¥31,800,000); Fiscal Year 2014: ¥144,820,000 (Direct Cost: ¥111,400,000、Indirect Cost: ¥33,420,000)
• Keywords: ナノフォトニクス / プラズモニクス / ナノイメージング / バイオイメージング / ラマン散乱分光 / 表面増強ラマン散乱 / 金属ナノ微粒子 / 超解像顕微鏡 / 細胞内分子イメージング / ラマン分光 / 超解像イメージング / 細胞内センシング / スペクトル解析 / 金属ナノ粒子 / 機能性分子

Outline of Annual Research Achievements

本研究計画では、細胞内分子イメージングのための「3次元・ナノ・分析・光学」の顕微鏡開発を目的とし、昨年度には下記の4つの項目を達成した。
1、細胞内3次元追跡の高速化、マルチプローブ計測の実現
細胞内の金属ナノ微粒子を追跡しながら周囲の分子をセンシングする技術の向上に取り組んだ。様々な形状・種類のナノ微粒子の合成と、励起波長の最適化により生きた細胞内部で1msの時間分解能と30nmの位置精度でのナノ微粒子の3次元追跡及び表面増強ラマン散乱(SERS)分光計測を達成した。また、複数の金属ナノ微粒子を細胞内で同時に追跡・SERS分光計測が可能な顕微鏡の開発にも成功した。
2、機能的・高感度な金属ナノ微粒子プローブによる細胞内イメージング
開発した高感度で安定なSERS検出を可能とするコア-サテライト状金属ナノ微粒子をp-MBAにより修飾し、pHセンシング機能を付加した。修飾した金属ナノ微粒子を細胞内に導入し、アポトーシス時の局所領域でのpHを経時的に測定した。アポトーシスの進行による細胞形態の変化に伴いpHの変化が検出された。細胞内環境変化を金属ナノ微粒子を用いて検出することに成功した。
3、薬剤取り込み過程のリアルタイム計測
細胞の3次元イメージングが可能なラマン散乱顕微鏡を開発し、薬剤を付加した細胞の経時観察を行った。細胞へは金属ナノ微粒子を事前に取り込ませ、薬剤が細胞内に到達した場所と時間を、薬剤に付加したアルキンのSERSにより検出した。細胞への薬剤の付加後、数十分での薬剤取込・取込量と薬剤濃度との相関・この薬剤の取り込みにはエンドサイトーシスが関与しないことを確認した。
4、深紫外領域での応用を目指した金属ナノ微粒子の合成と評価
プラズモン共鳴波長が深紫外域となるインジウムナノ微粒子の合成をした。インジウムナノ微粒子を塗布した石英基盤上にアデニンを付加し、生体分子の深紫外SERSを確認した。インジウムナノ微粒子による局所増強効果を利用した生体内での深紫外ラマン分光計測実現の可能性が示唆された。

Assessment Rating

Verification Result (Rating)

A

Research Products

• [Int'l Joint Research] Stockholm University/Nordic Institute for Theoretical Physics(スウェーデン)
• [Int'l Joint Research] University of Otago(ニュージーランド)
• [Int'l Joint Research] (台湾)
• [Journal Article] High-Throughput Cell Imaging and Classification by Narrowband and Low-Spectral-Resolution Raman Microscopy (2019)
  • Author(s): Kumamoto Yasuaki、Mochizuki Kentaro、Hashimoto Kosuke、Harada Yoshinori、Tanaka Hideo、Fujita Katsumasa
  • Journal Title: The Journal of Physical Chemistry B Volume: 123 Issue: 12 Pages: 2654-2661
  • DOI: 10.1021/acs.jpcb.8b11295
  • Peer Reviewed / Open Access
• [Journal Article] Correlative force and tip-enhanced Raman microscopy (2019)
  • Author(s): Saitoh Kohta、Taguchi Atsushi、Kawata Satoshi
  • Journal Title: APL Photonics Volume: 4 Issue: 2 Pages: 021301-021301
  • DOI: 10.1063/1.5064546
  • NAID: 130007702850
  • Peer Reviewed / Open Access
• [Journal Article] Using redox-sensitive mitochondrial cytochrome Raman bands for label-free detection of mitochondrial dysfunction. (2019)
  • Author(s): Morimoto T, Chiu LD, Kanda H, Kawagoe H, Ozawa T, Nakamura M, Nishida K, Fujita K, Fujikado T
  • Journal Title: Analyst Volume: 144 Issue: 8 Pages: 25312540-25312540
  • DOI: 10.1039/c8an02213e
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Quantitative evaluation of SERS nanoparticles for intracellular pH sensing at a single particle level (2019)
  • Author(s): Zhiqiang Zhang, Kazuki Bando, Kentaro Mochizuki, Atsushi Taguchi, Katsumasa Fujita, and Satoshi Kawata
  • Journal Title: Anal. Chem Volume: 91 Issue: 5 Pages: 3254-3262
  • DOI: 10.1021/acs.analchem.8b03276
  • Peer Reviewed / Open Access
• [Journal Article] Regeneration of Escherichia coli giant protoplasts to their original form. (2019)
  • Author(s): *Tabata KV, Sogo T, Moriizumi Y, Noji H.
  • Journal Title: Life Volume: 9 Issue: 1 Pages: 1-10
  • DOI: 10.3390/life9010024
  • Peer Reviewed / Open Access
• [Journal Article] Osmolyte-Enhanced Protein Synthesis Activity of a Reconstituted Translation System (2019)
  • Author(s): Moriizumi Yoshiki、Tabata Kazuhito V.、Miyoshi Daisuke、Noji Hiroyuki
  • Journal Title: ACS Synthetic Biology Volume: 8 Issue: 3 Pages: 557-567
  • DOI: 10.1021/acssynbio.8b00513
  • Peer Reviewed / Open Access
• [Journal Article] Antibody-free digital influenza virus counting based on neuraminidase activity (2019)
  • Author(s): Tabata Kazuhito V.、Minagawa Yoshihiro、Kawaguchi Yuko、Ono Mana、Moriizumi Yoshiki、Yamayoshi Seiya、Fujioka Yoichiro、Ohba Yusuke、Kawaoka Yoshihiro、Noji Hiroyuki
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 1067-1067
  • DOI: 10.1038/s41598-018-37994-6
  • Peer Reviewed / Open Access
• [Journal Article] Deep‐Ultraviolet Biomolecular Imaging and Analysis (2018)
  • Author(s): Kumamoto Yasuaki、Taguchi Atsushi、Kawata Satoshi
  • Journal Title: Advanced Optical Materials Volume: 7 Issue: 5 Pages: 1801099-1801099
  • DOI: 10.1002/adom.201801099
  • Peer Reviewed / Open Access
• [Journal Article] Saturated excitation microscopy using differential excitation for efficient detection of nonlinear fluorescence signals (2018)
  • Author(s): Yasunori Nawa, Yasuo Yonemaru, Atsushi Kasai, Ryosuke Oketani, Hitoshi Hashimoto, Nicholas I. Smith, and Katsumasa Fujita
  • Journal Title: APL Photonics Volume: 3 Issue: 8 Pages: 808051-12
  • DOI: 10.1063/1.5039567
  • Peer Reviewed / Open Access
• [Journal Article] Hybrid cell reactor system from Escherichia coli protoplast cells and arrayed lipid bilayer chamber device. (2018)
  • Author(s): Moriizumi Y, *Tabata KV, Watanabe R, Doura T, Kamiya M, Urano Y, Noji H.
  • Journal Title: Sci Rep. Volume: 8 Issue: 1 Pages: 1-13
  • DOI: 10.1038/s41598-018-30231-0
  • Peer Reviewed / Open Access
• [Journal Article] High-throughput single-molecule bioassay using micro-reactor arrays with a concentration gradient of target molecules (2018)
  • Author(s): Watanabe R, Komatsu T, Sakamoto S, Urano Y, Noji H
  • Journal Title: Lab Chip Volume: 18 Pages: 2849-2853
  • Peer Reviewed / Open Access
• [Journal Article] Digital enzyme assay using attoliter droplet array. (2018)
  • Author(s): Ono T, Ichiki T, Noji H
  • Journal Title: Analyst Volume: 143 Issue: 20 Pages: 4923-4929
  • DOI: 10.1039/c8an01152d
  • Peer Reviewed / Open Access
• [Journal Article] Off-axis rotor in Enterococcus hirae V-ATPase visualized by Zernike phase plate single-particle cryo-electron microscopy (2018)
  • Author(s): Tsunoda Jun、Song Chihong、Imai Fabiana Lica、Takagi Junichi、Ueno Hiroshi、Murata Takeshi、Iino Ryota、Murata Kazuyoshi
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 15632-15632
  • DOI: 10.1038/s41598-018-33977-9
  • Peer Reviewed / Open Access
• [Journal Article] Structure and dynamics of rotary V1 motor (2018)
  • Author(s): Ueno Hiroshi、Suzuki Kano、Murata Takeshi
  • Journal Title: Cellular and Molecular Life Sciences Volume: 75 Issue: 10 Pages: 1789-1802
  • DOI: 10.1007/s00018-018-2758-3
  • Peer Reviewed / Open Access
• [Journal Article] High-resolution imaging in two-photon excitation microscopy using in situ estimations of the point spread function. (2018)
  • Author(s): Doi, A., Oketani, R., Nawa, Y., & Fujita, K.
  • Journal Title: Biomedical Optics Express Volume: 1 Issue: 1 Pages: 202-213
  • DOI: 10.1364/boe.9.000202
  • Peer Reviewed / Open Access
• [Journal Article] Resolution enhancement in deep-tissue nanoparticle imaging based on plasmonic saturated excitation microscopy (2018)
  • Author(s): Deka, G., Nishida, K., Mochizuki, K., Ding, H., Fujita, K. and Chu, S.
  • Journal Title: APL Photonics Volume: 3 Issue: 3 Pages: 1-11
  • DOI: 10.1063/1.5021455
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Rate constants, processivity, and productive binding ratio of chitinase A revealed by single- molecule analysis (2018)
  • Author(s): Akihiko Nakamura, Tomoyuki Tasaki, Yasuko Okuni, Chihong Song, Kazuyoshi Murata, Toshiya Kozai, Mayu Hara, Hayuki Sugimoto, Kazushi Suzuki, Takeshi Watanabe, Takayuki Uchihashi, Hiroyuki Noji and Ryota Iino
  • Journal Title: Phys. Chem. Chem. Phys. Volume: 20 Issue: 5 Pages: 3010-3018
  • DOI: 10.1039/c7cp04606e
  • Peer Reviewed / Open Access
• [Journal Article] Au-Protected Ag Core/Satellite Nanoassemblies for Excellent Extra-/Intracellular Surface-Enhanced Raman Scattering Activity (2017)
  • Author(s): Zhang, Z., Bando, K., Taguchi, A., Mochizuki, K., Sato, K., Yasuda, H., Fujita, K. and Kawata, S.
  • Journal Title: ACS Appl. Mater. Interfaces Volume: 9 Issue: 50 Pages: 44027-44037
  • DOI: 10.1021/acsami.7b14976
  • Peer Reviewed / Open Access
• [Journal Article] Tip-enhanced Raman spectroscopy -from early developments to recent advances. (2017)
  • Author(s): Deckert-Gaudig, T., Taguchi, A., Kawata, S
  • Journal Title: Chem. Soc. Rev. Chem. Soc. Rev Volume: 46 Issue: 13 Pages: 4077-4110
  • DOI: 10.1039/c7cs00209b
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Protein expression guided chemical profiling of living cells by hybrid fluorescence-Raman microscopy (2017)
  • Author(s): Liang-da Chiu , Taro Ichimura , Takumasa Sekiya , Hiroaki Machiyama , Tomonobu Watanabe , Hideaki Fujita , Takeaki Ozawa, Katsumasa Fujita
  • Journal Title: Sci. Rep. Volume: 7 Issue: 1 Pages: 43569-43569
  • DOI: 10.1038/srep43569
  • Peer Reviewed / Open Access
• [Journal Article] Nano-Raman Scattering Microscopy : Resolution and Enhancement. (2017)
  • Author(s): Kawata, S., Ichimura, T., Taguchi, A., & Kumamoto, Y.
  • Journal Title: Chemical Reviews Volume: 117 Issue: 3 Pages: 4983-5001
  • DOI: 10.1007/s10043-017-0317-z
  • Peer Reviewed / Open Access
• [Journal Article] Saturated two-photon excitation fluorescence microscopy with core-ring illumination (2017)
  • Author(s): R. Oketani, A. Doi, N. I. Smith, Y. Nawa, S. Kawata, and K. Fujita
  • Journal Title: Optics Letters Volume: 42 Issue: 3 Pages: 571-574
  • DOI: 10.1364/ol.42.000571
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Direct Measurement of Single-Molecule Adenosine Triphosphatase Hydrolysis Dynamics. (2017)
  • Author(s): Li, J., He, G., Hiroshi, U., Liu, W., Noji, H., Qi, C., & Guo, X.
  • Journal Title: ACS Nano Volume: 11 Issue: 12 Pages: 12789-12795
  • DOI: 10.1021/acsnano.7b07639
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mechano-Sensitive Synthetic Ion Channels (2017)
  • Author(s): Takahiro Muraoka, Kaori Umetsu, Kazuhito V. Tabata, Tsutomu Hamada, Hiroyuki Noji, Takashi Yamashita, and Kazushi Kinbara
  • Journal Title: J. Am. Chem. Soc. Volume: 139 Issue: 49 Pages: 18016-18023
  • DOI: 10.1021/jacs.7b09515
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Catalytic robustness and torque generation of the F1-ATPase (2017)
  • Author(s): Hiroyuki Noji, Hiroshi Ueno and Duncan G. G. McMillan
  • Journal Title: Biophysical reviews Volume: 9 Issue: 2 Pages: 103-103
  • DOI: 10.1007/s12551-017-0262-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] High Throughput Analysis of Membrane Transport by using Arrayed Water-In-Oil Droplet Bilayers (2017)
  • Author(s): R. Watanabe, N. Soga, H. Noji
  • Journal Title: Biophysical Journal Volume: 112 Issue: 3 Pages: 275a-275a
  • DOI: 10.1016/j.bpj.2016.11.1492
  • Peer Reviewed / Open Access
• [Journal Article] Alkyne-tag SERS screening and identification of small-molecule-binding sites in protein (2016)
  • Author(s): Jun Ando, Miwako Asanuma, Kosuke Dodo, Hiroyuki Yamakoshi, Satoshi Kawata, Katsumasa Fujita, Mikiko Sodeoka
  • Journal Title: Journal of the American Chemical Society Volume: 138 Issue: 42 Pages: 13901-13910
  • DOI: 10.1021/jacs.6b06003
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Invited article : Plasmonic growth of patterned metamaterials with fractal geometry (2016)
  • Author(s): N. Takeyasu, N. Taguchi, N. Nishimura, B.-H. Cheng, S. Kawata
  • Journal Title: APL Photonics Volume: 1 Issue: 5
  • DOI: 10.1063/1.4952997
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Study of nonlinear plasmonic scattering in metallic nanoparticles (2016)
  • Author(s): Y.-T. Chen, P.-H. Lee, P.-T. Shen, J. Launer, R. Oketani, K.-Y. Li, Y.-T. Huang, K. Masui, S. Shoji, K. Fujita, S.-W. Chu
  • Journal Title: ACS Photonics Volume: 3 Issue: 8 Pages: 1432-1439
  • DOI: 10.1021/acsphotonics.6b00025
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ultrasmall all-optical plasmonic switch and its application to super resolution imaging (2016)
  • Author(s): H.-Y. Wu, Y.-T. Huang, P.-T. Shen, H. Lee, R. Oketani, Y. Yonemaru, M. Yamanaka, S. Shoji, S. Kawata, C.-W. Chang, K.-H. Lin, K. Fujita, and S.-W. Chu
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 24293-24293
  • DOI: 10.1038/srep24293
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mcasurement of scattering nonlinearities from a single plasmonic nanoparticle (2016)
  • Author(s): H. Lee, K.-Y, Li, Y. -T. Huang, P. -T. Shen, G. Deka, R. Oketani, Y. Yonemaru, M. Yamanaka, K. Fujita, and S. -W. Chu
  • Journal Title: Journal of Visualizod Experiments Volume: 107 Issue: 107
  • DOI: 10.3791/53338
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Deep-UV boilogical imaging by lantanide ion molecular protection (2016)
  • Author(s): Y. Kumamoto, K. Fujita, N. I. Smith, and S. Kawata
  • Journal Title: Biomedical Optics Express Volume: 7 Issue: 1 Pages: 158-170
  • DOI: 10.1364/boe.7.000158
  • Peer Reviewed / Open Access
• [Journal Article] Arrayed water-in-oil droplet bilayers for membrane transport analysis (2016)
  • Author(s): Watanabe, R., Soga, N., Hara, M., & Noji, H
  • Journal Title: Lab on a Chip Volume: NA Issue: 16 Pages: 3043-3048
  • DOI: 10.1039/c6lc00155f
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Direct real-time detection of single proteins using silicon nanowire-based electrical circuits (2016)
  • Author(s): Jie Li, Gen He, Hiroshi Ueno, Chuancheng Jia, Hiroyuki Noji, Chuanmin Qi, Xuefeng Guo
  • Journal Title: Nanoscale Volume: 8 Issue: 36 Pages: 16172-16176
  • DOI: 10.1039/c6nr04103e
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Rotation of artificial rotor axles in rotary molecular motors (2016)
  • Author(s): Baba M, Iwamoto K, Iino R, Ueno H, Hara M, Nakanishia A, Kishikawa J, Noji H, Yokoyama K
  • Journal Title: Proc Natl Acad Sci USA Volume: 113 Issue: 40 Pages: 11214-11219
  • DOI: 10.1073/pnas.1605640113
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Biophysical Characterization of a Thermoalkaliphilic Molecular Motor with a High Stepping Torque Gives Insight into Evolutionary ATP Synthase Adaptation (2016)
  • Author(s): Duncan G. G. McMillan, Rikiya Watanabe, Hiroshi Ueno, Gregory M. Cook, and Hiroyuki Noji
  • Journal Title: Journal of Biological Chemistry Volume: 291 Issue: 46 Pages: 23965-23977
  • DOI: 10.1074/jbc.m116.743633
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Single-molecule imaging analysis of binding, processive movement, and dissociation of cellobiohydrolase Trichoderma reesei Cel6A and its domains on crystalline cellulose (2016)
  • Author(s): Nakamura A, Tasaki T, Ishiwata D, Yamamoto M, Okuni Y, Visootsat A, Maximilien M, Noji H, Uchiyama T, Samejima M, Igarashi K, Iino R
  • Journal Title: J. Biol. Chem. Volume: 291 Issue: 43 Pages: 22404-22413
  • DOI: 10.1074/jbc.m116.752048
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Biological Nanomotors with a Revolution, Linear, or Rotation Motion Mechanism (2016)
  • Author(s): P. Guo, H. Noji, CM. Yengo, Z. Zhao and I. Grainge
  • Journal Title: Microbiology and Molecular Biology Reviews Volume: 80 Issue: 1 Pages: 161-186
  • DOI: 10.1128/mmbr.00056-15
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Direct observation of intermediate states during the stepping motion of kinesin-1 (2016)
  • Author(s): Isojima H, Iino R, Niitani Y, Noji H, Tomishige M
  • Journal Title: Nat Chem Biol Volume: 12 Issue: 4 Pages: 290-297
  • DOI: 10.1038/nchembio.2028
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A microfluidic channel method for rapid drug-susceptibility testing of Pseudomonas aeruginosa (2016)
  • Author(s): Matsumoto Y, Sakakihara S, Grushnikov A, Kikuchi K, Noji H, Yamaguchi A, Iino R, Yagi Y, Nishino K
  • Journal Title: PLOS ONE Volume: 11 Issue: 2 Pages: 1-17
  • DOI: 10.1371/journal.pone.0148797
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Direct Observation of the Allosteric Conformational Change of Kinesin-lusing Gold Nanorod and its Implication for Head-Head Coordination (2016)
  • Author(s): Y. Niitani, S. Enoki, H. Noji, R. Iino and M. Tomishige
  • Journal Title: Biophysical Society Volume: 110 Issue: 3 Pages: 193a-193a
  • DOI: 10.1016/j.bpj.2015.11.1074
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Novel nano-device to measure voltage-driven membrane transporter activity (2016)
  • Author(s): Watanabe, R., Soga, N., & Noji, H.
  • Journal Title: IEEE Transactions on Nanotechnology Volume: 15 Issue: 1 Pages: 70-73
  • DOI: 10.1109/tnano.2015.2498167
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Visible-wavelength two-photon excitation microscopy for fluorescent protein imaging. (2015)
  • Author(s): M. Yamanaka, K. Saito, N.I. Smith, Y. Arai, K. Uegaki, Y. Yonemaru, K. Mochizuki, S. Kawata, T. Nagai, K. Fujita
  • Journal Title: Journal of Biomedical Optics Volume: 20 Issue: 10 Pages: 101202-101202
  • DOI: 10.1117/1.jbo.20.10.101202
  • Peer Reviewed / Open Access
• [Journal Article] A single-molecule digital enzyme assay using alkaline phosphatase with a cumarin-based fluorogenic substrate (2015)
  • Author(s): Obayashi Y, Iino R, Noji H
  • Journal Title: Analyst Volume: 140 Issue: 15 Pages: 5065-6073
  • DOI: 10.1039/c5an00714c
  • Peer Reviewed / Open Access
• [Journal Article] Giant Acceleration of diffusion observed in a single-molecule experiment on F1-ATPase (2015)
  • Author(s): Hayashi R, Sasaki K, Nakamura S, Kudo S, Inoue Y, Noji H, Hayashi K
  • Journal Title: Physical Reviow letters Volume: 114
  • Peer Reviewed / Open Access
• [Journal Article] Attolitre-sized lipid bilayer chamber array for rapid detection of single transporters (2015)
  • Author(s): Soga, N., *Watanabe, R., & Noji, H.
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 11025-11025
  • DOI: 10.1038/srep11025
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Comprehensive chemical secretory measurement of single cells trapped in a micro-droplet array with mass spectrometry (2015)
  • Author(s): Hideaki Fujita, Tsuyoshi Esaki, Tsutomu Masujima, Akitsu Hotta, Soo Hyeon Kim, Hiroyuki Noji, Tomonobu M. Watanabe
  • Journal Title: RSC Adv. Volume: 5 Issue: 22 Pages: 16968-16971
  • DOI: 10.1039/c4ra12021c
  • Peer Reviewed / Open Access
• [Journal Article] Effects of an ATP analogue, adenosine 5′-[α-thio]-triphosphate, on F1-ATPase rotary catalysis, torque generation, and inhibited intermediated formation (2015)
  • Author(s): Yukawa A, Watanabe R and Noji H
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 458 Issue: 3 Pages: 515-519
  • DOI: 10.1016/j.bbrc.2015.01.146
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Analysis of dynamic SERS spectra measured with a nanoparticle during intracellular transportation in 3D (2015)
  • Author(s): Kazuki Bando, Nicholas I Smith, Jun Ando, Katsumasa Fujita and Satoshi Kawata
  • Journal Title: Journal of Optics Volume: 17 Issue: 11 Pages: 1-13
  • DOI: 10.1088/2040-8978/17/11/114023
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] High-speed angle-resolved imaging of single gold nanorod with microsecond temporal resolution and one degree angle precision (2015)
  • Author(s): S. Enoki, R. Iino, Y. Niitani, Y. Minagawa, M. Tomishige, H. Noji
  • Journal Title: Anal. Chem. Volume: 87 Issue: 4 Pages: 2079-2086
  • DOI: 10.1021/ac502408c
  • Peer Reviewed / Open Access
• [Journal Article] Torque transmission mechanism via DELSEED loop of F1-ATPase (2015)
  • Author(s): Watanabe R, Koyasu K, You H, Tanigawara M and Noji H
  • Journal Title: Biophysical Journal Volume: 108 Issue: 5 Pages: 1144-1152
  • DOI: 10.1016/j.bpj.2015.01.017
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Plasmonics for molecular nano-Raman imaging beyond the limits. (2019)
  • Author(s): 河田聡
  • Organizer: Nano and Photonics 2019, The castle of Mauterndorf
  • Int'l Joint Research / Invited
• [Presentation] モンテカルロ顕微鏡 : 提案と実証 (2019)
  • Author(s): 河田聡
  • Organizer: 応用物理学会春季学術講演会
• [Presentation] スペックルとコヒーレンスとインスピレーション (2019)
  • Author(s): 河田聡
  • Organizer: レーザーディスプレイ研究会
  • Invited
• [Presentation] Raman microscopy for analytical imaging of living cells (2019)
  • Author(s): 藤田克昌
  • Organizer: SPIE Photonics West
  • Int'l Joint Research / Invited
• [Presentation] ラマン顕微イメージングによる小分子検出 (2019)
  • Author(s): 藤田克昌
  • Organizer: レーザー学会学術講演会 シンポジウム
  • Invited
• [Presentation] 表面増強ラマン散乱のための機能性金属ナノ粒子合成とそれを用いた細胞内分子イメージング (2019)
  • Author(s): 畔堂 一樹、張 志強、望月健太郎、藤田 克昌、河田 聡
  • Organizer: 光・量子デバイス研究会
• [Presentation] バクテリアを微小容器に再構成する (2019)
  • Author(s): 田端和仁
  • Organizer: 第13回日本ゲノム微生物学会
  • Invited
• [Presentation] Raman microscopy and plasmonics : label-free nano-imaging of biomolecules in a living cell (2018)
  • Author(s): 河田聡
  • Organizer: 5thNIC @IIT advanced practical workshop
  • Int'l Joint Research / Invited
• [Presentation] Plasmonic self-growth of plasmonic 3D nanostructures (2018)
  • Author(s): 河田聡
  • Organizer: The Photonics Institute
  • Int'l Joint Research / Invited
• [Presentation] Plasmon holography another application of plasmon resonance. (2018)
  • Author(s): 河田聡
  • Organizer: Photonics Asia
  • Int'l Joint Research / Invited
• [Presentation] Optical 3D nano-fabrication technologies : top-down and bottom-up approaches (2018)
  • Author(s): 河田聡
  • Organizer: EPFL IMT Distinguished Lecture Series
  • Int'l Joint Research / Invited
• [Presentation] なぜいまラマン散乱顕微鏡なのか : 現状と未来 (2018)
  • Author(s): 河田聡
  • Organizer: 第12回バイオ関連化学シンポジウム2018
  • Invited
• [Presentation] Plasmonic nano-imaging of intracellular dynamics and molecular distribution in a living cell (2018)
  • Author(s): 河田聡
  • Organizer: NFO-15 Troyes
  • Int'l Joint Research / Invited
• [Presentation] Functional nanoparticles explore a living cell for intracellular dynamics and molecular distribution (2018)
  • Author(s): 河田聡
  • Organizer: SPIE Optics+Photonics
  • Int'l Joint Research / Invited
• [Presentation] Plasmonics : Exotic nanophotonics beyond the limits (2018)
  • Author(s): 河田聡
  • Organizer: ISPEMI 2018
  • Int'l Joint Research / Invited
• [Presentation] Plasmonic nano-imaging of intracellular dynamics and molecular distribution in a living cell (2018)
  • Author(s): 河田聡
  • Organizer: 40th PIERS 2018
  • Int'l Joint Research / Invited
• [Presentation] Plasmonics for nano-Raman microscopy (2018)
  • Author(s): 河田聡
  • Organizer: 尾崎幸洋先生退官記念国際シンポジウム
  • Int'l Joint Research / Invited
• [Presentation] 3D and nano photonics (2018)
  • Author(s): 河田聡
  • Organizer: RCAS Seminar
  • Int'l Joint Research / Invited
• [Presentation] Alkyne-tag Raman imaging for observation of small molecules in living cells (2018)
  • Author(s): 藤田克昌
  • Organizer: 10th Asian Photochemistry Conference
  • Int'l Joint Research / Invited
• [Presentation] アルキンタグラマンイメージングによる小分子検出 (2018)
  • Author(s): 藤田克昌
  • Organizer: 第16回 医用分光学研究会
  • Invited
• [Presentation] Large-area Raman imaging by multiple slit detection (2018)
  • Author(s): 藤田克昌
  • Organizer: SCIX2018
  • Int'l Joint Research / Invited
• [Presentation] Vibrational-tag imaging : a new modality in optical microscopy (2018)
  • Author(s): 藤田克昌
  • Organizer: SCIX2018
  • Int'l Joint Research / Invited
• [Presentation] Dynamic SERS imaging of intracellular molecules (2018)
  • Author(s): 藤田克昌
  • Organizer: JSAP-OSA Joint Symposium
  • Int'l Joint Research / Invited
• [Presentation] ラマン散乱による細胞分子イメージング技術の開発 (2018)
  • Author(s): 藤田克昌
  • Organizer: 分析化学会年会
  • Invited
• [Presentation] Raman microscopy for molecular imaging of living cells (2018)
  • Author(s): 藤田克昌
  • Organizer: The 26th International Conference on Raman spectroscopy
  • Int'l Joint Research / Invited
• [Presentation] Alkyne-tag Raman imaging for finding small molecules (2018)
  • Author(s): 藤田克昌
  • Organizer: ACS Annual Meeting
  • Int'l Joint Research / Invited
• [Presentation] Surpassing the diffraction limit in Raman microscopy (2018)
  • Author(s): 藤田克昌
  • Organizer: The 20th International Conference on Transparent Optical Networks
  • Int'l Joint Research / Invited
• [Presentation] Raman microscopy for imaging intracellular molecules (2018)
  • Author(s): 藤田克昌
  • Organizer: International Conference on Advancing Molecular Spectroscopy
  • Int'l Joint Research / Invited
• [Presentation] Super-resolution confocal microscopy using optical nonlinearity (2018)
  • Author(s): 藤田克昌
  • Organizer: OSA Imaging and Applied Optics Congress
  • Int'l Joint Research / Invited
• [Presentation] ラマン分光による細胞内部の可視化 (2018)
  • Author(s): 藤田克昌
  • Organizer: 2018年度名古屋大学物理学教室憲章記念日講演会「見えない世界を「可視化」する」
  • Invited
• [Presentation] ラマン散乱を利用した新しいバイオイメージング : 細胞分析と小分子観察 (2018)
  • Author(s): 藤田克昌
  • Organizer: 第2回JKiCイメージングセミナー
  • Invited
• [Presentation] 蛍光励起の飽和を利用した超解像レーザー走査顕微鏡 (2018)
  • Author(s): 藤田克昌
  • Organizer: 第7回蛍光ミニシンポジウム
  • Invited
• [Presentation] 照明法の工夫によるラマンイメージングの高速化・高解像度化 (2018)
  • Author(s): 藤田克昌
  • Organizer: オプトロニクスセミナー
  • Invited
• [Presentation] High-Speed and High-Resolution Raman Imaging for Biological Applications (2018)
  • Author(s): 藤田克昌
  • Organizer: 日本台湾ラマン分光学に関する国際シンポジウム(Japan/Taiwan International Symposium on Raman Spectroscopy)
  • Int'l Joint Research / Invited
• [Presentation] Second harmonic generation from plasmonic nanoparticles in DUV (2018)
  • Author(s): Atsushi Taguchi
  • Organizer: SPIE Optics & Photonics, San Diego Convention Center
  • Int'l Joint Research
• [Presentation] 単一アルミニウムナノディスクからの深紫外第二高調波発生 (2018)
  • Author(s): 新田 将, 田口 敦清, 藤田 克昌
  • Organizer: 第65回応用物理学会春季学術講演会
• [Presentation] Synthesis of gold coated Sliver nano-satellite for intracellular SERS measurement (2018)
  • Author(s): Kazuki Bando, Zhiqiang Zhang, Katsumasa Fujita and Satoshi Kawata
  • Organizer: SpringSciX
  • Int'l Joint Research
• [Presentation] Line-illumination Raman and SERS microscopy for live cell imaging (2018)
  • Author(s): Kazuki Bando, Zhiqiang Zhang, Kentaro Mochizuki, Kozue Watanabe, Nicholas I Smith, Satoshi Kawata, Katsumasa Fujita
  • Organizer: SpringSciX
  • Int'l Joint Research
• [Presentation] Au-coated Ag core/satellite nanoassemblies for SERS measurement in vivo (2018)
  • Author(s): Kazuki Bando, Zhiqiang Zhang, Kentaro Mochizuki, Katumasa Fujita and Satoshi Kawata
  • Organizer: 日本台湾ラマン分光学に関する国際シンポジウム(Japan/Taiwan International Symposium on Raman Spectroscopy)
  • Int'l Joint Research
• [Presentation] 表面増強ラマン散乱を用いた細胞内のpHセンシング (2018)
  • Author(s): 畔堂 一樹、張 志強、藤田 克昌、河田 聡
  • Organizer: バイオ関連シンポジウム
• [Presentation] SERS microscopy and metallic nanoparticle synthesis for living cell imaging (2018)
  • Author(s): Kazuki Bando, Katsumasa Fujita, and Satoshi Kawata
  • Organizer: 第三回フォトニクス研究会
  • Int'l Joint Research
• [Presentation] アルキンで標識した薬剤分子の細胞内3D表面増強ラマンイメージング (2018)
  • Author(s): 小池 康太, 安藤潤, 畔堂一樹, スミス ニコラス, 藤田 克昌
  • Organizer: 第16回医用分光学研究会
• [Presentation] Time-lapse 3D SERS imaging of alkyne-tagged drug molecules in live cells (2018)
  • Author(s): Kota Koike, Jun Ando, Kazuki Bando, Nicholas I. Smith, and Katsumasa Fujita
  • Organizer: The International Symposium for Materials Scientists Ⅲ
  • Int'l Joint Research
• [Presentation] Artificial cell reactor technology for digital bioassay, screening, and system reconstitution (2018)
  • Author(s): Hiroyuki Noji
  • Organizer: 1st Asian Synthetic Biology Association Meeting
  • Int'l Joint Research / Invited
• [Presentation] Remaining issues on F1-ATPase (2018)
  • Author(s): Hiroyuki Noji
  • Organizer: Japan-Sweden joint workshop on molecular motor
  • Int'l Joint Research / Invited
• [Presentation] Artificial cell reactor technology for directed evolution (2018)
  • Author(s): Hiroyuki Noji
  • Organizer: International Symposium on “Artificial Cell Reactor Science and Technology”
  • Int'l Joint Research / Invited
• [Presentation] Intrinsic diversities of catalytic activity among single enzyme molecules and virus particles revealed with femtoliter reactor array (2018)
  • Author(s): Hiroshi Ueno
  • Organizer: Joint meeting of the 10th International Forum on Post-Genomic Technology and the 11th International Workshop on Approaches to Single-Cell Analysis
  • Int'l Joint Research / Invited
• [Presentation] Molecular mechanism and robustness of Rotary catalysis of F1-ATPase (2018)
  • Author(s): Hiroyuki Noji
  • Organizer: Stockholm University Physics Seminar, Stockholm University
  • Int'l Joint Research / Invited
• [Presentation] Artificial cell reactor technology for directed evolution (2018)
  • Author(s): Hiroyuki Noji
  • Organizer: International Symposium on “Artificial cell reactor science and technology”
  • Int'l Joint Research / Invited
• [Presentation] 生体分子の構造ダイナミクス1分子計測法&微小リアクタを用いたデジタル分析技術 (2018)
  • Author(s): 野地博行
  • Organizer: 可視化ワークショップ
  • Invited
• [Presentation] 人工細胞リアクタとゲノム合成技術 (2018)
  • Author(s): 野地博行
  • Organizer: 文部科学省地域イノベーション・エコシステム形成プログラムバイオ経済を加速する革新技術ゲノム編集・合成技術の事業化キックオフシンポジウム
  • Invited
• [Presentation] 人工細胞リアクタを用いたバイオイノベーション創出の試み (2018)
  • Author(s): 野地博行
  • Organizer: 日立研究討論会
  • Invited
• [Presentation] Application for microchamber. (2018)
  • Author(s): 田端和仁
  • Organizer: JCVI seminar
  • Int'l Joint Research / Invited
• [Presentation] high sensitive measurement system and bacterium reconstitution by microchambe (2018)
  • Author(s): 田端和仁
  • Organizer: JSSB seminar
  • Int'l Joint Research / Invited
• [Presentation] 細胞内夾雑環境をマイクロデバイス内に作り込む (2018)
  • Author(s): 田端和仁
  • Organizer: 第18回日本蛋白質科学会年会
  • Invited
• [Presentation] 超解像イメージングとプラズモニクス、メタマテリアルズ (2018)
  • Author(s): 河田 聡
  • Organizer: 日本学術講演会メタマテリアル第187委員会
  • Invited
• [Presentation] Deep UV confocal Raman microscopy : label-free bio-molecular analytical imaging (2018)
  • Author(s): Satoshi Kawata
  • Organizer: Focus on Microscopy
  • Int'l Joint Research / Invited
• [Presentation] 3D and nano photonics (2018)
  • Author(s): Satoshi Kawata
  • Organizer: RCAS Seminar
  • Int'l Joint Research / Invited
• [Presentation] Alkyne-tag Raman imaging for small molecule imaging (2018)
  • Author(s): Katumasa Fujita
  • Organizer: International Conference of Applied Sciences 2018
  • Int'l Joint Research / Invited
• [Presentation] 細胞内輸送中の金ナノ粒子を用いた 表面増強ラマンイメージング (2018)
  • Author(s): 畔堂 一樹、安藤 潤、Nicholas I Smith、藤田 克昌、河田 聡
  • Organizer: 第43回レーザー顕微鏡研究会
• [Presentation] CHEMOMECHANICAL COUPLING OF ROTATION OF MAMMALIAN F1-ATPASE BY STATIC AND DYNAMIC X-RAY CRYSTALLOGRAPHIC STUDIES (2018)
  • Author(s): Toshiharu Suzuki
  • Organizer: Biophysical Society 62nd Annual Meeting(USA)
  • Int'l Joint Research
• [Presentation] 非線形光学効果を利用した超解像顕微鏡 (2017)
  • Author(s): 藤田克昌
  • Organizer: 分子科学研究所研究会
  • Place of Presentation: 岡崎
  • Year and Date: 2017-03-21
  • Invited
• [Presentation] ラマン散乱顕微鏡における超解像 (2017)
  • Author(s): 藤田克昌
  • Organizer: 第64回応用物理学会春季学術講演会
  • Place of Presentation: 横浜
  • Year and Date: 2017-03-15
  • Invited
• [Presentation] 超解像顕微鏡の原理と応用 (2017)
  • Author(s): 藤田克昌
  • Organizer: 日本オプトメカトロニクス協会 光センシング技術部会 研究会
  • Place of Presentation: 東京
  • Year and Date: 2017-02-28
  • Invited
• [Presentation] Raman Imaging of molecular dynamic during cellular events (2017)
  • Author(s): K. Fujita
  • Organizer: SPIE 2017 Nano-Bio Sensing, Imaging & Spectroscopy (Invited)
  • Place of Presentation: Jeju, Koria
  • Year and Date: 2017-02-22
  • Int'l Joint Research
• [Presentation] Super-resolution bioimaging by using optical nonlinearity (2017)
  • Author(s): K. Fujita
  • Organizer: The 17th RIES-Hokudai International Symposium (Invited)
  • Place of Presentation: Sapporo
  • Year and Date: 2017-02-15
  • Int'l Joint Research
• [Presentation] 非線形蛍光応答を利用した超解像顕微鏡 (2017)
  • Author(s): 藤田克昌
  • Organizer: 第11回基生研バイオイメージングフォーラム
  • Place of Presentation: 岡崎
  • Year and Date: 2017-02-15
  • Invited
• [Presentation] 近接場ナノ光学とプラズモニクス (2017)
  • Author(s): 河田 聡
  • Organizer: 東京大学生産技術研究所光電子融合研究センター 公開シンポジウム～光量子技術の新たな展開～
  • Invited
• [Presentation] Plasmonic growth of plasmonic metamaterials (2017)
  • Author(s): Satoshi Kawata
  • Organizer: AOM2017
  • Int'l Joint Research / Invited
• [Presentation] Deep UV Plasmonics and Raman Microscopy (2017)
  • Author(s): Satoshi Kawata
  • Organizer: Light Confernce 2017
  • Int'l Joint Research / Invited
• [Presentation] Alternative Methods in Optics (2017)
  • Author(s): Satoshi Kawata
  • Organizer: INP2017
  • Int'l Joint Research / Invited
• [Presentation] Deep UV Plasmonics and Raman Microscopy (2017)
  • Author(s): Satoshi Kawata
  • Organizer: Celebration of Nano-Optics
  • Int'l Joint Research / Invited
• [Presentation] Deep UV Plasmonics and resonant Raman scattering microscopy for analytical molecular imaging (2017)
  • Author(s): Satoshi Kawata
  • Organizer: OptoX NANO
  • Int'l Joint Research / Invited
• [Presentation] Plasmonics for bio-molecular nano-imaging (2017)
  • Author(s): Satoshi Kawata
  • Organizer: CUDOS workshop
  • Int'l Joint Research / Invited
• [Presentation] Raman microscopy beyond the limiot (2017)
  • Author(s): Katumasa Fujita
  • Organizer: The 42nd Seminar on RIKEN Center for Advanced Photonics
  • Int'l Joint Research / Invited
• [Presentation] Improvement of spatial and spectral resolution in Raman microscopy (2017)
  • Author(s): Katumasa Fujita
  • Organizer: Biomedical Imaging and Sensing Conference (BISC) 2017
  • Int'l Joint Research / Invited
• [Presentation] 限界を超えたバイオイメージング : 超解像顕微鏡の原理と応用 (2017)
  • Author(s): 藤田克昌
  • Organizer: OPIE17 セミナー
  • Invited
• [Presentation] Raman microscopy for molecular imaging of living cells (2017)
  • Author(s): Katumasa Fujita
  • Organizer: 5th International Symposium on Bioimaging (Joint symposium on Bioimagingbetween ingapore and Japan)
  • Int'l Joint Research / Invited
• [Presentation] Raman microscopy for molecular imaging of cells (2017)
  • Author(s): Katumasa Fujita
  • Organizer: RamanFest 2017
  • Int'l Joint Research / Invited
• [Presentation] Raman microscopy for molecular imaging of biological samples (2017)
  • Author(s): Katumasa Fujita
  • Organizer: ICAVS-9
  • Int'l Joint Research / Invited
• [Presentation] Raman microscopy : a new tool for molecular imaging of cells and tissues (2017)
  • Author(s): Katumasa Fujita
  • Organizer: Deciphering complex energy landscape and kinetic network from single molecules to cells : a new challenge to make theories meet experiments
  • Int'l Joint Research / Invited
• [Presentation] High speed Raman imaging for chemical profiling of cells and tissues (2017)
  • Author(s): Katumasa Fujita
  • Organizer: 生物物理学会
  • Int'l Joint Research
• [Presentation] The taming of the SERS (2017)
  • Author(s): Katumasa Fujita
  • Organizer: SCIX 2017
  • Int'l Joint Research / Invited
• [Presentation] SERS microscopy for investigation of drug dynamics in cellular and protein levels (2017)
  • Author(s): Katumasa Fujita
  • Organizer: Taiwan-Germany Workshop : Ultrafast laser spectroscopy and high-resolution optical imaging of plasmonics and molecular systems
  • Int'l Joint Research / Invited
• [Presentation] 無標識細胞分析 : ラマン分光イメージングの活用 (2017)
  • Author(s): 藤田克昌
  • Organizer: 関西再生医療産業コンソーシアム 第3回検討会「計測・検出」
  • Invited
• [Presentation] アルキンラマン顕微イメージングによる小分子検出 (2017)
  • Author(s): 藤田克昌
  • Organizer: 第15回医用分光学研究会
  • Invited
• [Presentation] 超解像バイオイメージングの原理と応用 (2017)
  • Author(s): 藤田克昌
  • Organizer: 第60回光波センシング技術研究会講演会
  • Invited
• [Presentation] Alkyne-tag Raman imaging for detecting small molecules in living cells (2017)
  • Author(s): Katumasa Fujita
  • Organizer: Global Nanophotonics 2017
  • Int'l Joint Research / Invited
• [Presentation] ラマン顕微鏡が拓く新しいバイオイメージング (2017)
  • Author(s): 藤田克昌
  • Organizer: 第7回オプトバイオシンポジウム
  • Invited
• [Presentation] アルミニウムナノ粒子を用いた深紫外第二高調波発生 (2017)
  • Author(s): 新田将、田口敦清、藤田克昌
  • Organizer: 応用物理学会関西支部平成29年度第1回講演会
• [Presentation] DUV plasmonics for SERS and TERS : materials and structures (2017)
  • Author(s): Atsushi Taguchi, Satoshi Kawata
  • Organizer: ICO-24
  • Int'l Joint Research
• [Presentation] 単一アルミニウムナノディスクからの深紫外第二高調波発生 (2017)
  • Author(s): 新田将、田口敦清、藤田克昌
  • Organizer: 第65回応用物理学会春季学術講演会
• [Presentation] Characterization of SERS spectra measured with gold nanoparticles during intracellular transportation. (2017)
  • Author(s): Kazuki Bando, Jun Ando, Nicholas I Smith, Katsumasa Fujita and Satoshi Kawata
  • Organizer: 分光学会年次講演会
  • Int'l Joint Research
• [Presentation] Dynamic SERS imaging for monitoring the intracellular chemical environment in 3D (2017)
  • Author(s): Kazuki Bando, Jun Ando, Nicholas I Smith, Katsumasa Fujita and Satoshi Kawata
  • Organizer: ICO-24
  • Int'l Joint Research
• [Presentation] Analytical characterization of intracellular dynamic enhanced Raman measurement during intracellular transportation (2017)
  • Author(s): Kazuki Bando, Jun Ando, Nicholas I Smith, Katsumasa Fujita and Satoshi Kawata
  • Organizer: INP2017
  • Int'l Joint Research
• [Presentation] Dynamic SERS imaging of intracellular environment (2017)
  • Author(s): Kazuki Bando, Jun Ando, Nicholas I Smith, Katsumasa Fujita and Satoshi Kawata
  • Organizer: SCIX 2017
  • Int'l Joint Research / Invited
• [Presentation] Quantitatively Evaluation of SERS Nanopartloies (2017)
  • Author(s): Zhiqiang Zhang, Kentaro Mochizuki, Kazuki Bando, Atsushi Taguchi, Katsumasa Fujita, Satoshi Kawata
  • Organizer: 分光学会年次講演会
  • Int'l Joint Research
• [Presentation] 角度分割・時分割X線結晶構造解析による、哺乳類F1-ATPaseのリン酸解離駆動の回転力発生機構の分析 (2017)
  • Author(s): 鈴木 俊治
  • Organizer: 第17回日本蛋白質科学会年会(仙台国際センター・仙台)
• [Presentation] Physics-Biology interface in the study on ATP synthase (2017)
  • Author(s): Hiroyuki Nojj
  • Organizer: 17th HFSP Awardees Meeting and The Physics of Living Matter for Tomorrow(Lisbon)
  • Int'l Joint Research / Invited
• [Presentation] Robustness of Chemo-mechanical Coupling of F1-ATPase Motor (2017)
  • Author(s): Hiroyuki Nojj
  • Organizer: First Conference on Biomotors, Virus Assembly, and Nanobiotechnology Applications(USA)
  • Int'l Joint Research / Invited
• [Presentation] 人工細胞デバイスを用いた蛋白質科学 (2017)
  • Author(s): 野地 博行
  • Organizer: 2017年度生命科学系学会合同年次大会(神戸)
  • Invited
• [Presentation] インフルエンザウィルス集団の少数性 (2017)
  • Author(s): 田端 和仁
  • Organizer: 2017年度生命科学系学会合同年次大会(神戸)
• [Presentation] 角度分割・時分割X線結晶構造解析による、哺乳類F1-ATPaseのリン酸解離駆動の回転力発生機構の分析 (2017)
  • Author(s): 鈴木 俊治
  • Organizer: 日本生物物理学会第55回年会(熊本大学)
• [Presentation] マイクロデバイスと大腸菌の融合を利用した新規人工細胞系の開発 (2017)
  • Author(s): 田端 和仁
  • Organizer: 日本生物物理学会第55回年会(熊本大学)
• [Presentation] 生体分子モーターが力を発生させているところを見る-時分割動的構造解析による哺乳類F1-ATPaseのリン酸解離駆動の回転力発生機構の分析- (2017)
  • Author(s): 鈴木 俊治
  • Organizer: 平成29年度日本結晶学会年会(広島)
• [Presentation] 分子モーターが動いている瞬間を見る : 時分割動的X線結晶構造解析による哺乳類F1の回転力発生機構の分析 (2017)
  • Author(s): 鈴木 俊治
  • Organizer: 2017年度生命科学系学会合同年次大会(神戸)
  • Invited
• [Presentation] X線結晶構造解析を駆使した、哺乳類F1-ATPaseの回転力発生機構の分析 (2017)
  • Author(s): 鈴木 俊治
  • Organizer: 日本生体エネルギー研究会 第43回討論会 2017京都(京都産業大学)
• [Presentation] Plasmonic Raman microscopy for nano, 3D, and deep UV imaging (2016)
  • Author(s): S. Kawata
  • Organizer: NANOP 2016(Invited)
  • Place of Presentation: University Pierre and Marie Curie, Paris, France
  • Year and Date: 2016-12-07
  • Int'l Joint Research
• [Presentation] Improvement of spatial-and temporal-resolution in Raman microscopy (2016)
  • Author(s): K. Fujita
  • Organizer: Japan-Taiwan Medical Specectroscopy Internatinonal Symposium (Invited)
  • Place of Presentation: Awaji Yumebutai, Hyogo
  • Year and Date: 2016-12-04
  • Int'l Joint Research
• [Presentation] ラマン散乱による細胞分析イメージング (2016)
  • Author(s): 安藤潤、藤田克昌
  • Organizer: 電子情報通信学会 第36回POC研究会
  • Place of Presentation: 大阪
  • Year and Date: 2016-11-30
  • Invited
• [Presentation] ラマン散乱を利用した高解像度イメージング (2016)
  • Author(s): 藤田克昌
  • Organizer: 日本光学会ナノオプティクス研究グループ第23回研究討論会
  • Place of Presentation: 大阪
  • Year and Date: 2016-11-29
  • Invited
• [Presentation] Raman spectroscopic approaches to label-free cell characterization and finding functional minorities (2016)
  • Author(s): K. Fujita
  • Organizer: The 54th Annual Meeting of the Biophysical Society of Japan(Invited)
  • Place of Presentation: Tukuba, Japan
  • Year and Date: 2016-11-25
  • Int'l Joint Research
• [Presentation] ラマン散乱を用いた高解像度分析イメージング (2016)
  • Author(s): 藤田克昌
  • Organizer: 第二回バイオナノフォトニクス研究会
  • Place of Presentation: 大阪
  • Year and Date: 2016-11-18
  • Invited
• [Presentation] ラマン分光顕微鏡とその生命科学への応用 (2016)
  • Author(s): 藤田克昌
  • Organizer: 光とレーザーの科学技術フェア2016
  • Place of Presentation: 東京
  • Year and Date: 2016-11-15
  • Invited
• [Presentation] Raman microcopy for imaging cellular duynamics (2016)
  • Author(s): K. Fujita
  • Organizer: 31st International Congress on High-Speed Imaging and Photoics (ICHSIP31)(Invited)
  • Place of Presentation: 大阪
  • Year and Date: 2016-11-08
  • Int'l Joint Research
• [Presentation] ラマン分光による顕微分析イメージング (2016)
  • Author(s): 藤田克昌
  • Organizer: 第二回産総研・阪大先端フォトニクス・バイオセンシングワークショップ
  • Place of Presentation: 大阪
  • Year and Date: 2016-10-28
  • Invited
• [Presentation] 超解像顕微鏡 : 光の限界をこえる (2016)
  • Author(s): 藤田克昌
  • Organizer: 第二回 Future of Radiology (Invited)
  • Place of Presentation: 東京
  • Year and Date: 2016-10-27
• [Presentation] ラマン散乱を用いた顕微分析イメージング (2016)
  • Author(s): 藤田克昌
  • Organizer: 高分子学会 高分子表面研究会
  • Place of Presentation: 東京
  • Year and Date: 2016-10-21
  • Invited
• [Presentation] ラマン散乱を用いた生体イメージング (2016)
  • Author(s): 安藤潤、藤田克昌
  • Organizer: 電気通信学会ソサイエティ大会
  • Place of Presentation: 札幌
  • Year and Date: 2016-09-21
  • Invited
• [Presentation] Raman microscopy beyond the limit (2016)
  • Author(s): K. Fujita
  • Organizer: SCIX(Invited)
  • Place of Presentation: Mineapolis, USA
  • Year and Date: 2016-09-19
  • Int'l Joint Research
• [Presentation] ラマン顕微鏡 : 分光分析とイメージングの融合 (2016)
  • Author(s): 藤田克昌
  • Organizer: 第四回バイオ関連化学シンポジウム若手フォーラム
  • Place of Presentation: 金沢
  • Year and Date: 2016-09-06
  • Invited
• [Presentation] 顕微鏡の限界を超える : 超解像顕微鏡とラマン散乱顕微鏡 (2016)
  • Author(s): 藤田克昌
  • Organizer: 組織細胞化学会総会
  • Place of Presentation: 東京
  • Year and Date: 2016-09-03
  • Invited
• [Presentation] ラマン散乱を用いた細胞分析イメージング (2016)
  • Author(s): 安藤潤、藤田克昌
  • Organizer: 京都バイオ計測センターシンポジウム
  • Place of Presentation: 京都
  • Year and Date: 2016-08-02
  • Invited
• [Presentation] Cellular imaging by DUV excitation through multiphoton process (2016)
  • Author(s): K. Fujita
  • Organizer: SPIE Optics + Photonics (Invited)
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-07-28
  • Int'l Joint Research
• [Presentation] Deep UV plasmonics and bio-photonics (2016)
  • Author(s): S. Kawata
  • Organizer: Theo Murphy International Scientifice Meeting New Horizons for Nanophotonics (Invited)
  • Place of Presentation: Kavli Royal Society Centre, Chicheley Hall, Buckinghamshire, UK
  • Year and Date: 2016-05-23
  • Int'l Joint Research
• [Presentation] 自発ラマン散乱を利用した細胞分子イメージング (2016)
  • Author(s): 藤田克昌
  • Organizer: ラマンバイオセンシングコンソーシアム
  • Place of Presentation: 東京
  • Year and Date: 2016-05-10
  • Invited
• [Presentation] ラマン散乱顕微鏡による細胞/組織の分子イメージング (2016)
  • Author(s): 藤田克昌
  • Organizer: 第55回生大医工学大会オーガナイズドセッション
  • Place of Presentation: 富山
  • Year and Date: 2016-04-28
• [Presentation] Superresolution Microscopy without Fluorescence (2016)
  • Author(s): S. -W. Chu, K. -Y. Li, P. -T. Shen, K. Fujita, S. Kawata
  • Organizer: Focus on Microscopy 2016
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2016-03-23
  • Description: 【発表確定】
  • Int'l Joint Research
• [Presentation] Raman tags : Nonfluorescent chemical probes for small molecules (2015)
  • Author(s): Katsumasa Fujita
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2015
  • Place of Presentation: Hawaii, U.S.
  • Year and Date: 2015-12-18
  • Description: 【発表確定】
  • Int'l Joint Research / Invited
• [Presentation] Visible-wavelength two-photon excitation of fluorcscent proteins for multicolor and high-resolution imaging (2015)
  • Author(s): K. Fujita, Y. Yamanaka, K. Saito, N. J. Smith, Y. Arai, K. Uegaki, Y. Yonemaru, K. Mochizuki, S. Kawata, T. Nagai
  • Organizer: The international chemical congress of pacific basin societies 2015
  • Place of Presentation: Hawaii, U.S.
  • Year and Date: 2015-12-16
  • Description: 【発表確定】
  • Int'l Joint Research
• [Presentation] Single molecule analysis of transporter protein with arrayed lipid bilayer chamber (2015)
  • Author(s): Hiroyuki Noji
  • Organizer: The international chemical congress of pacific basin societies 2015
  • Place of Presentation: Hawaii, US
  • Year and Date: 2015-12-15
  • Description: 【発表確定】
  • Int'l Joint Research / Invited
• [Presentation] Redesigning of F1-ATPase (2015)
  • Author(s): Hiroyuki Noji
  • Organizer: The international chemical congress of pacific basin societies 2015
  • Place of Presentation: Hawaii, US
  • Year and Date: 2015-12-15
  • Description: 【発表確定】
  • Int'l Joint Research / Invited
• [Presentation] Raman microscopy using alkyne tag for small molccule imaging (2015)
  • Author(s): Katsumasa Fujita
  • Organizer: The 7th Asia and Occania Conference on Photobiology
  • Place of Presentation: Taipei. Taiwan
  • Year and Date: 2015-11-17
  • Description: 【発表確定】
  • Int'l Joint Research / Invited
• [Presentation] Alkyne-tag Raman imaging of bio-active small molccules in live cells (2015)
  • Author(s): J. Ando, A. F. Palonpon, K. Dodo, S. Kawata, M. Sodcoka, K. Fujita
  • Organizer: SPIE/OSJ Biophotonics Japan 2015
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2015-10-27
  • Description: 【発表確定】
  • Int'l Joint Research
• [Presentation] Surface-enhanced Raman scattering (SERS) spectroscopy for intracellular chemical imaging and protein analysis (2015)
  • Author(s): Katsumasa Fujita
  • Organizer: The Great Scientific Exchange 2015
  • Place of Presentation: Providence, U.S.
  • Year and Date: 2015-09-28
  • Description: 【発表確定】
  • Int'l Joint Research / Invited
• [Presentation] Single molccule biophysics of ATP synthase and digitalization revolution of bioassay (2015)
  • Author(s): Hiroyuki Noji
  • Organizer: POSTECH seminar
  • Place of Presentation: Pohang University of Science and Technology, Korea
  • Year and Date: 2015-09-24
  • Description: 【発表確定】
  • Int'l Joint Research / Invited
• [Presentation] Lanthanide ions for suppressing photodegradation of biological cells under deep-ultrnviolet light exposure (2015)
  • Author(s): Yasuaki Kumamoto, Katsumasa Fujita, Nicholas Smith, Satoshi Kawata
  • Organizer: JSAP-OSA Joint Symposia 2015
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2015-09-16
  • Description: 【発表確定】
  • Int'l Joint Research
• [Presentation] Visible-wavelength two-photon excitation microscopy (2015)
  • Author(s): Katsumasa Fujita
  • Organizer: The 11th Conference on Lasers and Electro-Optics Pacific Rim
  • Place of Presentation: Busan, Korea
  • Year and Date: 2015-08-25
  • Description: 【発表確定】
  • Int'l Joint Research / Invited
• [Presentation] Visible-wavelength two-photon excitation microscopy (2015)
  • Author(s): Katsumasa Fujita
  • Organizer: SPIE Optics+Photonics
  • Place of Presentation: San Diogo, U.S.
  • Year and Date: 2015-08-10
  • Description: 【発表確定】
  • Int'l Joint Research / Invited
• [Presentation] Plasmonics : an exotic nanophotonics beyond the limits (2015)
  • Author(s): Satoshi Kawata
  • Organizer: International Nano Optoelectronics Workshop 2015
  • Place of Presentation: 東京工業大学 大岡山キャンパス、東京
  • Year and Date: 2015-08-06
  • Description: 【発表確定】
  • Int'l Joint Research / Invited
• [Presentation] 細胞および分子の化学状態の可視化 : ラマン顕微鏡の活用 (2015)
  • Author(s): 藤田 克昌
  • Organizer: 第67回日本細胞生物学会大会
  • Place of Presentation: タワーホール船堀、東京
  • Year and Date: 2015-07-02
  • Description: 【発表確定】
  • Invited
• [Presentation] High-resolution Raman microscopy for molecular imaging of cells (2015)
  • Author(s): Katsumasa Fujita
  • Organizer: The 20th OptoElectronics and Communications Conference
  • Place of Presentation: Shanghai, China
  • Year and Date: 2015-06-29
  • Description: 【発表確定】
  • Int'l Joint Research / Invited
• [Presentation] ラマン分光イメージング (2015)
  • Author(s): 藤田克昌
  • Organizer: オブトロニクス特別セミナー
  • Place of Presentation: 東京
  • Year and Date: 2015-06-26
  • Description: 【発表確定】
  • Invited
• [Presentation] Plasmonies : an exotics nanophotonics beyond the classical limits (2015)
  • Author(s): Satoshi Kawata
  • Organizer: The 5th International Symposium on Organic and Inorganic Electronic Materials and Related Nanotechnologies
  • Place of Presentation: 朱鷺メッセ 新潟コンベンションセンター、新潟
  • Year and Date: 2015-06-17
  • Description: 【発表確定】
  • Int'l Joint Research / Invited
• [Presentation] 超解像蛍光顕微鏡の原理と応用 (2015)
  • Author(s): 藤田 克昌
  • Organizer: 第26回阪大医療組織工学フォーラム
  • Place of Presentation: 大阪大学 吹田キャンパス、大阪
  • Year and Date: 2015-06-08
  • Description: 【発表確定】
  • Invited
• [Presentation] Molecular imaging of cells by Raman scattering (2015)
  • Author(s): Katsumasa Fujita
  • Organizer: Advanced Microscopy Meeting - Super Resolution in Different Dimensions 2015
  • Place of Presentation: Moscow, Russia
  • Year and Date: 2015-06-02
  • Description: 【発表確定】
  • Int'l Joint Research / Invited
• [Presentation] Molecular imaging of cells by using Raman scattering (2015)
  • Author(s): 藤田 克昌
  • Organizer: 第10回日本分子イメージング学会 学術集会
  • Place of Presentation: タワーホール船堀、東京
  • Year and Date: 2015-05-20
  • Description: 【発表確定】
  • Invited
• [Presentation] Plasmonics : oxotic nanophotonics beyond the classical limits in optics (2015)
  • Author(s): Satoshi Kawata
  • Organizer: Applied Optics and Photonics, China 2015
  • Place of Presentation: Beijing, China
  • Year and Date: 2015-05-06
  • Description: 【発表確定】
  • Int'l Joint Research / Invited
• [Presentation] Raman microscopy for molecular imaging of cells (2015)
  • Author(s): Katsumasa Fujita
  • Organizer: Applied Optics and Photonics, China 2015
  • Place of Presentation: Beijing, China
  • Year and Date: 2015-05-06
  • Description: 【発表確定】
  • Int'l Joint Research / Invited
• [Presentation] Visible-wavelength two-photon excitation microscopy (2015)
  • Author(s): K. Fujita, M. Yamanaka, K. Uegaki, K. Saito, Y. Arai, Y. Yonemaru, K. Mochizuki, S. Nicholas, S. Kawata, T. Nagai
  • Organizer: 5th Asian and Pacific-Rim Symposium on Biophotonics
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2015-04-24
  • Description: 【発表確定】
  • Int'l Joint Research
• [Presentation] Resolution and Enhancement in TERS Microscopy (2014)
  • Author(s): Satoshi Kawata
  • Organizer: 4th International Conference on Tip Enhanced Raman Spectroscopy
  • Place of Presentation: Rio De Janeiro, Brazil
  • Year and Date: 2014-09-09
  • Invited
• [Presentation] Plasmonic Super-resolution Imaging beyond the Plasmonic Limit (2014)
  • Author(s): Satoshi Kawata
  • Organizer: Progress In Electromagnetics Research Symposium
  • Place of Presentation: Langham Place, Guangzhou, Chaina
  • Year and Date: 2014-08-27
• [Presentation] 3D plasmonic nano imaging and spectroscopy of a living cell with a gold nanoparticle (2014)
  • Author(s): Satoshi Kawata
  • Organizer: SPIE Optics&Photonics 2014
  • Place of Presentation: San Diego Convention Center, USA
  • Year and Date: 2014-08-17
  • Invited
• [Presentation] Plasmonic self-growth of plasmonic 3D nanostructures
  • Author(s): 河田聡
  • Organizer: 40th PIERS 2018
  • Int'l Joint Research / Invited
• [Book] prehensive Nanoscience and Nanotechnology, 2e. Volume 4 : Spectroscopy and Microsc (2019)
  • Author(s): Satoshi Kawata ed.
  • Total Pages: 360
  • Publisher: Elsevier
• [Remarks] 有限会社セレンディップ研究所
  • URL: https://serendip.themedia.jp/
• [Remarks] 東京大学・野地研究室
  • URL: http://www.nojilab.t.u-tokyo.ac.jp/
• [Remarks] 大阪大学・藤田研究室
  • URL: https://lasie.ap.eng.osaka-u.ac.jp/home_j.html