| Budget Amount *help |
¥182,000,000 (Direct Cost: ¥140,000,000、Indirect Cost: ¥42,000,000)
Fiscal Year 2018: ¥36,400,000 (Direct Cost: ¥28,000,000、Indirect Cost: ¥8,400,000)
Fiscal Year 2017: ¥36,400,000 (Direct Cost: ¥28,000,000、Indirect Cost: ¥8,400,000)
Fiscal Year 2016: ¥36,400,000 (Direct Cost: ¥28,000,000、Indirect Cost: ¥8,400,000)
Fiscal Year 2015: ¥36,400,000 (Direct Cost: ¥28,000,000、Indirect Cost: ¥8,400,000)
Fiscal Year 2014: ¥36,400,000 (Direct Cost: ¥28,000,000、Indirect Cost: ¥8,400,000)
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| Outline of Final Research Achievements |
Protein enzymes catalyze many pivotal chemical reactions in the body. Despite the great advances of the filed of life science so far, however, how the evolution of enzymes was proceeded is still unknown. We hypothesized that the short peptides would have been main biocatalysts in the evolutional process to the “protein world”. The purpose of this research is to generate minimal peptide catalysts based on the macrocyclic scaffold. To address this, we generated the trillions of constrained macrocyclic peptides and selected out the peptides in which have the acyltransferase activity by means of a combination of the FIT system and the mRNA display technique. After the selection rounds, we discovered the acyltransferase active macrocyclic peptides and the further thorough investigation revealed that the linear short peptide has a self-acylation activity. We believe that this finding accelerates the development of a research for the “peptide world” before the “protein world”.
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