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プロテアソーム:動作原理の解明と生理病態学研究

Research Project

Project/Area Number 26000014
Research Category

Grant-in-Aid for Specially Promoted Research

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionTokyo Metropolitan Institute of Medical Science

Principal Investigator

田中 啓二  公益財団法人東京都医学総合研究所, 生体分子先端研究分野, 所長 (10108871)

Co-Investigator(Kenkyū-buntansha) 村田 茂穂  東京大学, 大学院薬学研究科, 教授 (20344070)
Project Period (FY) 2014 – 2018
Project Status Granted (Fiscal Year 2017)
Budget Amount *help
¥406,640,000 (Direct Cost: ¥312,800,000、Indirect Cost: ¥93,840,000)
Fiscal Year 2018: ¥81,770,000 (Direct Cost: ¥62,900,000、Indirect Cost: ¥18,870,000)
Fiscal Year 2017: ¥77,610,000 (Direct Cost: ¥59,700,000、Indirect Cost: ¥17,910,000)
Fiscal Year 2016: ¥79,170,000 (Direct Cost: ¥60,900,000、Indirect Cost: ¥18,270,000)
Fiscal Year 2015: ¥81,770,000 (Direct Cost: ¥62,900,000、Indirect Cost: ¥18,870,000)
Fiscal Year 2014: ¥86,320,000 (Direct Cost: ¥66,400,000、Indirect Cost: ¥19,920,000)
Keywords蛋白質 / バイオテクノロジー / プロテオーム / 脳神経疾患 / 免疫学
Outline of Annual Research Achievements

「プロテアソームに関する包括的基盤研究」
プロテアソームによるタンパク質分解の主要経路の発見(Mol Cell 2017)。
ユビキチン化タンパク質がユビキチンレセプター(Rpn10とRpn13)を介して直接プロテアソームで分解される経路は10%以下であり、細胞内ではUBL-UBAシャトル分子(Rad23やDsk2)が基質を捕捉してプロテアソームに運搬、分解する間接的な経路が90%以上を占めることを証明した。
・ユビキチンK48-K63分岐鎖の役割を解明(PNAS 2018)。
K48-K63分岐型ユビキチン鎖が、プロテアソーム依存性のタンパク質分解シグナルとして作用することを見出した。
・ユビキチン鎖の長さの解明(Nat Commun 2018)。
細胞内のユビキチン鎖の長さを測定する手法を開発し、Ub-ProT法と命名、細胞内のタンパク質に付加したユビキチン鎖の長さが通常2~7重鎖であることを明らかにした。
「生理病態学1 : 分子病態研究」
「パーキンソン病に関する研究」(Elife 2018)。
Pink-Parkin依存的に不良ミトコンドリアが分解されるパスウェイにおいて、Rab(GTP-GDP)サイクルが関係していることを見出した。
家族性パーキンソン病の責任遺伝子DJ-1の機能解析(Sci Rep 2017)。
遺伝性のパーキンソン病に関係するDJ-1タンパク質が、アルデヒドの解毒を介してミトコンドリアの機能を助けることで、パーキンソン病の発症を抑えていることを明らかにした。
「生理病態学2 : 分子免疫研究」
胸腺プロテアソーム遺伝子β5tのcTEC特異的発現機構(Nat. Commun. 2017)。
β5tは胸腺皮質の上皮性細胞に発現している。本年度、転写因子Foxnlがβ5tの胸腺特異的な発現に関わる転写因子であることを明らかにした。
胸腺プロテアソームのヒトSNP解析によるキラーT細胞のレパトア形成(JCI Insight 2017)。
β5tのSNPを調べたところ、Human G49A variation : SNP rs34457782(rs reference SNP ID number)変異が高頻度に起きていることを見出し、そのレパトア形成における意義をβ5t-G49S-knock-in miceを作出して解析した。

Current Status of Research Progress
Current Status of Research Progress

1: Research has progressed more than it was originally planned.

Reason

大きな研究成果を挙げ、多くの論文として発表した。
「プロテアソームに関する包括的基盤研究」
・マウス肝臓でプロテアソームを欠損させると、オートファジーが強く誘導され得ることを見出した(J Biol Chem 2014)。
・出芽酵母26Sプロテアソームの細胞内動態をQuantitative live-cell imaging法で解明した(Nat. Commun. 2014)。
・26Sプロテアソームの分子集合(形成)に関わるRPシャペロンであるNas2の立体構造の解明に成功した(Structure 2014)。
・ポリユビキチン鎖が構造不安性を増幅して、基質を凝集させること、そしてオートファジーで処理されることを見出した(Nat Commun 2015)。
・TR-TUBE(トリプシン耐性のユビキ鎖に高い親和性を示すUBAプロープ)を開発して、ユビキチン化基質の同定方法を確立した(PNAS 2015)。
・アセチル化ユビキチンの同定とユビキチン鎖の伸長制御における機能の解析に成功した(EMBO Rep 2015)。
・K48-K63分岐鎖を同定し、この分岐鎖がNF-・Bのシグナルにおいて重要な役割を果たしていることを見出した(Mol Cell 2016)。
・26Sプロテアソームの分子集合シャペロンPAC4の立体構造解析に成功した(Protein Sci 2017)。
・細胞内のユビキチン鎖の長さを決定する方法を開発し、Ub-ProT法と命名した(Nat Commun 2018)。
・K48-K63分岐鎖がプロテアソーム分解に関係していることを、世界で初めて突き止めた(PNAS 2018)。
「生理病態学1 : 分子病態研究」
・ユビキチンのリン酸化がParkinの活性化を誘導し、Mitophagy(不良ミトコンドリアの選択的オートファジー)を引き起こすことを見出した(Nature 2014)。
・p62/SqstmlがNrf2-依存的に代謝を再編して、肝細胞がんを誘発する仕組みを解明した(Nat Commun 2015)。
・脱ユビキチン酵素USP8が変異すると、Cushing's diseaseを引き起こすことを見出した(Nat Genet 2015)。
・Parkinの活性化機構をリン酸化ユビキチンのSite-specific interaction mapping法で解明した(J Biol Chem 2015)。
・Pink1が損傷ミトコンドリア外膜に蓄積するメカニズムを解明した(J Cell Sci 2015)。
・パーキンソン病の原因遺伝子産物DJ-1がアルデヒドを酸化する解毒酵素として作用することを見出した(Sci Rep 2017)。
・ミトコンドリア外膜に局在するUsp30の構造解析に成功し、Mitophagyの可逆的な制御機構を解明した(Nat Struct Mol Biol 2017)。
・リソファジーに関係する糖鎖識別ユビキチンリガーゼ(SCFFBX027)の同定に成功した(PNAS 2017)。
・ドーパミンニューロン特異的にオートファジーを欠損させた老齢マウスの神経細胞においてレヴィー小体(封入体)が出現し、モータータンパク質の機能が不全になることを突き止めた(Sci Rep 2018)。
・Pink-Parkin依存的に不良ミトコンドリアが分解されるパスウェイにおけるRabサイクルのMitophagyにおける役割を解明した(Elife 2018)。
「生理病態学2 : 分子免疫研究」
・胸腺プロテアソームが正の選択を誘導する分子メカニズムの解明に成功した。(Nat Commun 2015)。
・免疫(β1i, β2i, β5i)及び胸腺プロテアソームβ5tの同時欠損マウスを作製して、胸腺の正の選択に関わる機構について考察した(Nat Immunol 2015)。
・胸腺プロテアソームβ5tのcTEC特異的な発現調節機構の解明に成功した(Nat Commun 2017)。
・胸腺プロテアソームのヒトSNP解析によるVariationとキラーT細胞のレパトア形成について解析した(JCI Insight 2017)。

Strategy for Future Research Activity

「プロテアソームに関する包括的基盤研究」
研究開始後、プロテアソームの分解シグナルであるユビキチン鎖の多様性について、高感度質量分析計を駆使して解析し、多くの成果を挙げてきた。しかしながら多様なユビキチン鎖の中で、分岐鎖や混合鎖については、我々が明らかにしてきたK48-K63鎖の機能(Mol Cell 2016, PNAS 2018)以外には、殆どが不明である。そこで今後、Multiple monoユビキチン修飾を含めて、どのような修飾系がプロテアソームの分解シグナルとして機能するかについて、詳細な解析を進めて行く計画である。
もう一つは、核におけるタンパク質の分解、特にタンパク質のリサイクリング(品質管理)についての解明である。我々は、既に多くのがん細胞や活発に増殖している細胞では、核内にタンパク質が濃縮しており、その絶対量は1・Mと細胞質(200 nM)の5倍以上であることを蛍光相関分光法(fluorescence correlation spectroscopy : FCS)や蛍光相互相関分光法(fluorescence cross-correlation spectroscopy : FCCS)を駆使して明らかにしてきた(Nat Commun 2014)。しかしながら核プロテアソームの実態解明は、その多くが不明である。我々は、この解明に資するヒントをごく最近、見出した。それは、外環境ストレス(高浸透圧処理)に応答したプロテアソームの新しい時空間的な(spatio-temporal)防御応答機構を発見したことである。即ち、プロテアソームの異なる構成サブユニットにEGFPあるいはFusionRedをノックインしたヒト培養細胞(HCT116またはhTERT-RPE1)を作製し、長時間タイムラプスあるいは蛍光相関分光法によりプロテアソームの細胞内動態を解析したところ、プロテアソームは間期においては核にenrichしているが分裂期では核膜の崩壊とともに細胞中に分散し、終期の核膜再生に伴いプロテアソームが核に集積することを見出した。また、プロテアソームは細胞内の環境ストレスに応答して様々に局在を変えること(核-細胞質振動)が明らかとなった。高浸透圧刺激時、プロテアソームは数秒で核内にfociを形成し、その後徐々に消失する。その後の研究から、この核内のFociはLiquid- … More phase Separationと同じような挙動をし、核質で変性したユビキチン化されたリボソームタンパク質が主成分であることを突き止めた。これは核にタンパク質の品質管理機構が存在することを示唆しており、複数のユビキチン連結酵素(E3リガーゼ)が関与している可能性が考えられた。それらを同定して、核におけるプロテアソームの役割、そして核内の品質管理機構を明らかにして行く計画である。
「生理病態学1 : 分子病態研究」
Pink1-Parkin依存性のMitophagyについて不良ミトコンドリアのユビキチン化識別以降のプロセスが不明であるので、この点に集中して解析を進める計画である。とくに我々が見出したRab(GTP-GDP)サイクルがどのようにしてMitophagyの制御に関与しているのかについても詳しい解析を進める計画である。もう一つのDJ-1については、我々が見出したアルデヒド酸化酵素としての解毒機能が、実際にミトコンドリアの品質管理、引いてはパーキンソン病の発症まで関係するのかについて、すでにin vivo(ハエを用いた遺伝学研究)を開始しており、その結果を得て、マウスの遺伝学的解析に適用することを計画中である。
「生理病態学2 : 分子免疫研究」
胸腺プロテアソームを発現するcTECはCD8+T細胞の「正の選択 : Positive Selection」を行うことできるが、これまでに、そのレパトア形成の基本原理を提案した。しかしまだ残された多くの課題がある。例えば、胸腺プロテアソームによる正の選択を誘導するペプチドの同定は、本仮説を証明する核心であるが、未だ成功していない。これは胸腺に存在するcTEC細胞の量が少なく最先端のLC-MS/MSでも正の選択を誘導するペプチドの同定が困難なためである。そこで、胸腺が野生型より約5倍大きな変異マウスとの交配を行い、大量のcTEC細胞を分離、そのMHC-class I会合ペプチドの分離を目指す。予備的であるが、すでに興味深い知見が集積しつつあり、本課題は「自己と非自己の識別機構」解明という免疫学における根源的テーマとして、意欲的に解析してゆきたい。
有顎脊椎動物から二つの免疫型(免疫及び胸腺)プロテアソームの遺伝子は、存在するが、進化系統樹を詳細に辿ると、驚いたことに、調べた限り鳥類のみ、MHCやTAP輸送系など適応免疫に関係した多くの遺伝子群は他の生物と同様に存在するが、不思議なことに免疫型遺伝子は存在しなかった。鳥類のT細胞レパトア形成はどうなっているのであろうか? 全く未知なT細胞のレバトア形成機構が存在するのか、あるいは、「正の選択(positive selection : 有用なT細胞の生存)」がなく「負の選択(negative selection : 自己と反応する有害な細胞の除去)」のみでレパトア形成がなされているのか、興味が尽きない課題である。しかし、自己と非自己の識別という観点からは、免疫の基本的な問題であり、研究試料を取集し、この課題に取り組んで行く計画である。 Less

Assessment Rating
Result (Rating)

A: Progress in the research is steadily towards the initial goal. Expected research results are expected.

Report

(9 results)
  • 2017 Annual Research Report   Abstract(Research Progress Assessment) ( PDF )   Research Progress Assessment (Result) ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report   Research Progress Assessment (Comments on the Site Visits) ( PDF )
  • 2014 Abstract ( PDF )   Comments on the Screening Results ( PDF )   Annual Research Report

Research Products

(163 results)

All 2018 2017 2016 2015 2014 Other

All Int'l Joint Research Journal Article Presentation Book Remarks Patent(Industrial Property Rights)

  • [Int'l Joint Research] マサチューセッツ大学(アメリカ合衆国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] マサチューセッツ大学/シンシナティ小児病院/NIH(アメリカ合衆国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Ottawa Hospital Research Institute(カナダ)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] K63 ubiquitylation triggers proteasomal degradation by seeding branched ubiquitin chains.2018

    • Author(s)
      Ohtake, F., Tsuchiya, H., Saeki, Y., and Tanaka, K.
    • Journal Title

      Proc. Natl. Acad. Sci. USA.

      Volume: 115 Pages: 1401-1408

    • DOI
      10.1073/pnas.1716673115
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ub-ProT reveals global length and composition of protein ubiquitylation in cells.2018

    • Author(s)
      Tsuchiya, H., Burana, D., Ohtake, F., Arai, N., Kaiho, A., Komada, M., and Tanaka, K.*, and Saeki, Y. * (*Correspondence)
    • Journal Title

      Nat. Commun.

      Volume: 9 Pages: 524-524

    • DOI
      10.1038/s41467-018-02869-x
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Endosomal Rab cycles regulate Parkin-mediated mitophagy.2018

    • Author(s)
      Yamano, K., Wang, C., Sarraf, S. A., Munch, C., Kikuchi, R., Noda, N. N., Hizukuri, Y., Kanemaki, M. T., Harper, W., Tanaka, K., Matsuda, N., and Youle, R. J.
    • Journal Title

      Elife

      Volume: 7

    • DOI
      10.7554/elife.31326
    • NAID
      120006457172
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Loss of autophagy in dopaminergic neurons causes Lewy pathology and motor dysfunction in aged mice.2018

    • Author(s)
      Sato, S., Uchihara, T., Fukuda, T., Noda, S., Kondo, H., Saiki, S., Komatsu, M., Uchiyama, Y., Tanaka, K., and Hattori, N.
    • Journal Title

      Sci. Rep.

      Volume: 8 Pages: 2813-2813

    • DOI
      10.1038/s41598-018-21325-w
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Accelerated invagination of vacuoles as a stress response in chronically heat-stressed yeasts.2018

    • Author(s)
      Ishii, A., Kawai, K., Noda, H., Kato, H., Takeda, K., Asakawa, K., Ichikawa, Y., Sasanami, T., Tanaka, K., and Kimura, Y.
    • Journal Title

      Sci Rep.

      Volume: 8 Pages: 2-644

    • DOI
      10.1038/s41598-018-20781-8
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reactive oxygen species up-regulate expression of muscle atrophy-associated ubiquitin ligase Cbl-b in rat L6 skeletal muscle cells.2018

    • Author(s)
      Uchida, T., Sakashita, Y., Kitahata, K., Yamashita, Y., Tomida, C., Kimori, Y., Komatsu, A., Hirasaka, K., Ohno, A., Nakao, R., Higashitani, A., Higashibata, A., Ishioka, N., Shimazu, T., Kobayashi, T., Okumura, Y., Choi, I., Oarada, M., Mills, E. M., Teshima-Kondo, S., Takeda, S., Tanaka, E., Tanaka, K., Sokabe, M., and Nikawa, T.
    • Journal Title

      Am J Physiol Cell Physiol.

      Volume: 印刷中

    • DOI
      10.1152/ajpcell.00184.2017
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cytosolic N-Glycans : Triggers for Ubiquitination Directing Proleasomal and Autophagic Degradation : Molecular Systems for Monitoring Cytosolic N-Glycans as Signals for Unwanted Proteins and Organelles.2018

    • Author(s)
      Yoshida, Y. and Tanaka, K.
    • Journal Title

      BioEssays

      Volume: 40

    • DOI
      10.1002/bies.201700215
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylated ubiquitin chain is the genuine Parkin receptor2018

    • Author(s)
      Okatsu, K., Koyano, F., Kimura, M., Kosako, H., Saeki, Y., Tanaka, K. * and Matsuda, N. * (*cocorespondences)
    • Journal Title

      J Pharm Biomed Anal.

      Volume: 209 Pages: 111-128

    • DOI
      10.1083/jcb.201410050
    • Related Report
      2017 Annual Research Report 2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A novel approach to assess the ubiquitin-modifier 1 (UFM1) system in cells.2017

    • Author(s)
      Ishimura, R., Obata, M., Kageyama, S., Daniel, J., Tanaka, K., and Komatsu, M.
    • Journal Title

      FEBS Lett.

      Volume: 591 Pages: 196-204

    • DOI
      10.1002/1873-3468.12518
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Foxnl-β5t transcriptional axis controls CD8+T cell production in the thymus.2017

    • Author(s)
      Uddin, MM., Ohigashi, I., Motosugi, R., Nakayama, T., Sakata, M., Hamazaki, J., Nishito, Y., Rode, I., Tanaka, K., Takemoto, T., Murata, S., and Takahama, Y.
    • Journal Title

      Nat. Commun.

      Volume: 8 Pages: 1-10

    • DOI
      10.1038/ncomms14419
    • NAID
      120006306633
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Crystal structure of human proteasome assembly chaperone PAC4 involved in proteasome formation.2017

    • Author(s)
      Kurimoto, E., Satoh, T., Ito, Y., Ishihara, E., Okamoto, K., Yagi-Utsumi, M., Tanaka, K., and Kato, K.
    • Journal Title

      Protein Sci.

      Volume: 26 Pages: 1080-1085

    • DOI
      10.1002/pro.3153
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] In Vivo Ubiquitin Linkage-type Analysis Reveals that the Cdc48-Rad23/Dsk2 Axis Contributes to K48-linked Chain Specificity of the Proteasome.2017

    • Author(s)
      Tsuchiya, H., Ohtake, F., Arai, N., Kaiho, A., Yasuda, S., Tanaka, K. *, and Saeki, Y. * (*Correspondence)
    • Journal Title

      Mol Cell

      Volume: 66 Pages: 488-502

    • DOI
      10.1016/j.molcel.2017.04.024
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A human Psmbll variant affects molecular processing of thymoproteasome and thymic production of CD8+ T cells.2017

    • Author(s)
      Ohigashi, I., Ohte, Y., Setoh, K., Nakase, H., Maekawa, A., Kiyonari, H., Hamazaki, Y., Sekai, M., Sudo, T., Tabara, Y., Sawai, H., Omae, Y., Yuliwulandari, R., Tanaka, Y., Mizokami, M., Inoue, H., Kasahara, M., Minato, N., Tokunaga, K., Tanaka, K., Matsuda, F., Murata, S., and Takahama, Y.
    • Journal Title

      JCI Insight

      Volume: 2 Pages: 1-14

    • DOI
      10.1172/jci.insight.93664
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Ubiquitination of stalled ribosome triggers ribosome-associated quality control.2017

    • Author(s)
      Matsuo, Y., Ikeuchi, K., Saeki, Y., Iwasaki, S., Schmidt, C., Udagawa, T., Sato, F., Tsuchiya, T., Becker, T., Tanaka, K., Ingolia, N. T., Beckmann, R., and Inada, T.
    • Journal Title

      Nat. Commun.

      Volume: 8 Pages: 159-159

    • DOI
      10.1038/s41467-017-00188-1
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Inhibitory effects of local anesthetics on the proteasome and their biological actions.2017

    • Author(s)
      Bahrudin, U., Unno, M., Nishio, K., Kita, A., Li, P., Kato, M., Inoue, M., Tsujitani, S., Murakami, T., Sugiyama, R., Saeki, Y., Obara, Y., Tanaka, K., Yamaguchi, H., Sakane, I., Kawata, Y., Itoh, T., Ninomiya, H., Hisatome, I., and Morimoto, Y.
    • Journal Title

      Sci. Rep.

      Volume: 7 Pages: 5-079

    • DOI
      10.1038/s41598-017-04652-2
    • NAID
      120006364094
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Purkinje cells are more vulnerable to the specific dcpletion of cathcpsin D than to that of atg72017

    • Author(s)
      Koike M, Shinbata M, Sunabori T, Yamaguchi J, Sakimura K, Komatsu M, Tanaka K, Uchiyama Y.
    • Journal Title

      Am. J. Pathol.

      Volume: 187 Issue: 7 Pages: 1586-1600

    • DOI
      10.1016/j.ajpath.2017.02.020
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Atg9a deficiency causes axon-specific lesions including neuronal circuit dysgenesis.2017

    • Author(s)
      Yamaguchi J, Suzuki C, Nanao T, Kakuta S, Ozawa K, Tanida I, Saitoh T, Sunabori T, Komatsu M, Tanaka K, Aoki S, Sakimura K, Uchiyama Y.
    • Journal Title

      Autophagy

      Volume: 17 Pages: 43-114

    • DOI
      10.1080/15548627.2017.1314897
    • Description
      in press
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Ubiquitination of exposed glycoproteins by SCFFBX027 directs damaged lysosomes for autophagy.2017

    • Author(s)
      Yoshida, Y., Yasuda, S., Fujita, T., Hamasaki, M., Murakami, A., Kawawaki, J., Iwai, K., Saeki, Y., Yoshimori, T., Matsuda, N., and Tanaka, K
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 114 Pages: 8574-8579

    • DOI
      10.1073/pnas.1702615114
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Structural basis for specific cleavage of Lys6-linked polyubiquitin chains by USP30.2017

    • Author(s)
      Sato, S., Okatsu, K., Saeki, Y., Yamano, K., Matsuda, M., Kaiho, A., Yamagata, A., Goto-Ito, S., Ishikawa, M., Hashimoto, Y., Tanaka, K., and Fukai, S.
    • Journal Title

      Nat. Struct. Mol. Biol.

      Volume: 24 Pages: 911-919

    • DOI
      10.1038/nsmb.3469
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Parkinson's disease-related DJ-1 functions in thiol quality control against aldehyde attack in vitro.2017

    • Author(s)
      Matsuda, N., Kimura., M., Queliconi, B. B., Kojima, W., Mishima, M., Takagi, K., Koyano, F., Yamano, K., Mizushima, T., Ito, Y., and Tanaka, K.
    • Journal Title

      Sci Rep.

      Volume: 7 Pages: 12816-12816

    • DOI
      10.1038/s41598-017-13146-0
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structure of the Dnmtl Reader Module Complexed with a Unique Two-Mono-Ubiquitin Mark on Histone H3 Reveals the Basis for DNA Methylation Maintenance.2017

    • Author(s)
      Ishiyama, S., Nishiyama, A., Saeki, Y., Moritsugu, K., Morimoto, D., Yamaguchi, L., Arai, N., Matsumura, R., Kawakami, T., Mishima, Y., Hojo, H., Shimamura. S., Ishikawa, F., Tajima, S., Tanaka, K., Ariyoshi, M., Shirakawa, M., Ikeguchi, M., Kidera, A., Suetake, I., Arita, K., and Nakanishi, M.
    • Journal Title

      Mol. Cell

      Volume: 68 Pages: 350-360

    • DOI
      10.1016/j.molcel.2017.09.037
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Early and consistent overexpression of ADRM1 in ovarian high-grade serous carcinoma.2017

    • Author(s)
      Jiang RT, Yemelyanova A, Xing D, Anchoori RK, Hamazaki J, Murata S, Seidman JD, Wang TL, Roden RBS.
    • Journal Title

      J Ovarian Res

      Volume: 10 Pages: 53-53

    • DOI
      10.1186/s13048-017-0347-y
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Human thymoproteasome variations influence CD8 T cell selection.2017

    • Author(s)
      Takeshi Nitta, Yuta Kochi, Ryunosuke Muro, Yoshihiko Tomofuji, Tadashi Okamura, Shigeo Murata, Harumi Suzuki, Takayuki Sumida, Kazuhiko Yamamoto, Hiroshi Takayanagi
    • Journal Title

      Sci ImmunoI

      Volume: 2 Pages: 5-165

    • DOI
      10.1126/sciimmunol.aan5165
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure of the Rpn13-Rpn2 complex provides insights for Rpn13 and Uch37 as anticancer targets.2017

    • Author(s)
      Lu X, Nowicka U, Sridharan V, Liu F, Randles L, Hymel D, Dyba M, Tarasov SG, Tarasova NI, Zhao XZ, Hamazaki J, Murata S, Burke TR Jr. Walters KJ.
    • Journal Title

      Nat. Commun.

      Volume: 8 Pages: 15540-15540

    • DOI
      10.1038/ncomms15540
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mutations in the deubiquitinase gene USP8 cause Cushing's disease2017

    • Author(s)
      Reincke, M., Sbiera, S., Hayakawa, A., Theodoropoulou, M., Osswald, A., Beuschlein, F., Meitinger, T., Mizuno-Yamasaki, E., Kawaguchi, K., Saeki, Y., Tanaka, K., Wieland, T., Graf, E., Saeger, W., Ronchi, C. L., Allolio, B., Buchfelder, M., Strom, T. M., Fassnacht, M., and Komada M.
    • Journal Title

      Mol Cell Biol.

      Volume: 47 Pages: 195-195

    • DOI
      10.1038/ng.3166
    • Related Report
      2017 Annual Research Report 2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Endosomal phosphatidylserine is critical for the YAP signalling pathway in proliferating cells.2017

    • Author(s)
      Matsudaira T, Mukai K, Noguchi T, Hasegawa J, Hatta T, Iemura SI, Natsume T, Miyamura N, Nishina H, Nakayama J, Semba K, Tomita T, Murata S, Arai H, Taguchi T.
    • Journal Title

      Nat. Commun.

      Volume: 8(1) Pages: 1246-1246

    • DOI
      10.1038/s41467-017-01255-3
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] In Vivo Ubiquitin Linkage-type Analysis Reveals that the Cdc48-Rad23/Dsk2 Axis Contributes to K48-linked Chain Specificity of the Proteasome in Yeast.2017

    • Author(s)
      Tsuchiya, H., Ohtake, F., Arai, N., Kaiho, A., Yasuda, S., Tanaka, K., and Saeki, Y.
    • Journal Title

      Mol Cell

    • Description
      【掲載確定】 in press
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HTLV-1 Tax induces formation of the active macromolecular IKK complex by generating Lys63- and Metl-linked hybrid polyubiquitin chains.2017

    • Author(s)
      Shibata, Y., Tokunaga, F., Goto, E., Komatsu, G., Gohda, J., Saeki, Y., Tanaka, K., Takahashi, H., Sawasaki, T., Inoue, S., Oshiumi, H., Seya, T., Nakano, H., Tanaka, Y., Iwai, K., and Inoue, JI.
    • Journal Title

      PLoS Pathog.

      Volume: 13 Issue: 12

    • DOI
      10.1371/journal.ppat.1006162
    • NAID
      120005980879
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] VCP/Cdc48 rescues the growth defect of a GPI10 mutant in yeast.2016

    • Author(s)
      Ohnuma, Y., Takata, T., Kawawaki, J., Yasuda, K., Tanaka, K., Kimura Y., and Kakizuka, A.
    • Journal Title

      FEBS Lett.

      Volume: 589 Pages: 576-580

    • DOI
      10.1016/j.febslet.2015.01.017
    • NAID
      120005531440
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The Ankrd13 family of ubiquitin-interacting motif-bearing proteins regulates valosin-containing protein/p97 protein-mediated lysosomal trafficking of caveolin I.2016

    • Author(s)
      Burana, D., Yoshihara, H., Tanno, H., Yamamoto, A., Saeki, Y., Tanaka, K., and Komada, M.
    • Journal Title

      J Biol Chem.

      Volume: 291 Pages: 6218-6231

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Specialized proteasome subunits have an essential rele in the thymie selection of CD8 (+) T cells.2016

    • Author(s)
      Kincaid, E. Z., Murata, S., Tahaka, K., and Rock, K. L.
    • Journal Title

      Nat Immunol.

      Volume: 17 Pages: 938-945

    • DOI
      10.1038/ni.3480
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] p62/Sqstml promotes malignancy of HCV-positive hepatocellular carcinoma through Nrf2-dependent metabolic reprogramming.2016

    • Author(s)
      Saito, T., Ichimura, Y., Taguchi, K., Suzuki, T., Mizushima, T., Takagi, K., Hirose, Y., Nagahashi, M., Iso, T., Fukutomi, T., Ohishi, M., Endo, K., Uemura, T., Nishito, Y., Okuda, S., Obata, M., Kouno, T., Imamura, R., Tada, Y., Obata, R., Yasuda, D., Takahashi, K., Fujimura, T., Pi, J., Lee, M-S., Ueno, T., Ohe, T., Mashino, T., Wakai, T., Kojima, H., Okabe, T., Nagano, T., Motoheshi, H., Waguri, S., Soga, T., Yamamoto, M., Tanaka, K., and Komatsu, M.
    • Journal Title

      Nat Commun.

      Volume: 7 Pages: 12-030

    • DOI
      10.1038/ncomms12030
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Unexpected mitochondrial matrix localization of Parkinson's disease-related DJ-Imutants but not wild type DJ-1.2016

    • Author(s)
      Kojima, W., Kujuro, Y., Okatsu, K., Bruno, Q., Koyano, F., Kimura, M., Yamano, K., Tanaka, K., and Matsuda, N.
    • Journal Title

      Genes Cells

      Volume: 21 Pages: 772-788

    • DOI
      10.1111/gtc.12382
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Biallelic variants in UBA5 link dysfunctional UFM1 ubiquitin-like modifier pathway to encephalopathy with movement disorder and epilepsy2016

    • Author(s)
      Muona, M., Ishimura, R., Laari, A., Ichimura, Y., Linnankivi, T., Keski-Filppula, R., Herva, R., Rantala, H., Paetau, P., Poyhonen, M., Obata, M., Uemura, T., Karnu, T., Bizen, N., Takebayashi, H., Anttonen, A-K., Tanaka, K., Palotie, A., Waguri, S., Lehesjoki, A-E., and Komatsu, M.
    • Journal Title

      AJHG (The American Journal of Human Gcnetics)

      Volume: 99 Pages: 683-694

    • DOI
      10.1016/j.ajhg.2016.06.020
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Structural analysis of a function-associated loop mutant of the substrate-recognition domain-of Fbsi ubiquitin ligase.2016

    • Author(s)
      Nishio, K., Yoshida, Y., Tanaka, K., and Mizushima, T.
    • Journal Title

      Acta Crystallogr. F Struct. Biol. Commun.

      Volume: 72 Pages: 619-626

    • DOI
      10.1107/s2053230x16011018
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The K48-K63 branched ubiquitin chain regulates NF-kB signaling2016

    • Author(s)
      Ohtake, F., Saeki, Y., Ishido, S., Kanno, J., and Tanaka, K.
    • Journal Title

      Molecular Cell

      Volume: 64 Pages: 251-266

    • DOI
      10.1016/j.molcel.2016.09.014
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The ubiquitin signal and autophagy : an orchestrated dance leading to mitochondrial degradation2016

    • Author(s)
      Koji Yamano, K., Matsuda, N., and Tanaka, K.
    • Journal Title

      EMBO Reports

      Volume: 17 Pages: 300-3016

    • DOI
      10.15252/embr.201541486
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The aspartyl protease DDI2 activates Nrf1 to compensate for proteasome dysfunction.2016

    • Author(s)
      Koizumi, S., Irie, T., Hirayama, S., Sakurai, Y., Yashiroda, H., Naguro, I., Iohijo, H., Hamazaki, J., and Murata, S.
    • Journal Title

      eLife

      Volume: 18357 Pages: 18357-18357

    • DOI
      10.7554/elife.18357
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Thymus epithelium conditions antigen responsiveness in CD8+ T cells2016

    • Author(s)
      Takada, K., Laethem, F-V, m Xing, Y., Akane, K., Suzuki, H., Murata, S., Tanaka, K., Jameson, S. C., Singer, A., and Takahama, Y.
    • Journal Title

      Nat Immunol.

      Volume: 16 Pages: 1069-1076

    • DOI
      10.1038/ni.3237
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The ankrd13 family of ubiquitin-interacting motif-bearing proteins regulates valosin-containing protein/p97 protein-mediated lysosomal trafficking of caveolin 1.2016

    • Author(s)
      Burana, D., Yoshihara, H., Tanno, H., Yamamoto, A., Saeki, Y., Tanaka, K., and Komada, M.
    • Journal Title

      J Biol Chem.

      Volume: 291 Pages: 6218-6231

    • DOI
      10.1074/jbc.m115.710707
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Essential role of specialized proteasome subunits in thymic selection of CD8 T cells2016

    • Author(s)
      Kincaid, E. Z. Murata, S., Tanaka, K., and Rock, K. L.
    • Journal Title

      Nat Immunol

    • Description
      【掲載確定】 in press
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] p62/Sqstml promotes malignancy of HCV-positive hepatocellular carcinoma through Nrf2-dependent metabolic reprogramming.2016

    • Author(s)
      Saito, T., Ichimura, Y., Taguchi, K., Suzuki, T., Mizushima, T., Takagi, K., Hirose, Y., Nagahashi, M., Iso, T., Fukutomi, T., Ohishi, M., Endo, K., Uemura, T., Nishito, Y., Okuda, S., Obata, M., Kouno, T., Imamura, R., Tada, Y., Obata, R., Yasuda, D., Takahashi, K., Fujimura, T., Pi, J., Lee, M-S., Ueno, T., Ohe, T., Mashino, T., Wakai, T., Kojima, H., Okabe, T., Nagano, T., Motohashi, H., Waguri, S., Soga, T., Yamamoto, M., Tanaka, K., and Komatsu, M.
    • Journal Title

      Nat Commun.

    • Description
      【掲載確定】 in press
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The significant role of autophagy in the granular layer in normal skin differentiation and hair growth.2015

    • Author(s)
      Yoshihara, N., Ueno, T., Takagi, A., Trejo, J. A., Haruna, K., Suga, Y., Komatsu, M., Tanaka, K., and Ikeda S.
    • Journal Title

      Arch Dermatol Res.

      Volume: 307 Pages: 159-169

    • DOI
      10.1007/s00403-014-1508-0
    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ubiquitn acetylation inhibits polyubiquitin chain elongation2015

    • Author(s)
      Ohtake, F., Saeki, Y., Sakamoto, K., Ohtake, K., Nishikawa, H., Tsuchiya, H., Ohta, T., Tanaka, K., and Kanno, J.
    • Journal Title

      EMBO Rep.

      Volume: 16 Pages: 192-201

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      10.15252/embr.201439152
    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The unexpected role of polyubiquitin chains in the formation of fibrillar aggregates2015

    • Author(s)
      Morimoto, D., Walinda, E. Fukada, H., Sou, Y. S., Kageyama, S., Hoshino, M., Fujii, T., Tsuchiya, H., Saeki, Y., Arita, K., Ariyoshi, M., Tochio, H., Iwai, K., Namba, K., Komatsu, M., Tanaka, K., and Shirakawa M.
    • Journal Title

      Nat Commun.

      Volume: 6 Pages: 000-000

    • DOI
      10.1038/ncomms7116
    • NAID
      120005528125
    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Unconventional PINKI localization mechanism to the outer membrane of depolarized mitochondria drives Parkin recruitment2015

    • Author(s)
      Okatsu, K., Kimura, M., Oka, T., Tanaka, K., and Matsuda, N.
    • Journal Title

      J Cell Sci.

      Volume: 128 Pages: 964-978

    • DOI
      10.1242/jcs.161000
    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A comprehensive method for detecting ubiquitinated substrates using TR-TUBE2015

    • Author(s)
      Yoshida, Y., Yasushi Saeki, Y., Murakami, A., Kawawaki, J., Tsuchiya, H., Yoshibara, H., Shindo, M., and Tanaka, K.
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 112 Pages: 4630-4635

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      10.1073/pnas.1422313112
    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Nedd4-induced mono-ubiquitination of IRS-2 enhances IGF-dependent signals and mitogenic activity2015

    • Author(s)
      Fukushima, T., Yoshihara, H., Furuta, H., Kamei, H., Hakuno, F., Luan, J., Duan, C., Saeki, Y., Tanaka, K., Iemura, S., Natsume, T., Chida, K., Nakatsu, Y., Kamata, H., Asano, T., and Takabashi, S-I
    • Journal Title

      Nature Commun.

      Volume: 6 Pages: 6780-6780

    • DOI
      10.1038/ncomms7780
    • NAID
      120006348510
    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Thymoproteasomes produce unique peptide motifs for positive selection of CD8+ T cells2015

    • Author(s)
      Sasaki, K., Takada, K., Ohte, Y., Kondo, H., Sorimachi, H., Tanaka, K., Takahama, Y., and Murata, M.
    • Journal Title

      Nat Commun.

      Volume: 6 Pages: 7478-7478

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      10.1038/ncomms8484
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The E3 ubiquitin ligase TRIM23 regulates adipocyte differentiation via stabilization of the adipogenic activator PPARγ2015

    • Author(s)
      Watanabe, M., Takahashi, H., Saeki, Y., Ozaki, T., Itoh, S., Suzuki, M., Mizushima, W., Tanaka, K., and Hatakeyama, S.
    • Journal Title

      eLIfe

      Volume: 4

    • DOI
      10.7554/elife.05615
    • NAID
      120005617283
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Measurement of proteasome activity in peripheral blood mononuclear cells as an indicator of susceptibility to bortezomib-induced severe neurological adverse events in patients with multiple myeloma2015

    • Author(s)
      Cho, Y., Hori, M., Okoshi, Y., Fujisawa, F., Shinagawa, A., Kudo, D., Komeno, T., Yoshida, C., Katsura, Y., Ota, I., Shimizu, S., Kamoshita, M., Sasaki, K., Tanaka, K., Mukai, H. Y., and Kojima, H.
    • Journal Title

      Acta Haematol.

      Volume: 134 Pages: 25-31

    • DOI
      10.1159/000369445
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Conserved mode of yeast Brol family V domains for interaction with YP(X) nL motif-containing target proteins2015

    • Author(s)
      Kimura, Y., Tanigawac, M., Kawawaki, J., Takagi, K., Mizushima, T., Maeda, T., and Tanaka, K.
    • Journal Title

      Eukaryotic Cell

      Volume: 14 Pages: 976-982

    • DOI
      10.1128/ec.00091-15
    • NAID
      120005671359
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Site-specific interaction mapping of phosphorylated ubiquitin to uncover parkin activation2015

    • Author(s)
      Yamano, K., Queliconi, B. B., Koyano, F., Saeki, Y., Hirokawa, T., Tanaka, K., and Matsuda, N.
    • Journal Title

      J Biol Chem.

      Volume: 290 Pages: 25199-211

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      10.1074/jbc.m115.671446
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
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    • Author(s)
      Kumanomidou, T., Nishio, K., Takagi, K., Nakagawa, T., Suzuki, A., Yamane, T., Tokunaga, F., Iwai, K., Murakami, A., Yoshida, Y., Tanaka, K., and Mizushima, T.
    • Journal Title

      PRoS ONE

      Volume: 10

    • DOI
      10.1371/journal.pone.0140366
    • NAID
      120005763414
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Liganded ERαstimulates the E3 ubiquitin ligase activity of UBE3C to facilitate cell proliferation2015

    • Author(s)
      Okada, M., Ohtake, F., Nishikawa, H., Wu, W., Saeki, Y., Tanaka, K., and Ohta, T.
    • Journal Title

      Mol Endocrinol.

      Volume: 29 Pages: 1646-1657

    • DOI
      10.1210/me.2015-1125
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Parkin receptor on damaged mitochondria revisited -uncovering the role of phosphorylated ubiquitin chain2015

    • Author(s)
      Matsuda, N. and Tanaka, K.
    • Journal Title

      Autophagy

      Volume: 11 Pages: 1700-1701

    • DOI
      10.1080/15548627.2015.1071760
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tagged tags engage disposal2015

    • Author(s)
      Matsuda, N. and Tanaka, K.
    • Journal Title

      Nature (News and Views)

      Volume: 524 Pages: 294-295

    • DOI
      10.1038/nature15199
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteasome Impairment Induces Recovery of Mitochondrial Membrane Potential and an Alternative Pathway of Mitochondrial Fusion2015

    • Author(s)
      Shirozu R, Yashiroda H, Murata S.
    • Journal Title

      Mol Cell Biol

      Volume: 36 Pages: 347-362

    • DOI
      10.1128/mcb.00920-15
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Redundant Roles of Rpn10 and Rpn13 in Recognition of Ubiquitinated Proteins and Cellular Homeostasis2015

    • Author(s)
      Hamazaki J. Hirayama S. Murata S
    • Journal Title

      Plos Genet

      Volume: 11

    • DOI
      10.1371/journal.pgen.1005401
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The thymic cortical epithelium determines the TCR repertoire of IL-17-producing γδT cells2015

    • Author(s)
      Nitta T, Muro R, Shimizu Y, Nitta S, Oda H, Ohte Y, Goto M, Yanobu- Takanashi R, Narita T, Takayanagi H, Yasuda H, Okamura T, Murata S, Suzuki H
    • Journal Title

      EMBO Rep

      Volume: なし Pages: 638-653

    • DOI
      10.15252/embr.201540096
    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Identification of minimum Rpn4-responsive elements in genes related to proteasome functions2015

    • Author(s)
      Shirozu R, Yashiroda H, Murata S
    • Journal Title

      FEBS Lett.

      Volume: 589 Pages: 933-940

    • DOI
      10.1016/j.febslet.2015.02.025
    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] N-terminal α7 deletion of the proteasome 20S core particle substitutes for yeast PI31 function2015

    • Author(s)
      Yashiroda H, Toda Y, Otsu S, Takagi K, Mizushima T, Murata S
    • Journal Title

      Mol Cell Biol

      Volume: 35(1) Pages: 141-152

    • DOI
      10.1128/mcb.00582-14
    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Sirtl-deficiency causes defective protein quality control2015

    • Author(s)
      Tomita T, Hamazaki J, Hirayama S, McBurney MW, Yashiroda H, Murata S
    • Journal Title

      Sci Rep

      Volume: 5 Pages: 12613-12613

    • DOI
      10.1038/srep12613
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Muscle Segment Homeobox Genes Direct Embryonic Diapause by Limiting Inflammation in the Uterus2015

    • Author(s)
      Cha J, Burnum-Johnson KE, Bartos A, Li Y, Baker Es, Tilton SC, Webb-Robertson Bj, Piehowski PD, Monroe ME, Jegga AG, Murata S, Hirota Y, Dey SK
    • Journal Title

      J Biol Chem

      Volume: 290 Pages: 15337-15349

    • DOI
      10.1074/jbc.m115.655001
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] PARK2/Parkin-mediated mitochondrial clearance contributes to proteasome activation during slow-twitch muscle atrophy via NFE2L1 nuclear translocation2015

    • Author(s)
      Furuya N, Ikeda SI, Sato S, Soma S, Ezaki J, Trejo JA, Takeda-Ezaki M, Fujimura T, Arikawa-Hirasawa E, Tada N, Komatsu M, Tanaka K, Kominami E, Ueno T.
    • Journal Title

      Physiological Reports

      Volume: 10 Pages: 631-41

    • DOI
      10.4161/auto.27785
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Characterization of the testis-specific proteasome subunit α4s in mammals.2015

    • Author(s)
      Hiroyuki Uechi, Jun Hamazaki, and Shigeo Murata
    • Journal Title

      J. Biol. Chem.

      Volume: 289 Pages: 12365-1234

    • DOI
      10.1074/jbc.m114.558866
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Thymoproteasomes produce unique peptide motifs for positive selection of CD8+ T cells2015

    • Author(s)
      Katsuhiro Sasakil, Kensuke Takada, Yuki Ohte, Hiroyuki Kondo, Hiroyuki Sorimachi, Keiji Tanaka, Yousuke Takahama, Shigeo Murata
    • Journal Title

      Nat Commun. in press

    • Description
      印刷中
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis for proteasome formation controlled by an assembly chaperone Nas22014

    • Author(s)
      Satoh T, Saeki Y, Hiromoto T, Wang YH, Uekusa Y, Yagi H, Yoshihara H, Yagi-Utsumi M, Mizushima T, Tanaka K, Kato K
    • Journal Title

      Structure

      Volume: (掲載確定) Pages: 731-743

    • DOI
      10.1016/j.str.2014.02.014
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] LC3B is indispensable for selective autophagy of p62 but not basal autophagy2014

    • Author(s)
      Maruyama Y, Sou YS, Kageyama S, Takahashi T, Ueno T, Tanaka K, Komatsu M, Ichimura Y.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 446 Pages: 309-315

    • DOI
      10.1016/j.bbrc.2014.02.093
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative live-cell imaging reveals molecular dynamics and cytoplasmic assembly of the 26S proteasome2014

    • Author(s)
      Pack, CG., Yukii, H., Toh-e, A., Kudo, T., Tsuchiya, H., Kaiho, A., Sakata, E., Murata, S., Sako, Y., Baumeister, W., Tanaka, K., and Saeki, Y.
    • Journal Title

      Nat Commun

      Volume: 5 Pages: 3396-3396

    • DOI
      10.1038/ncomms4396
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dissection of the role of p62/Sqstml in activation of Nrf2 during xenophagy2014

    • Author(s)
      Ishimura, R., Tanaka, K., and Komatsu, M.
    • Journal Title

      FEBS Lett

      Volume: 588 Pages: 822-828

    • DOI
      10.1016/j.febslet.2014.01.045
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ubiauitin is phosphorylated by PINK1 to activate parkin2014

    • Author(s)
      Koyano, F., Okatsu, K., Kosako, H., Tamura, Y., Go, E., Kimura, M., Kimura, Y., Tsuchiya, H., Yoshihara, H., Hirokawa, T., Endo, T., Fon. E. A., Trempe, J. F., Saeki, Y., Tanaka, K., and Matsuda, N.
    • Journal Title

      Nature

      Volume: 510(in press) Pages: 162-166

    • DOI
      10.1038/nature13392
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Pba3-Pba4 heterodimer acts as a molecular matchmaker in proteasome α-ring formation.2014

    • Author(s)
      Takagi, K., Saeki, Y., Yashiroda, H., Yagi, H., Kaiho, A., Murata, S., Yamane, T., Tanaka, K., Mizushima, T. and Kato, K.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 450 Pages: 1110-1114

    • DOI
      10.1016/j.bbrc.2014.06.119
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] ESCRT-III adaptor protein Bro1 controls functions of regulator for free ubiquitin chains 1 (Rfu1) in ubiquitin homeostasis2014

    • Author(s)
      Kimura Y, Kawawaki J, Kakiyama Y, Shimoda A, and Tanaka K
    • Journal Title

      Journal of Biological Chemistry

      Volume: 289 Pages: 21760-21769

    • DOI
      10.1074/jbc.m114.550871
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Backbone 1H, 13C, and 15N assignments of yeast Umpl, an intrinsically disordered protein that functions as a proteasome assembly chaperone2014

    • Author(s)
      Y.Uekusa, K.Okawa, M.Yagi-Utsumi, O.Serve, Y. Nakagawa, T.Mizushima, H.Yagi, Y.Saeki, K.Tanaka, and K.Kato
    • Journal Title

      Biomol NMR Assign

      Volume: 8 Pages: 383-386

    • DOI
      10.1007/s12104-013-9523-1
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Proteasome Dysfunction Activates Autophagy and the Keap1–Nrf2 Pathway Shun2014

    • Author(s)
      Kageyama S, Sou YS, Uemura T, Kametaka S, Saito T, Ishimura R, Kouno T, Bedford L, Mayer RJ, Lee MS, Yamamoto M, Waguri S, Tanaka K, and Komatsu M.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 289 Pages: 24944-24955

    • DOI
      10.1074/jbc.m114.580357
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Modification of ASCI by UFMl is crucial for ER・transactivation and breast cancer development2014

    • Author(s)
      Yoo, HM., Kang, SH., Kim, JY., Lee, JE., Seong, MW., Lee, SW., Ka, SH., Sou,YS., Komatsu, M., Tanaka, K., Lee, ST., Noh, DY., Baek, SH., Jeon, YJ., *Chung, CH.
    • Journal Title

      Mol Cell

      Volume: 56 Pages: 261-274

    • DOI
      10.1016/j.molcel.2014.08.007
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Assembly mechanisms of specialized core particles of the proteasome2014

    • Author(s)
      Bai M, Zhao X, Sahara K, Ohte Y, Hirano Y, Kaneko T, Yashiroda H, Murata S.
    • Journal Title

      Biomolecules

      Volume: 4(3) Pages: 662-677

    • DOI
      10.3390/biom4030662
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The mechanism for molecular assembly of the proteasome2014

    • Author(s)
      Sahara K, Kogleck L, Yashiroda H, Murata S.
    • Journal Title

      Adv Biol Regul

      Volume: 54 Pages: 51-58

    • DOI
      10.1016/j.jbior.2013.09.010
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] プロテアソーム ~四半世紀の研究を回想する~2018

    • Author(s)
      田中啓二
    • Organizer
      徳島大学 先端酵素学研究所設立記念シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] The Proteasome~Structure and Immunological Roles~2018

    • Author(s)
      Keiji Tanaka
    • Organizer
      The XVI International Symposium on Amyloidosis (ISA2018)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Cell-mediated Immunity govern by Immunoproteasome and Thymoproteasome, “Ubiquitin Family, Autophagy and Diseases”2018

    • Author(s)
      Keiji Tanaka
    • Organizer
      Cold Spring Harbor Asia Conference (CSH Asia)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The Proteasome ~Action Mechanisms and Physiology~2018

    • Author(s)
      Keiji Tanaka
    • Organizer
      Advanced Forum on Protein Sciences in Beijing
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Spatio-temporal Dynamics of Human Proteasomes in Stress Responses2018

    • Author(s)
      Keiji Tanaka
    • Organizer
      The 8th Proteasome and Autophagy Workshop
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] How cells respond to proteasome impairment2018

    • Author(s)
      Shigeo Murata
    • Organizer
      The proteasome hub : fine tuning of proteolysis according to cellular needs
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] タンパク質分解と生命医科学研究2017

    • Author(s)
      田中啓二
    • Organizer
      平成28年度 東京都医師会功労賞表彰式および医学研究賞・グループ研究賞受賞記念講演会
    • Place of Presentation
      東京都医師会館(東京都千代田区)
    • Year and Date
      2017-02-25
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] プロテアソームと適応(細胞性)免疫2017

    • Author(s)
      田中啓二
    • Organizer
      第14回Osteoimmunology Forum
    • Place of Presentation
      ステーションコンファレンス東京(東京都千代田区)
    • Year and Date
      2017-02-11
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] プロテアソーム : 多彩な生命活動を支えるタンパク質分解装置のバイオロジー2017

    • Author(s)
      村田 茂穂
    • Organizer
      高等研プロジェクト「生命活動を生体高分子への修飾から俯瞰する」
    • Place of Presentation
      国際高等研究所(京都府木津川市)
    • Year and Date
      2017-01-23
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 私のプロテアソーム研究 ~幸運と不運の間で~2017

    • Author(s)
      田中啓二
    • Organizer
      第44回神戸ラボ全体研究会議
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Emerging Role of Thymoproteasomes in (Adaptive) Immunity.2017

    • Author(s)
      Keiji Tanaka
    • Organizer
      Sorak Conference on Protein Metabolism and Degradation
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] プロテアソームを手探りに免疫の世界を楽しむ2017

    • Author(s)
      田中啓二
    • Organizer
      第19回免疫サマースクール in 湘南
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] プロテアソーム と オートファジー2017

    • Author(s)
      田中啓二
    • Organizer
      第22回日本病態プロテアーゼ学会学術集会 教育講演
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 細胞内リサイクルシステム~ プロテアソーム と オートファジー2017

    • Author(s)
      田中啓二
    • Organizer
      ~製薬コンソ・AMED共催 創薬コンソ分野別研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] プロテアソーム~もう一つのタンパク質リサイクルシステム2017

    • Author(s)
      田中啓二
    • Organizer
      ~東工大栄誉教授・大隅良典氏 ノーベル賞受賞記念「七人の侍」講演会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] タンパク質リサイクルシステム~プロテアソーム と オートファジー2017

    • Author(s)
      田中啓二
    • Organizer
      ~ライフサイエンス イノベーション発信セミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] プロテアソーム ~基礎研究が未来を拓く~2017

    • Author(s)
      田中啓二
    • Organizer
      第1回日本医療研究開発大賞 受賞記念講演
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] The Proteasome : Overview of the Structure and Functions2017

    • Author(s)
      田中啓二
    • Organizer
      ConBio2017 Consortium of Biological Sciences 2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Activation of Nrfl compensates for proteasome dysfunction2017

    • Author(s)
      Shigeo Murata
    • Organizer
      EMBO Conference “Protein quality control : success and failure in health and disease”
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 半端なベクトルに出口無し!~徹底した基礎研究から広がる応用~「黎明期のプロテアソーム研究」2016

    • Author(s)
      田中啓二
    • Organizer
      第39回分子生物学会(MBSJ)年会 プレナリーモーニングセミナー
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-12-02
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Identification of molecules involved in Nrf1 activation in response to proteasome inhibition2016

    • Author(s)
      村田 茂穂
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      バシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] Regulation mechanism of proteasome activity (Keynote lecture)2016

    • Author(s)
      Shigeo Murata
    • Organizer
      3rd proteostasis action meeting "Proteostasis and its Biological Implications"
    • Place of Presentation
      Lisbon. Portugal
    • Year and Date
      2016-11-02
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ~タンパク質のゆりかごから墓場まで~「プロテアソーム“自己と非自己”識別の根源に迫る!」2016

    • Author(s)
      田中啓二
    • Organizer
      京都産業大学「タンパク質動態研究所」開設記念シンポジウム
    • Place of Presentation
      京都産業大学講堂(京都府京都市)
    • Year and Date
      2016-10-26
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 免疫型プロテアソームによる Antigen processingと“自己と非自己の識別”機構2016

    • Author(s)
      田中啓二
    • Organizer
      第25回日本組織適合性学会 : MHC研究の進歩 : 生命科学と臨床医学へのインパクト
    • Place of Presentation
      北海道大学学術交流会館(北海道札幌市)
    • Year and Date
      2016-10-22
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 私のプロテアソーム研究2016

    • Author(s)
      田中啓二
    • Organizer
      89回 日本生化学会大会 ~生化学の創造的復興~ Meet the Expert.
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2016-09-27
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Spatio-temporal Dynamics of the Human Proteasome in Stress Responses2016

    • Author(s)
      Keiji Tanaka
    • Organizer
      FASEB “Ubiquitin & Cellular Regulation”
    • Place of Presentation
      Big Sky Resort Huntley Lodge 50 Big Sky Resort Road Big Sky MT, USA
    • Year and Date
      2016-06-12
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Identification of molecules involved in Nrfl activation in response to proteasome inhibition2016

    • Author(s)
      Shigeo Murata, Shun Koizumi, Jun Hamazaki
    • Organizer
      FASEB SRC "Ubiquitin & Cellular Regulation"
    • Place of Presentation
      Big Sky, Montana, USA
    • Year and Date
      2016-06-12
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ubiquitin Chain-type. 「Selectivity of Ubiquitin-binding Proteins in Yeast」2016

    • Author(s)
      Keiji Tanaka
    • Organizer
      Ubiquitin Family, Autophagy and Disease. Cold Spring Harbor Asia Conference (CSH Asia)
    • Place of Presentation
      Suzhou, China
    • Year and Date
      2016-04-18
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Regulation mechanism of proteasome activity2016

    • Author(s)
      Shigeo Murata
    • Organizer
      Ubiquitin Family, Autophagy and Disease. Cold Spring Harbor Asia Conference (CSH Asia)
    • Place of Presentation
      Suzhou, China
    • Year and Date
      2016-04-18
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Ubiquitin chain-type selectivity of ubiquitin-binding proteins in yeast2016

    • Author(s)
      Keiji Tanaka
    • Organizer
      Ubiquitin Family, Autophagy and Diseases Cold Spring Harbor Asia Conference (CSH Asia)
    • Place of Presentation
      Suzhou, China
    • Year and Date
      2016-04-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Regulatory mechanism of the proteasome2016

    • Author(s)
      Shigeo Murata
    • Organizer
      Ubiquitin Family, Autophagy and Diseases Cold Spring Harbor Asia Conference (CSH Asia)
    • Place of Presentation
      Suzhou, China
    • Year and Date
      2016-04-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] “変わる徳島大学” : 「研究と教育 ~組織改革への期待~」2016

    • Author(s)
      田中啓二
    • Organizer
      学部新設・改組及び新研究所設置記念式典・記念講演会
    • Place of Presentation
      徳島グランヴィリオホール(徳島県徳島市)
    • Year and Date
      2016-04-11
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 研究と教育~組織改革への期待~2016

    • Author(s)
      田中啓二
    • Organizer
      「変わる徳島大学」学部新設・改組及び新研究所設置記念式典・記念講演会
    • Place of Presentation
      徳島グランヴィリオホテル・グランヴィリオホール(徳島県徳島市)
    • Year and Date
      2016-04-11
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Thymoproteasome : The Key Player in Positive Selection of CD8+ T cells2016

    • Author(s)
      Keiji Tanaka
    • Organizer
      The 7th Proteasome and Autophagy Workshop
    • Place of Presentation
      Clermont-Ferrand, France
    • Year and Date
      2016-04-06
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 私のブロテアソーム研究~幸運と不運に彩られて~2016

    • Author(s)
      田中啓二
    • Organizer
      第121回日本解剖学会総会・全国学術集会
    • Place of Presentation
      ビッグパレットふくしま(福島県郡山市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] ブロテアソームの多様性と生体制御2016

    • Author(s)
      村田茂穂
    • Organizer
      第32回高峰カンファレンス
    • Place of Presentation
      如水会館(東京都千代田区)
    • Year and Date
      2016-03-11
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] ブロテアソームの多様性による生体制御2016

    • Author(s)
      村田茂穂
    • Organizer
      第4回CAKIカンファレンス
    • Place of Presentation
      リーガロイヤルホテル広島(広島県広島市)
    • Year and Date
      2016-01-09
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Regulation mechanisms of the proteasome2016

    • Author(s)
      Shigeo Murata
    • Organizer
      Ubiquitin international symposium "Diverse functions of Ubiquitin : Degradation, Signaling, and Beyond"
    • Place of Presentation
      京都大学(京都府京都市)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] In-depth Analyses of Thymoproteasomes -Positive Selection of CD8+ T cells-2015

    • Author(s)
      Keiji Tanaka
    • Organizer
      Ubiquitin and Ubiquitin-like modifiers : From molecular mechanisms and human diseases (2nd EMBO Ubiquitin Conference Series)
    • Place of Presentation
      I Cavtat, Croatia
    • Year and Date
      2015-09-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] タンパク質分解~辺境領域から生命科学の中枢へ~2015

    • Author(s)
      田中啓二
    • Organizer
      国立医薬品食品衛生研究所 講演
    • Place of Presentation
      国立医薬品食品衛生研究所(東京都世田谷区)
    • Year and Date
      2015-09-04
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Ubiquitin : lst Mitophagy driven by PINKI, Parkin and Ubiquitin, 2nd MS and TR-Tube Strategy for Ubiquitin Code Analysis2015

    • Author(s)
      Keiji Tanaka
    • Organizer
      The 2nd Ubiquitin Symposium at Hotel Stefani e
    • Place of Presentation
      Vienna Biocenter, Austria
    • Year and Date
      2015-07-10
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Basic Mechanisms and Physiopathology of The Proteasome2015

    • Author(s)
      Keiji Tanaka
    • Organizer
      IMP (Research Institute of Molecular Pathology) Lecture
    • Place of Presentation
      Vienna Biocenter, Austria
    • Year and Date
      2015-07-09
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ブロテアソームの多様性と生体制御2015

    • Author(s)
      村田茂穂
    • Organizer
      第7回免疫適塾
    • Place of Presentation
      スターツ総合研修センター(茨城県つくばみらい市)
    • Year and Date
      2015-07-05
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 栄養学における基礎科学と応用科学の統合2015

    • Author(s)
      田中啓二
    • Organizer
      徳島大学栄養学科同窓会(栄友会)東日本支部(文化功労者顕彰記念)同窓会セミナー
    • Place of Presentation
      昭和女子大学(東京都世田谷区)
    • Year and Date
      2015-06-27
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 「細胞内の蛋白質の一生 : 新生から死に至るまで」 : タンバク質分解装置“ブロテアソーム”の動態と作動機構2015

    • Author(s)
      田中啓二
    • Organizer
      第15回 日本蛋白質科学会 年会シンポジウム
    • Place of Presentation
      あわぎんホール(徳島県徳島市)
    • Year and Date
      2015-06-24
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 胸腺ブロテアソームによるT細胞正の選択の新しい機構2015

    • Author(s)
      村田茂穂
    • Organizer
      平成27年度日本生化学会関東支部例会
    • Place of Presentation
      新潟日報メディアシップ(新潟県新潟市)
    • Year and Date
      2015-06-20
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Ubiquitin Phosphorylation : a missing link between activation and recruitment of Parkin for mitophagy2015

    • Author(s)
      Keiji Tanaka
    • Organizer
      The 7th International Symposium on Autophagy
    • Place of Presentation
      Huangshan, China
    • Year and Date
      2015-03-19 – 2015-03-23
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 栄養学における基礎科学と応用科学の統合2015

    • Author(s)
      田中啓二
    • Organizer
      日本栄養士会・研究教育事業部・全国研修会の基調講演
    • Place of Presentation
      東京農業大学、東京都世田谷区
    • Year and Date
      2015-03-07
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] タンパク質分解~辺境領域から生命科学の中枢へ~2015

    • Author(s)
      田中啓二
    • Organizer
      第8回「酵素学研究拠点」シンポジウム : タンパク質代謝・分解系の酵素学「文化功労者」顕彰記念講演
    • Place of Presentation
      徳島大学藤井節郎記念ホール、徳島県徳島市
    • Year and Date
      2015-02-10
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 私のフロテアソーム研究~成功と失敗のエピソード~2014

    • Author(s)
      田中啓二
    • Organizer
      国立国際医療研究センター研究所セミナー
    • Place of Presentation
      国立国際医療研究センター、東京都新宿区
    • Year and Date
      2014-12-08
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 私のプロテアソーム研究~成功と失敗のエピソード~2014

    • Author(s)
      田中啓二
    • Organizer
      新学術領域「ユビキチンネオバイオロジー」領域班会議 -文化功労者 -顕彰記念講演
    • Place of Presentation
      ホテルたつき、愛知県蒲郡市
    • Year and Date
      2014-12-04
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Proteasome Dynamics and Physiopathology focusing on Immunity2014

    • Author(s)
      Keiji Tanaka
    • Organizer
      ZOMES VIII Conference-From systems dynamics to biology
    • Place of Presentation
      Xiamen Grand Hotel, Xiamen, China
    • Year and Date
      2014-11-18 – 2014-11-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] オートファジーの破綻によるミトコンドリアストレスと肝癌・神経変性疾患2014

    • Author(s)
      田中啓二
    • Organizer
      第9回臨床ストレス応答学会大会特別講演
    • Place of Presentation
      岡山大学鹿田キャンパス内マスカットキューブ、岡山県岡山市
    • Year and Date
      2014-11-02 – 2014-11-03
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ミスフォールドタンパク質の細胞内動態制御機構2014

    • Author(s)
      村田茂穂
    • Organizer
      第9回臨床ストレス応答学会
    • Place of Presentation
      岡山大学、岡山県岡山市
    • Year and Date
      2014-11-02
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Rpn13 and Rpn10 coordinately play a role in degradation of ubiquitinated protein in mice2014

    • Author(s)
      Shigeo Murata
    • Organizer
      EMBO-CONICET ubiquitin conference 2014 "Ubiquitin & ubiquitin-like proteins : At the crossroads from chromatin to protein"
    • Place of Presentation
      Buenos Aires, Argentina
    • Year and Date
      2014-10-22
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Surveillance of Mitochondrial Homeostasis by the PINK1-Parkin System2014

    • Author(s)
      Keiji Tanaka
    • Organizer
      EMBO Conference Ubiquitin & Ubiquitin-like Proteins : At the crossroads from chromatin to protein
    • Place of Presentation
      Buenos Aires, Argentina
    • Year and Date
      2014-10-19 – 2014-10-24
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Surveillance of Mitochondrial Homeostasis by the PINK1-Parkin System2014

    • Author(s)
      Keiji Tanaka
    • Organizer
      The 87th Annual Meeting of the Japanese Biochemical Society New Phase in Organelle Biology : Cellular Organellostasis with Mitochondria as a Hub
    • Place of Presentation
      Kyoto International Conference Center, Kyoto
    • Year and Date
      2014-10-15
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Basic Mechanisms and Physiopathological Roles of Eukaryotic Proteasomes2014

    • Author(s)
      Keiji Tanaka
    • Organizer
      Nobel Forum Lecture
    • Place of Presentation
      Karolinska Institute, Stockholm, SWEDEN
    • Year and Date
      2014-09-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 神経変性疾患の謎を解く-タンパク分解系からのアフローチー-2014

    • Author(s)
      田中啓二
    • Organizer
      第37回日本神経科学大会(Neuroscience 2014)「神経科学が世界を変える」教育講演
    • Place of Presentation
      パシフィコ横浜, 神奈川県横浜市
    • Year and Date
      2014-09-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 生化学の立場から~タンパク質の新陳代謝~2014

    • Author(s)
      田中啓二
    • Organizer
      第61回日本栄養改善学会学術集会「栄養学-新たな挑戦日本栄養改善学会60周年シンポジウム」
    • Place of Presentation
      パシフィコ横浜、神奈川県横浜市
    • Year and Date
      2014-08-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] プロテアソームの制御による生体調節2014

    • Author(s)
      村田茂穂
    • Organizer
      第19回日本病態プロテアーゼ学会学術集会
    • Place of Presentation
      千里ライフサイエンスセンター、大阪府豊中市
    • Year and Date
      2014-08-09
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 私のプロテアソーム研究~偶然が必然を誘う幸運~2014

    • Author(s)
      田中啓二
    • Organizer
      免疫サマースクール
    • Place of Presentation
      オリビアン小豆島、香川県小豆島
    • Year and Date
      2014-07-29
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] The PINK1-Parkin Pathway Controls Mitochondrial Homeostasis Whose Collapse Causes Parkinson's Disease2014

    • Author(s)
      Keiji Tanaka
    • Organizer
      International Conference on Protein Metabolism and Diseases
    • Place of Presentation
      SolBeach Hotel & Resort, Yangyang, Korea
    • Year and Date
      2014-07-09 – 2014-07-11
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 「プロテアソーム」動作原理から炎症・免疫疾患制御への展望2014

    • Author(s)
      田中啓二
    • Organizer
      第35回日本炎症・再生医学会Think Different教育講演
    • Place of Presentation
      万国津梁館、沖縄県名護市
    • Year and Date
      2014-07-01 – 2014-07-04
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] タンパク質の新陳代謝~健康と病気のみなもと~2014

    • Author(s)
      田中啓二
    • Organizer
      「サイエンステクノフロンティアフォーラム」サイテックサロン
    • Place of Presentation
      駒場ファカルティハウス(東京大学駒場キャンパス内)、東京都目黒区
    • Year and Date
      2014-06-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] The Proteasome : Basic Mechanisms and The Immunological Roles2014

    • Author(s)
      Keiji Tanaka
    • Organizer
      CBSM2014-Yonsei BK21plus Joint symposium
    • Place of Presentation
      Yonsei Univ. (Seoul) & Gunja Village(Andong), Korea
    • Year and Date
      2014-06-19 – 2014-06-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Thymoproteasome generates unique peptides that elicit positive selection of developing T cells2014

    • Author(s)
      Shigeo Murata
    • Organizer
      Cold Spring Harbor Asia Symposium "Protein Modification & Homeostasis"
    • Place of Presentation
      Suzhou, China
    • Year and Date
      2014-06-17
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 私のプロテアソーム研究を回想して2014

    • Author(s)
      田中啓二
    • Organizer
      第66回日本細胞生物学会 : 七人の侍たちからのメッセージ~私の仕事を通じて若者達に伝えたきこと~Mol Cell (preview)
    • Place of Presentation
      奈良県新公会堂・東大寺総合文化センター、奈良県奈良市
    • Year and Date
      2014-06-11 – 2014-06-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] The PINK1-Parkin System Controls Mitochondrial Homeostasis to Suppress The Cause of Parkinson's Disease2014

    • Author(s)
      Keiji Tanaka
    • Organizer
      The KSU International Symposium on Cutting-Edge of Life Sciences
    • Place of Presentation
      Musubiwaza-hall at Mibu Campus of KSU, Kyoto
    • Year and Date
      2014-05-30 – 2014-05-31
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Book] Ubiquitin chain elongation : An intriguing strategy. Mol Cell (preview)2014

    • Author(s)
      Iwai, K. and Tanaka, K.
    • Total Pages
      3
    • Publisher
      Cell Press
    • Related Report
      2014 Annual Research Report
  • [Book] FROM EAST TO WEST : Pioneers in Parkinson's disease in Japan-A historical overview of major achievement in research2014

    • Author(s)
      Tanaka, K.
    • Total Pages
      2
    • Publisher
      QOL laboratory Corp
    • Related Report
      2014 Annual Research Report
  • [Remarks] 公益財団法人東京都医学総合研究所 蛋白質代謝研究室

    • URL
      http://www.igakuken.or.jp/pro-meta/
    • Related Report
      2017 Annual Research Report
  • [Remarks] 東京大学大学院薬学系研究科 蛋白質代謝学教室

    • URL
      http://www.f.u-tokyo.ac.jp/~tanpaku/
    • Related Report
      2017 Annual Research Report
  • [Remarks]

    • URL
      http://www.f.u-tokyo.ac.jp/~tanpaku/
    • Related Report
      2016 Annual Research Report
  • [Remarks]

    • URL
      http://www.igakuken.or.jp/pro-meta/
    • Related Report
      2016 Annual Research Report
  • [Remarks]

    • URL
      http://www.f.u-tokyo.ac.jp/~tanpaku/
    • Related Report
      2015 Annual Research Report
  • [Remarks]

    • URL
      http://www.igakuken.or.jp/pro-meta/
    • Related Report
      2015 Annual Research Report
  • [Remarks] 研究代表者 : 田中啓二(蛋白質代謝研究室)

    • URL
      http://www.igakuken.or.jp/pro-meta/
    • Related Report
      2014 Annual Research Report
  • [Remarks] ユビキチンプロジェクト

    • URL
      http://www.igakuken.or.jp/project/detail/ubiquitin.html
    • Related Report
      2014 Annual Research Report
  • [Remarks] 研究分担者 : 村田茂穂

    • URL
      http://www.f.u-tokyo.ac.jp/~tanpaku/
    • Related Report
      2014 Annual Research Report
  • [Patent(Industrial Property Rights)] ポリユビキチン化基質の同定方法2014

    • Inventor(s)
      吉田雪子、佐伯泰、土屋光、村上有沙、田中啓二
    • Industrial Property Rights Holder
      (公財)東京都医学総合研究所
    • Filing Date
      2014-11-13
    • Application Year
      2014
    • Related Report
      2014 Annual Research Report

URL: 

Published: 2014-04-24   Modified: 2019-07-29  

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